Lps from e coli o55 b5

Manufactured by Merck Group

Sourced in United States, Germany, China

LPS from E. coli O55:B5 is a lipopolysaccharide extracted from the cell wall of the Escherichia coli O55:B5 strain. It is a commonly used endotoxin for various research applications.

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Lab products found in correlation

Easysep direct human neutrophil isolation kit, by STEMCELL (3 mentions) Aim 5 medium, by Thermo Fisher Scientific (3 mentions) Lps from e coli o111 b4, by Merck Group (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Cd 1 mice, by Charles River Laboratories (1 mentions) Anased, by Akorn (1 mentions) Thioglycolate, by Merck Group (1 mentions) Recombinant mouse tnf α, by R&D Systems (1 mentions) Bay11 7082, by InvivoGen (1 mentions) Palmitic acid, by Merck Group (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Ifn γ, by R&D Systems (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Pd98059, by Merck Group (1 mentions) Human insulin, by Eli Lilly (1 mentions) Il 1β, by Nanjing Jiancheng (1 mentions)

34 protocols using lps from e coli o55 b5

BEND cell culture and LPS treatment

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BEND cells were purchased from the American Type Culture Collection (ATCC® CRL-2398™) and cultured in Dulbecco's modified Eagle's medium (DMEM)/F-12 (HyClone, USA) with 10% FBS (fetal bovine serum, PAN, Germany). 293T cells were kindly provided by Dr. Chenyang Yi (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University) and maintained in RPMI 1640 (Invitrogen, USA) with 10% FBS (PAN, Germany). BEND cells were treated with LPS (from E. coli O55:B5, Merck, Germany) alone or with other corresponding treatments. After the treatments, the cells were prepared for further studies.

Zhao G., Jiang K., Yang Y., Zhang T., Wu H., Shaukat A., Qiu C, & Deng G. (2018). The Potential Therapeutic Role of miR-223 in Bovine Endometritis by Targeting the NLRP3 Inflammasome. Frontiers in Immunology, 9, 1916.

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Cell Culture and Exosome Treatment

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BEND cells were purchased from the American Type Culture Collection (ATCC^® CRL-2398™) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (HyClone, USA) with 10% fetal bovine serum (FBS, PAN, Germany). 293T cells were kindly provided by Dr. Chenyang Yi (State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University) and maintained in Roswell Park Memorial Institute 1640 (RPMI 1640) (Invitrogen, USA) with 10% FBS (PAN, Germany). Cells for exosome treatment were cultured in DMEM/F12 with 10% exosome-depleted FBS (System Biosciences, USA). BEND cells were treated with LPS (from E.coli O55:B5, Merck, Germany) alone or in combination with exosomes or other corresponding treatment. After the treatments, the cells were prepared for further studies.

Zhao G., Yang C., Yang J., Liu P., Jiang K., Shaukat A., Wu H, & Deng G. (2018). Placental exosome-mediated Bta-miR-499-Lin28B/let-7 axis regulates inflammatory bias during early pregnancy. Cell Death & Disease, 9(6), 704.

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LPS-induced Preterm Birth in Mice

Cited 1 time

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All animal care and treatment procedures were approved by the Animal Care and Use Committee of the Johns Hopkins University. Timed pregnant CD-1^® mice were obtained from Charles River Laboratories (Wilmington, MA, USA), and an established model of IUI was used as previously described.^{12 (link),20 (link)–25 (link)} Briefly, on embryonic day (E)17, dams received 25 μg LPS (from E. coli O55:B5; Sigma-Aldrich, St. Louis, MO, USA) in 100 μL phosphate buffered saline (PBS) (pH 7.4, Gibco, ThermoFischer Scientific, Waltham, MA, USA) or 100 μL PBS vehicle via intrauterine injection between the first and second gestational sacs of the right uterine horn. A total of 92 dams were randomly assigned to five treatment groups: PBS, LPS, CD8⁺ T cell depletion (DEP) + PBS, DEP + LPS, and nonsurgical control. Preterm birth was defined as delivery of the litter before E18.5.

Lei J., Xie L., Zhao H., Gard C., Clemens J.L., McLane M.W., Feller M.C., Ozen M., Novak C., Alshehri W., Alhejaily N., Shabi Y., Rosenzweig J.M., Facciabene A, & Burd I. (2017). Maternal CD8+ T cell depletion alleviates intrauterine inflammation-induced perinatal brain injury. American journal of reproductive immunology (New York, N.Y. : 1989), 79(5), e12798.

