Propofol diprivan

Lipopolysaccharide (LPS), Evans blue, methylcellulose, 70 kDa fluorescein isothiocyanate conjugated dextran were bought from Sigma-Aldrich (St Louis, MO, USA). Propofol (Diprivan®, AstraZeneca, London, British), dexmedetomidine (Yangtze River Pharmaceutical Group, Taizhou, Jiangsu, China), isoflurane (RWD Life Science, Shenzhen, Guangdong, China) and 10% fat emulsion (Intralipid®, Fresenius Kabi, Wuxi, Jiangsu, China) were used in present study.

All drugs were prepared just before use: propofol (Diprivan; AstraZeneca UK limited, Italy: jc393, 20 mL: 200 mg); 20% intralipid (2B6061; Baxter, Deerfield, IL, USA); SAHA (Selleck Chemicals LLC, Houston, TX, USA). HGN antisense was synthesized by Sangon Biotech (Shanghai, China) Co., Ltd. Senegenin (purity ≥ 98%) was purchased from Nanjing SenBeiJia Biological Technology Co., Ltd. (Jiangsu province, China).
Anti‐β‐actin and anti‐rabbit IgG secondary antibody were obtained from Cell Signaling Technology (Cell Signaling Tech, MA, USA). Anti‐CREB (Phospho S133), anti‐NMDAR2B, anti‐HDAC2, antisynaptophysin, anti‐Ac‐H4K12 and anti‐Ac‐H3K14 antibodies were purchased from Abcam (Abcam, Cambridge, MA, USA). Anti‐HGN antibody was synthesized by Kitgen Bio‐tech Co., Ltd.(Zhejiang province, China).

Wild-type C57BL/6N mice or Nestin-GFP mice at postnatal day (PND) 7 were randomly allocated to different groups and administrated 4 mg.kg⁻¹ (L-Propofol group) and 50 mg.kg⁻¹ (H-Propofol group) Propofol (Diprivan; AstraZeneca; Cambridge, England) by i.p. injection. EdU (5-ethynyl-2'-deoxyuridine; A10044; Invitrogen; California, USA) was first given i.p. 30 min after drug injection at a dose of 50 mg kg⁻¹ in 0.9% saline and the same dosage was administrated per 12 h for six times within 3 days.