Anti sall4

Manufactured by Abcam

Sourced in United States, Japan, Germany, United Kingdom

Anti-SALL4 is a lab equipment product that detects the SALL4 protein. SALL4 is a zinc finger transcription factor that plays a role in stem cell maintenance. This product can be used for research purposes.

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Lab products found in correlation

Anti gfap, by Abcam (2 mentions) Aperio cs2 slide scanner, by Leica (2 mentions) Anti glypican 3, by Abcam (2 mentions) Vectashield mounting medium, by Vector Laboratories (2 mentions) Bca method, by Beyotime (1 mentions) Anti wnt3a, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Abcam (1 mentions) Anti vimentin, by Abcam (1 mentions) Anti β actin antibody, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibody, by Beyotime (1 mentions) Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Avantor (1 mentions) Anti cyclin d1, by Abcam (1 mentions) Bicinchoninic acid bca protein assay kit, by Beyotime (1 mentions) Anti cxcr7 antibody, by Abcam (1 mentions) Anti cxcr7, by Abcam (1 mentions) Anti cxcr4, by R&D Systems (1 mentions) Anti hk2, by Cell Signaling Technology (1 mentions) Anti pkm2, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

SALL4 (8 citations) Rabbit anti-SALL4 (2 citations)

7 protocols using anti sall4

Teratoma Histopathological Analysis

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Teratomas were fixed in 10% neutral buffered formalin for 48 h. The fixed tissues were submitted to the Histo Pathology Core at UT Southwestern for processing, embedding, and Hematoxylin and Eosin (H&E) staining. The low and high magnification H&E images of the entire section were captured with a Leica Aperio CS2 slide scanner. Areas of mature and immature elements were manually marked by a pathologist and quantified using ImageJ.
For immunohistochemistry, unstained slides of sectioned tissues were deparaffinized and epitope/antigen retrieval was performed with 10 mM sodium citrate (pH 6.0). The slides were treated with 0.3% H₂O₂ to block endogenous peroxidase. The slides were then incubated in 3% BSA and stained with the appropriate primary antibodies, including anti-SALL4 (Abcam), anti-GFAP (Abcam), and anti-Glypican-3 (Abcam). After an overnight incubation in primary antibodies, slides were stained with secondary antibodies and DAPI before mounting with the Vectashield mounting medium (Vector Laboratories). The slides were viewed with a DeltaVision microscope.

Sivakumar S., Qi S., Cheng N., Sathe A.A., Kanchwala M., Kumar A., Evers B.M., Xing C, & Yu H. (2022). TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling. Cell reports, 38(7), 110395.

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Western Blot Analysis of Cell Proteins

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Total cell proteins were extracted in RIPA lysis buffer. The protein concentration was determined by using BCA method (Biyuntian, Jiangsu, China). Equal amount of proteins were loaded on a 10 % SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gel. Following electrophoresis, the proteins were blotted to a PVDF membrane. The membrane was blocked in 5 % non-fat milk and then incubated with primary antibodies overnight at 4 °C and secondary antibody reactions for 2 h at 37 °C. Sources of primary antibodies were: anti-SALL4, anti-Vimentin and anti-E-cadherin (Abcam, Cambridge, MA, USA), anti-β-catenin, anti-wnt3a, anti-β-actin antibodies (Cell Signaling Technology, USA)

He J., Zhou M., Chen X., Yue D., Yang L., Qin G., Zhang Z., Gao Q., Wang D., Zhang C., Huang L., Wang L., Zhang B., Yu J, & Zhang Y. (2016). Inhibition of SALL4 reduces tumorigenicity involving epithelial-mesenchymal transition via Wnt/β-catenin pathway in esophageal squamous cell carcinoma. Journal of Experimental & Clinical Cancer Research : CR, 35, 98.

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Investigating SALL4 and PTEN Regulation in Cells

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Fourty-eight hours after SALL4-siRNA and negative control siRNA transfection or 24 h before addition of PTEN inhibitors pten (bpv), the whole protein lysate was prepared from cells by radioimmunoprecipitation assay lysis buffer containing 50 mM Tris/HCl pH 7.5, 0.1% sodium dodecyl sulfate (SDS), 1% Triton-X 100, 0.5–1% sodium deoxycholate, 150 mM sodium chloride, and protease inhibitors (Roche). Bicinchoninic acid (BCA) protein assay kit (Beyotime Institute of Biotechnology) was used to calculate the protein concentration. Equal amounts of protein samples were separated with 6–10% SDS-PAGE and transferred onto nitrocellulose membrane, under constant voltage of 60 V. The membrane was blocked with 5% bovine serum albumin (BSA) (Amresco, USA) for 2 h at room temperature, and then incubated with 1:1000 primary antibodies overnight at 4 °C and 1:3000 HRP-conjugated secondary antibody (Beyotime Institute of Biotechnology) for 2 h followed by washing with PBST three times for 5 min each time. The signal was detected using enhanced chemiluminescence (ECL) (Thermo). The primary antibodies used in this study were anti-SALL4, anti-cyclin D1 (Abcam, Japan), anti-PTEN, anti-PI3K, anti-p-PI3K, anti-AKT, anti-p-AKT, and anti-GAPDH (CST, USA). Repeated three times this experiments.

