DAPI (dilution 1:1000, Thermo Fisher Scientific, 62248), Goat anti-Hamster IgG Alexa Fluor 647 (dilution 1:200, Jackson ImmunoResearch, 127-605-160), Goat anti-Rabbit IgG Alexa Fluor 430 (dilution 1:200, Thermo Fisher Scientific, A11064), Goat anti-Rabbit IgG Alexa Fluor 430 (dilution 1:200, Thermo Fisher Scientific, A11064), Goat anti-Rabbit IgG Alexa Fluor 594 (dilution 1:200, Thermo Fisher Scientific, A11037), Goat anti-Mouse IgG1 Alexa Fluor 488 (dilution 1:200, Thermo Fisher Scientific, A21121), Goat anti-Mouse IgG1 Alexa Fluor 647 (dilution 1:200, Thermo Fisher Scientific, A21240), Goat anti-Mouse IgG2a Alexa Fluor 546 (dilution 1:200, Thermo Fisher Scientific, A21133), Goat anti-Mouse IgG2b Alexa Fluor 647 (dilution 1:200, Thermo Fisher Scientific, A21242), Goat anti-Guinea Pig IgG Alexa Fluor 546 (dilution 1:200, Thermo Fisher Scientific, A11074), Goat anti-Guinea Pig IgG Alexa Fluor 594 (dilution 1:200, Thermo Fisher Scientific, A11076), Donkey anti-Chicken IgG IRDye 680LT (dilution 1:200, Li-Cor Biosciences, 926-68028), Donkey anti-Rabbit IgG IRDye 800CW (dilution 1:200, Li-Cor Biosciences, 926-32213), Donkey anti-Chicken IgG Alexa Fluor 488 (dilution 1:500, Jackson ImmuoResearch, 703-545-155), and Donkey anti-Guinea Pig IgG Alexa Fluor 647 (dilution 1:500, Jackson ImmuoResearch, 706-605-148).
Matson K.J., Russ D.E., Kathe C., Hua I., Maric D., Ding Y., Krynitsky J., Pursley R., Sathyamurthy A., Squair J.W., Levi B.P., Courtine G, & Levine A.J. (2022). Single cell atlas of spinal cord injury in mice reveals a pro-regenerative signature in spinocerebellar neurons. Nature Communications, 13, 5628.
+ Open protocol