Cd45r b220 pe cy7

Manufactured by Thermo Fisher Scientific

The CD45R/B220-PE-Cy7 is a fluorescent-labeled antibody that binds to the CD45R/B220 antigen. It is commonly used in flow cytometry to identify and quantify B cells in a sample.

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Close spelling variants of this product

B220-PE (19 citations) CD45R/B220 (12 citations) CD45R-PE (4 citations) PE-Cy7-B220 (3 citations) Anti-CD45R(B220)-Pe-Cy7 (2 citations)

2 protocols using cd45r b220 pe cy7

Characterizing Microbiome-Host Interactions

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EcN and Salmonella typhimurium were purchased from China General Microbiological Culture Collection Center. Caco-2 and Raji cell lines were obtained from the American Type Culture Collection. Baker’s yeast was obtained from Lesaffre. Minimum Eagle’s medium (MEM), RPMI 1640, fetal bovine serum, and antibiotic/antimycotic solution were purchased from Thermo Fisher Scientific. Calcofluor-white stain was purchased from Sigma-Aldrich. The β-Glucan assay kit was purchased from Megazyme. Fluorescence-activated cell sorting antibodies including anti-mouse CD3-PerCP Cy5.5, CD4–fluorescein isothiocyanate (FITC), CD8–allophycocyanin (APC), CD11c-FITC, CD80–phycoerythrin (PE), CD86-APC, CD45R/B220-PE-Cy7, and CD138-APC were purchased from eBioscience. IgA-PE was purchased from Thermo Fisher Scientific. Plasmids pBBR1MCS2-Tac-mCherry (kanamycin resistance) and all other reagents were purchased from domestic suppliers and used as received.

Lin S., Mukherjee S., Li J., Hou W., Pan C, & Liu J. (2021). Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches. Science Advances, 7(20), eabf0677.

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Comprehensive Immune Cell Profiling

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Primary tumors were digested using Liberase (Roche) mix in DMEM media at 37°C for 30 min. Following ACK-lysis, single-cell suspension was equally divided, and stained for either lymphoid [CD45-PacOrange (Life Technologies, #MCD4530), CD3e-APC-e780 (eBioscience, #47-0032), CD45R(B220)-PE-Cy7 (eBioscience, #25-0452), CD4-APC (BioLegend, #100412), CD8-PE (BD Pharmingen, #553033), and NK-1.1-PerCP-Cy5.5 (BioLegend, #108728)] or myeloid [CD45-PacOrange (Life Technologies, #MCD4530), CD11b-PE-Cy7 (eBioscience, #25-0112), F4/80-PE (BioLegend, #123110), Ly6C-APC-e780 (BioLegend, #128025), and Ly6G-APC (BioLegend, #127613)] panel. FxCycle-violet and 20μl CountBright™ Absolute Counting Beads (Thermo Fisher Scientific, #C36950) were added to exclude dead cells and to analyze the absolute cell numbers per mg of tissue, respectively. Samples were acquired on BD Aria FusionII and were analyzed with FlowJo software.

Pari A.A., Singhal M., Hübers C., Mogler C., Schieb B., Gampp A., Gengenbacher N., Reynolds L.E., Terhardt D., Géraud C., Utikal J., Thomas M., Goerdt S., Hodivala-Dilke K., Augustin H.G, & Felcht M. (2020). Tumor cell-derived Angiopoietin-2 promotes metastasis in melanoma. Cancer research, 80(12), 2586-2598.

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