Peroxide free arachidonic acid

11-oxoeicosatetranoic acid (11-oxo-ETE), 15-oxoeicosatetranoic acid (15-oxo-ETE), 11-oxo-eicosatetranoic acid methyl ester (11-oxo-ETE-ME), 15-oxo-eicosatetranoic acid methyl ester (15-oxo-ETE-ME) and the [¹³C₂₀]-labeled 15-oxo-ETE internal standard were synthesized in-house as previously reported (2 (link)). [¹³C₃¹⁵N₁]-Arachidonoyl-CoA used as an internal standard was synthesized as previously reported (19 (link)). Peroxide-free arachidonic acid (AA) was purchased from Cayman Chemical (Ann Arbor, MI). 5-sulfosalicilic acid (SSA), ammonium formate, glacial acetic acid, 2-mercaptoethanol (BME), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). HPLC grade chloroform as well as Optima LC-MS grade methanol, acetonitrile, water, isopropanol, ammonium acetate, and formic acid were purchased from Fisher Scientific (Pittsburgh, PA). 2-(2-pyridyl) ethyl functionalized silica gel (100 mg/mL) solid phase extraction (SPE) cartridges were obtained from Supelco Analytical (Bellefonte, PA). Streptomycin and penicillin were purchased from Invitrogen (Carlsbad, CA). Fetal bovine serum (FBS) was from Gemini Bioproducts (West Sacramento, CA). Human colorectal adenocarcinoma cells (LoVo and HCA-7) were obtained from ATCC (Manassas, VA).

Arachidonoyl-CoA, 5-sulfosalicilic acid (SSA), ammonium formate solution, glacial acetic acid, and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Optima LC-MS grade methanol, acetonitrile (ACN), 2-propanol (IPA) and water were purchased from Fisher Scientific (Pittsburgh, PA). 2-(2-pyridyl) ethyl functionalized silica gel 100 mg/1 mL tubes were obtained from Supelco Analytical (Bellefonte, PA). F-12K media, DMEM, medium 200, low serum growth supplement (LSGS) kit, streptomycin, and penicillin were purchased from Invitrogen (Carlsbad, CA). Charcoal stripped fetal bovine serum (csFBS) was from Gemini Bioproducts (West Sacramento, CA). [¹³C₃¹⁵N₁] calcium pantothenate was purchased from Isosciences (King of Prussia, PA). Peroxide free arachidonic acid (AA) was purchased from Cayman Chemical (Ann Arbor, MI). Human colorectal adenocarcinoma (LoVo) cells and human colon adenocarcinoma (HCA7) cells were obtained from ATCC (Manassas, VA). Livers from C57BL/6 mice were taken under University of Pennsylvania IACUC protocol # 803875.