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Equine Radiocarpal Synovitis Model

Cited 1 time

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The LPS-induced radiocarpal synovitis was selected because it has long been used as an inflammatory pain model in analgesic studies in horses (Palmer & Bertone, 1994 (link); Owens et al., 1996 (link); Todhunter et al., 1996 (link); Smith et al., 1998 (link); de Grauw et al., 2009 (link); Santos et al., 2009 (link); Lindegaard et al., 2010b (link); van Loon et al., 2010 (link)) and because intraplantar LPS was used in several of rodent pain models designed to study the role of sEH inhibitors in anti-nociception (Inceoglu et al., 2006 (link); Schmelzer et al., 2006 (link); Inceoglu et al., 2008 (link); Liu et al., 2010 (link)). Horses were sedated with 0.2–0.5 mg/kg xylazine (AnaSed, Akorn, Inc., IL, USA) i.v. and the skin over both carpi was surgically scrubbed using povidone-iodine antiseptic soap. One radiocarpal joint was injected with 3 µg of lipopolysaccharide (LPS) from E. coli O55:B5 (catalog number L5418, Sigma-Aldrich, St. Louis, MO, USA) (Lindegaard et al., 2010b (link)) freshly prepared sterile to 1.5 µg/mL in 0.9% NaCl. The first injected joint was randomly assigned; subsequent injections alternated between joints. During each experiment, the contralateral joint was not injected but arthrocenthesis was performed for SF collection.

Guedes A.G., Aristizabal F., Sole A., Adedeji A., Brosnan R., Knych H., Yang J., Hwang S.H., Morisseau C, & Hammock B.D. (2017). Pharmacokinetics and anti-nociceptive effects of the soluble epoxide hydrolase inhibitor t-TUCB in horses with experimentally induced radiocarpal synovitis. Journal of veterinary pharmacology and therapeutics, 41(2), 230-238.

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Inflammatory Mediator Modulation Protocol

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ILG was purchased from AK Scientific Inc. (Union City, CA, USA) and Sigma-Aldrich (St. Louis, MO, USA). Pioglitazone was purchased from Funakoshi (Tokyo, Japan). ILG and pioglitazone were dissolved in dimethyl sulfoxide (DMSO). Recombinant mouse TNF-α, IL-4, and IFN-γ were purchased from R & D Systems (Minneapolis, MN, USA). Human insulin was purchased from Eli Lilly (Indianapolis, IN, USA). LPS from E. coli O55:B5, lipid A from Salmonella minnesota, palmitic acid, TDM, ATP, and thioglycolate were purchased from Sigma-Aldrich. palmitic acid was conjugated to bovine serum albumin (BSA, Sigma-Aldrich) to increase solubility. BAY11-7082 was purchased from InvivoGen (San Diego, CA, USA). PD98059 was purchased from Merck Millipore (Darmstadt, Germany).

Watanabe Y., Nagai Y., Honda H., Okamoto N., Yamamoto S., Hamashima T., Ishii Y., Tanaka M., Suganami T., Sasahara M., Miyake K, & Takatsu K. (2016). Isoliquiritigenin Attenuates Adipose Tissue Inflammation in vitro and Adipose Tissue Fibrosis through Inhibition of Innate Immune Responses in Mice. Scientific Reports, 6, 23097.

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Modulation of LPS-induced Endotoxemia

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Balb/c mice were bred in the animal facility at the Molecular Biotechnology Center, University of Turin. LPS from E. coli O55:B5 (25 mg/kg, Sigma) was injected intraperitoneally (i.p.) in male mice aged 6–8 weeks. rNAPRT was injected i.p. at concentration of 1 or 25 mg/kg per mouse alone or 1–2 h before LPS treatment. Mice were closely monitored periodically to detect signs of endotoxemia and survival rate.

Managò A., Audrito V., Mazzola F., Sorci L., Gaudino F., Gizzi K., Vitale N., Incarnato D., Minazzato G., Ianniello A., Varriale A., D’Auria S., Mengozzi G., Politano G., Oliviero S., Raffaelli N, & Deaglio S. (2019). Extracellular nicotinate phosphoribosyltransferase binds Toll like receptor 4 and mediates inflammation. Nature Communications, 10, 4116.

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LPS-induced Lung Inflammation in Mice

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Mice were anesthetized with 100 mg/kg ketamine hydrochloride i.p. and 10 mg/kg xylazine i.p. After anesthesia, mouse tracheas were surgically exposed (Li et al., 2009 (link), 2011 (link)), and a total volume of 50 µl LPS (from E. coli o55:B5; Sigma-Aldrich) per mouse was instilled intratracheally via an angiocatheter that was inserted through the trachea and into the left bronchus. To indicate correct deposition, 1% colloidal carbon was included in the instillate. Upon completion of the experiment, mice were euthanized by CO₂.

