ChIP assay protocols were previously detailed [22 (link)]. In brief, cell lysates were sonicated using a Misonix Sonicator 3000 Homogenizer, resulting in fragmented genomic DNA. These lysates, diluted in ChIP dilution buffer, were subjected to immunoprecipitation with an anti-GATA4 antibody (Abcam). The DNA bound by GATA4 was eluted from protein A/G agarose, and NaCl was added to reverse cross-linking between proteins and genomic DNA. The DNA containing the proposed conserved Gαi3 promoter site [51 (link)] was subsequently analyzed via quantitative PCR (qPCR).
Sonicator 3000 homogenizer
Manufactured by Bioventus
The Sonicator 3000 Homogenizer is a laboratory instrument designed for the disruption and homogenization of biological samples. It utilizes high-frequency sound waves to break down and mix the contents of the sample, enabling efficient sample preparation for various analytical and experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sonicator 3000 homogenizer
1
ChIP Assay for GATA4 Binding
2
Chromatin Immunoprecipitation (ChIP) Assay for GATA4 Binding
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The detailed protocols of ChIP assay were described previously (53 (link)). In brief, cell lysates were treated with the Misonix Sonicator 3000 Homogenizer (54 (link)), and fragmented genomic DNA was obtained. Lysates diluted with ChIP dilution buffer were immunoprecipitated with an anti-GATA4 (Abcam) antibody. GATA4-bound DNA was eluted from the protein A/G agarose, and NaCl was added to eliminate cross-linking between proteins and genomic DNA. DNA containing the proposed conserved Gαi3 promoter site I (33 (link)) was analyzed via quantitative PCR (qPCR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!