Rabbit anti transferrin receptor

The following primary antibodies were used for indirect immunofluorescence staining: mouse anti-E-cadherin (1:250, clone 36, BD Biosciences) was used for immunofluorescence staining in Figs 1, 2a and 3 and, Supplementary Figs 1, 4 and 7, rat anti-E-cadherin (1:250, DECMA-1 from Sigma Aldrich was used for immunofluorescence staining in Fig. 2b and Supplementary Fig. 7, rabbit anti-β-catenin (1:400, Abcam), mouse anti-β-catenin (clone 14, 1:250, BD Biosciences), rabbit anti-Transferrin Receptor (1:100, Abcam), rabbit anti-α-catenin (1:250, BD Sigma), mouse anti-α-tubulin (1:500, Sigma Aldrich). The following secondary antibodies were used for indirect immunofluorescence staining: Alexa Fluor 488 conjugated goat anti-rabbit (1:500, Life Technologies), Cy5 conjugated goat anti-rabbit (1:500, Life Technologies), Cy3 conjugated goat anti-mouse (1:500, Life Technologies), CF640 conjugated goat anti-rat (1:500, Life Technologies). Additionally, for staining F-actin either Alex Fluor 488 or Rhodamine conjugated Phalloidin (1:150, Life Technologies) was used.