Liu C., Wu H., Li Y., Shen L., Yu R., Yin H., Sun T., Sun C., Zhou Y, & Du Z. (2017). SALL4 suppresses PTEN expression to promote glioma cell proliferation via PI3K/AKT signaling pathway. Journal of Neuro-Oncology, 135(2), 263-272.

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Teratoma Histopathological Analysis

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Immunohistochemical Analysis of Stem Cell Markers

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Sections were dewaxed and rehydrated as described above. After rinsing with distilled water and phosphate-buffered saline (PBS), sections were incubated with 25% goat serum in TBS containing 5% (w/v) bovine serum albumin (BSA) for 30 min at RT. Subsequently, primary antibodies against CXCR7 (rabbit polyclonal anti-CXCR7, Abcam, dilution of 1∶400, Cambridge, UK), CXCR4 (rat monoclonal anti-CXCR4, R&D System, dilution of 1∶20, Wiesbaden, Germany), LIN28a (rabbit polyclonal anti-LIN28a, A177 Cell Signaling, dilution of 1∶50, Darmstadt, Germany) and SALL4 (mouse monoclonal anti-SALL4, Abcam, dilution of 1∶150, Cambridge, UK) were applied and sections were incubated in a humid chamber at 4°C for 60 min. The specificity of the anti-CXCR7 antibody was confirmed immunohistochemically using peptide competition (Fig. S2A–D). For this, the human GPCR RDC1 peptide was applied in a ratio of 1∶1 with the anti-CXCR7 antibody to testicular tissue sections (14 dpp, incubation 1 hr at RT, Abcam, Cambridge, UK). Incubation with corresponding immunoglobulin G (IgG) fractions was used as negative control. Following two PBS washing steps the appropriate Alexa fluor 488-linked or 546-linked secondary antibodies, diluted in TBS/5% BSA, were applied for 45 min at RT in the dark. Cells were counter-stained with Hoechst for visualization of nuclei (33258, Sigma-Aldrich, Steinheim, Germany).

Westernströer B., Terwort N., Ehmcke J., Wistuba J., Schlatt S, & Neuhaus N. (2014). Profiling of Cxcl12 Receptors, Cxcr4 and Cxcr7 in Murine Testis Development and a Spermatogenic Depletion Model Indicates a Role for Cxcr7 in Controlling Cxcl12 Activity. PLoS ONE, 9(12), e112598.

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Protein Expression Analysis of Stem Cell Regulators

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The cells were washed twice with PBS and lysed with RIPA buffer containing 1% protease inhibitors. Equal amounts of proteins were separated on 12% sodium dodecyl sulfate–polyacrylamide gels and transferred onto polyvinylidene fluoride membranes, followed by blocking with 5% nonfat milk for 1 h. The membranes were incubated with primary antibodies overnight at 4 °C. The following primary antibodies were used: anti-SALL4 (1:500, ab29112, Abcam), anti-HK-2 (1:1000, 2867T, Cell Signaling Technology), anti-LDHA (1:1000, 3582T, Cell Signaling Technology), anti-PKM2 (1:1000, 4053T, Cell Signaling Technology), anti-β-actin (1:1000, 4970T, Cell Signaling Technology). After incubation with the secondary antibodies (Bioworld Technology) at 37 °C for 1 h, the bands were visualized with a chemiluminescent detection system.

Shao M., Zhang J., Zhang J., Shi H., Zhang Y., Ji R., Mao F., Qian H., Xu W, & Zhang X. (2020). SALL4 promotes gastric cancer progression via hexokinase II mediated glycolysis. Cancer Cell International, 20, 188.

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Immunohistochemical Analysis of Subcutaneous Tumor Samples

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The subcutaneous tumors of nude mice were obtained and xed in 4% formaldehyde. Then they were embedded in para n and cut into 4 um thick sections. The sections were incubated with primary antibodies, such as anti-ki67 and anti-SALL4 (Abcam, UK) overnight at 4 ℃. After being washed with PBS for three times, the sections were incubated with HRP-polymer-conjugated secondary antibody at RT for 1 h. We used 3,3'-Diaminobenzidine (DAB) solution to stain the sections for 3 min and hematoxylin to counterstain nuclei. The percentage of positive cells was determined based on three random elds of the sections.

Chang S., Sun G., Zhang D., Li Q., Wan Y., Zhou X., Lu Q., Han X., Chen X., Xing T., Liu Q., Ren Y., Zhang S., & Qian H. (2020). MiR-3622a-3p acts as a tumor suppressor in colorectal cancer by reducing stemness features and EMT through targeting spalt-like transcription factor 4.

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