Bajrami B., Zhu H., Kwak H.J., Mondal S., Hou Q., Geng G., Karatepe K., Zhang Y.C., Nombela-Arrieta C., Park S.Y., Loison F., Sakai J., Xu Y., Silberstein L.E, & Luo H.R. (2016). G-CSF maintains controlled neutrophil mobilization during acute inflammation by negatively regulating CXCR2 signaling. The Journal of Experimental Medicine, 213(10), 1999-2018.

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Determination of Danofloxacin Levels

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The LPS from E. coli O55:B5 (L2880) was bought from Sigma Aldrich Chemical Co. (St. Louis, MO). The analytical standard for danofloxacin mesylate (Lot No. h0201210) with a purity of 94.2% was supplied by the China Institute of Veterinary Drugs Control (Beijing, China). The raw material of danofloxacin mesylate (Lot No. 201217-1) with a purity of 95.37% was donated by Zhejiang Guobang Pharmaceutical Co., Ltd. (Hangzhou, China). The Enzyme-linked immunosorbent assay (ELISA) kit of chicken corticosterone (CORT) and interleukin-1β (IL-1β) were obtained from Nanjing Jiancheng Institute of Bioengineering (Nanjing, China). Acetonitrile and methanol were of chromatographic grade and purchased from the Shanghai Linen Technology Development Co., Ltd (Shanghai, China). Phosphoric acid and triethylamine were domestic analytical reagents purchased from Tianjin Deen Chemical Reagent Co., Ltd. (Tianjin, China) and Tianjin Kermel Chemical Reagent Co., Ltd (Tianjin, China), respectively.

Wang H., Yang F., Song Z.W., Shao H.T., Bai D.Y., Ma Y.B., Kong T, & Yang F. (2021). The influence of immune stress induced by Escherichia coli lipopolysaccharide on the pharmacokinetics of danofloxacin in broilers. Poultry Science, 101(3), 101629.

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LPS-induced Inflammation in Mice

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Eight-week-old male C57BL/6NCrSlc (B6) mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan). Mice received intraperitoneal injection of 3 mg/kg LPS from E. coli O55:B5 (Sigma, St. Louis, MO, USA) that was dissolved in physiological saline at a total volume of 7.5 mL/kg. As the control, mice received intraperitoneal injection of 7.5 mL/kg physiological saline. For the real-time RT-PCR, mice were examined at 4 h after injection of LPS (n = 4) or saline (n = 4). For the double immunohistofluorescence staining, they were examined at 1 (n = 12), 4 (n = 13), and 24 h (n = 8) after LPS injection, and at 1 h after saline injection (n = 15). All mice were handled in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8023, revised 1978). All experiments were approved by the Institutional Animal Care and Use Committee of the Kyorin University Faculty of Health Sciences (Protocol “I17-08-02”)

Shimada A, & Hasegawa-Ishii S. (2021). Increased cytokine expression in the choroid plexus stroma and epithelium in response to endotoxin-induced systemic inflammation in mice. Toxicology Reports, 8, 520-528.

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Evaluation of Human Cell Chimerism in Sepsis Murine Models

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Because of the variability in the level of human cell chimerism after the transplantation of CD34⁺ cells, the injected mice were pair-matched before the proper experiments. Seven weeks after transplantation, 50 μl of the peripheral blood was collected from the tail vein and placed in the EDTA-containing tubes. Next, the total cell count was evaluated (by using Bürker chamber and Türk’s solution) and the percentage of human CD45⁺ cells was analyzed by flow cytometry after staining with anti-CD45 PE antibody (BD Biosciences). Animals with chimerism varying less that ±2 % were matched into pairs and subjected to experimental endotoxemia or CLP models. Eight weeks after the transplantation, the selected paired mice were subjected to endotoxemia, receiving an intravenous injection of 40 μg of LPS from E. coli O55:B5 (Sigma-Aldrich) in 100 μl of 0.9 % saline or 100 μl of saline as control.

Skirecki T., Kawiak J., Machaj E., Pojda Z., Wasilewska D., Czubak J, & Hoser G. (2015). Early severe impairment of hematopoietic stem and progenitor cells from the bone marrow caused by CLP sepsis and endotoxemia in a humanized mice model. Stem Cell Research & Therapy, 6(1), 142.

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