Cd4 h 370

Manufactured by Santa Cruz Biotechnology

Sourced in Japan

CD4 (H-370) is an antibody that recognizes the CD4 antigen. CD4 is a co-receptor expressed on the surface of T helper cells and plays a crucial role in the activation and regulation of the immune response.

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H-370; (2 citations)

2 protocols using cd4 h 370

Monoclonal antibody generation against EBAG9

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Human anti-EBAG9 monoclonal antibody was generated against a glutathione S-transferase–EBAG9 fusion protein.^{9 (link)} Antibodies against CD3 (Dako, Tokyo, Japan), CD4 (H-370; Santa Cruz Biotechnology, Dallas, DX, USA), CD8a (H-160; Santa Cruz Biotechnology), CD107-PE (1D4B; BD Pharmingen, Tokyo, Japan), CD8a–FITC (53-6.7; BD Pharmingen) and anti-β-actin (AC-74; Sigma-Aldrich, Tokyo, Japan) were purchased.

Miyazaki T., Ikeda K., Horie-Inoue K., Kondo T., Takahashi S, & Inoue S. (2014). EBAG9 modulates host immune defense against tumor formation and metastasis by regulating cytotoxic activity of T lymphocytes. Oncogenesis, 3(11), e126-.

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Multicolor Immunohistochemistry of Brain Tissue

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Brian tissue sections were incubated overnight with primary antibodies at 4°C and then with corresponding fluorochrome-conjugated secondary antibodies at room temperature for 1 h. The following primary antibodies were used: anti-human NKp46 (195314; R&D Systems), CD49a (SR84; BD Bioscience), CD57 (MA1-81071; Invitrogen), CD68 (ED1; Abcam), CD4 (H-370; Santa Cruz), CD8 (UCH-T4; Santa Cruz), CD19 (HIB19; BioLegend), CD16b (CLB-gran11.5; BD Bioscience), CD66b (polyclonal; Bioss), perforin (dG9; BioLegend), CD69 (D-3; Santa Cruz), Caspase-3 (9661; CST), CD31 (JC/70A; Abcam); anti-mouse NKp46 (M20; Santa Cruz), CD49a (Ha31/8; BD Bioscience), ly6G (1A8; BioLegend), TMEM119 (28-3; Abcam), CD68 (ED1; Abcam), CD8 (53-6.7; eBioscience), CD4 (GK1.5; eBioscience), CD19 (1D3; BD Bioscience), CD31 (polyclonal; Abcam), claudin5 (4C3C2; Invitrogen), and ZO-1 (ZO-1-1A12; Invitrogen). The following fluorochrome-conjugated secondary antibodies were used: donkey anti-rabbit 488 (1:1,000; Invitrogen), donkey anti-rabbit 546 (1:1,000; Invitrogen), donkey anti-goat 546 (1:1,000; Invitrogen), donkey anti-mouse 594 (1:1,000; Invitrogen), donkey anti-mouse 488 (1:1,000; Invitrogen), goat anti-rat 488 (1:1,000; Invitrogen), and goat anti-rat 555 (1:1,000; Invitrogen). Images were acquired on a fluorescence microscope (Olympus BX-61).

Li Z., Li M., Shi S.X., Yao N., Cheng X., Guo A., Zhu Z., Zhang X, & Liu Q. (2020). Brain transforms natural killer cells that exacerbate brain edema after intracerebral hemorrhage. The Journal of Experimental Medicine, 217(12), e20200213.

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