Superdex 200 hr 10 30 gel filtration column

The molar mass of the TRAF1 TRAF domain was determined by multi angle light scattering (MALS). The target protein was loaded onto a Superdex 200 HR 10/30 gel-filtration column (GE Healthcare) that had been pre-equilibrated in buffer containing 20 mM sodium citrate at pH 5.0 and 1 M NaCl. The acta chromatography system was coupled to a MALS detector (mini-DAWM treos) and a refractive index detector (Optilab DSP) (Wyatt Technology).

The molar mass of DREP1 D62R and DREP3D167R mutants were determined by multi-angle light scattering (MALS). Target proteins were injected into a Superdex 200 HR 10/30 gel filtration column (GE Healthcare) that had been equilibrated in a buffer containing 20 mM Tris at pH 8.0 and 150 mM NaCl. The chromatography system was coupled to a three-angle light scattering detector (mini-DAWN EOS) and a refractive index detector (Optilab DSP) (Wyatt Technology). Data were collected every 0.5 s at a flow rate of 0.2 mL/min and analyzed using the ASTRA program, which gave the molar mass and mass distribution (polydispersity) of the sample.