Trizma base and acid

Imidazole, Trizma base and acid, DNase, SIGMAFAST protease tablets, NaCl, Ni²⁺-resin, anti-PKP1 antibody, DAPI (4′,6-diamidino-2-phenylindole), paraformaldehyde (PFA), and ultra-pure dioxane were obtained from Sigma (Madrid, Spain). Ampicillin, kanamycin, and isopropyl-β-D-1-thiogalactopyranoside were obtained from Apollo Scientific (Stockport, UK). Dialysis tubing with a molecular weight cut-off of 3500 Da, Triton X-100, TCEP (tris(2-carboxyethyl)phosphine) and the SDS protein marker (PAGEmark Tricolor) were obtained from VWR (Barcelona, Spain). Amicon centrifugal devices with a molecular weight cut-off of 30 kDa were obtained from Millipore (Barcelona, Spain). The rest of the materials were of analytical grade. Water was deionized and purified on a Millipore system.

Imidazole, Trizma base and acid, DNase, SIGMAFAST protease tablets, NaCl and Ni²⁺-resin were from Sigma (Madrid, Spain). The β-mercaptoethanol was from BioRad (Madrid, Spain). Ampicillin and isopropyl-β-D-1-thiogalactopyranoside were obtained from Apollo Scientific (Stockport, UK). Triton X-100, TCEP, dialysis tubing with a molecular weight cut-off of 3500 Da and the SDS protein marker (PAGEmark Tricolor) were from VWR (Barcelona, Spain). Poly(ADP-ribose) (PAR) polymer was from Trevigen (Minneapolis, USA). Amicon centrifugal devices with a molecular weight cut-off of 3 kDa were from Millipore (Barcelona, Spain). The rest of the used materials were of analytical grade. Water was deionized and purified on a Millipore system. Please see Appendix Table S1 for a complete list of reagents.

Deuterium oxide and IPTG was obtained from Apollo Scientific (Stockport, UK). Sodium trimethylsilyl [2,2,3,3-²H₄] propionate (TSP), imidazole, DNase, Trizma base and acid, yeast RNA, glutaraldehyde (25% w/v solution), ANS, deuterated acetic acid, its sodium salt and His-Select HF nickel resin were from Sigma-Aldrich (Madrid, Spain). The β-mercaptoethanol (β-ME) was from BioRad (Madrid, Spain). Triton X-100 and protein marker, PAGEmark-tricolor (G Biosciences) were from VWR (Barcelona, Spain). Dialysis tubing, with a molecular weight cut-off of 3500 Da, was from Spectrapor (Spectrum Laboratories, Breda, The Netherlands). Amicon centrifugal devices with a cut-off molecular weight of 3000 Da were from Millipore (Barcelona, Spain). Standard suppliers were used for all other chemicals. Water was deionized and purified on a Millipore system.