Mouse anti caga

Rabbit anti-KDM4A, anti-ERK1/2, anti-p-ERK1/2 (T202/Y204), anti-c-Jun, and anti-p-c-Jun (S63) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-KDM4B was purchased from Bethyl Laboratories (Montgomery, TX, USA). Rabbit anti-KDM4C was purchased from Novus Biological (Littleton, CO, USA). Rabbit anti-H3K9me3 was purchased from Active Motif (Carlsbad, CA, USA). Rabbit anti-IgG, and anti-p-Tyrosine were purchased from Millipore (Billerica, MA, USA). Mouse anti-CagA, and anti-IgG were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Mouse anti-β-Actin was purchased from Sigma. Lentiviral vector pLKO-control (pLKO), pLKO-shKDM4A (sh4A#1, TRCN0000234912; sh4A#2, TRCN0000234914), -shKDM4B (sh4B#1, TRCN0000018016; sh4B#2, TRCN0000379460), -shKDM4C (sh4C#1, TRCN0000235047; sh4C#2, TRCN0000235048) plasmids were purchased from The RNAi Consortium (TRC).

The primary antibodies used for Western blot analysis were as follows: rat anti-integrin β1 clone AIIB2 (Merck, MABT409), rabbit anti-integrin α5 clone 5E18 ZooMAb (Sigma-Aldrich, ZRB1122-25UL), mouse anti-CD15s (sialyl Lewis^x) (Santa Cruz, sc-32243), mouse anti-CagA (Santa Cruz, sc-28368), mouse anti-CagA phospho (Santa Cruz, sc-508), mouse anti-VacA (Santa Cruz, sc-32746), rabbit anti-p38 MAPK (Agrigo, ARG55258), rabbit anti-p38 MAPK phospho (Agrigo, ARG51850) rabbit anti-Cox-2 (Agrigo, ARG56421), goat anti-ATF-2 (Arigo, ARG63122), rabbit anti-ATF2 phospho (Agrigo, ARG42620), mouse anti-GAPDH (Agrigo, ARG10112). Furthermore, the secondary antibodies were as follows: donkey anti-goat IgG antibody (Arigo, ARG65352), goat anti-rabbit IgG (pre-adsorbed to 10 nm gold) (Abcam, ab27234), goat anti-rabbit IgG H&L (HRP) (Merck Millipore, AP132P), goat anti-mouse IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11001), goat anti-mouse IgG (HRP) (Biosciences, 554002) Goat anti-Rat IgG (HRP) (Thermo Fisher, 31470), goat anti-rabbit (Alexa Fluor 488) (Invitrogen A11034), goat anti-rat IgG H&L cross-adsorbed secondary antibody (Alexa Fluor 488) (Thermo Fisher, A-11006), and goat anti-mouse IgM CFL488 (Santa cruz, sc-395784).