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3 protocols using ferrous chloride

1

Antioxidant Activity Assessment Protocol

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Folin-Ciocalteau’s phenol reagent (FCR), 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radicals, gallic acid, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), β-carotene, linoleic acid, Tween 40, HPLC standards, including caffeic acid, p-coumaric acid, orientin (luteolin 8-C-β-d-glucoside), isoorientin (luteolin 6-C-β-d-glucoside), vitexin (apigenin 8-C-glucoside), isovitexin (apigenin 6-C-glucoside), vicenin-2 (apigenin 6,8-di-C-β-d-glucoside), and schaftoside (apigenin 8-C-α-l-arabinoside 6-C-β-d-glucoside) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium persulfate, ferrous chloride, and the solvents were provided by Avantor Performance Materials (Gliwice, Poland).
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2

Antioxidant Activity Assay Protocol

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α,α-Diphenyl-β-picrylhydrazyl free radical (DPPH), linoleic acid, butylated hydroxyanisole (BHA), butylated hydroxytoluene, α-tocopherol, bovine serum albumin, thiobarbituric acid, ferrozine, lecithin, SDS (sodium dodecyl sulfate), ammonium thiocyanate, ferric chloride, KH2PO4, and K2HPO4 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium dihydrogen phosphate, disodium hydrogen phosphate, NaBr, and trichloacetic acid were obtained from Merck & Co. Inc. (Kenilworth, JN, USA). Tween 20 was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). HCl, NaCl, and copper sulfate were purchased from the Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). EDTA was purchased from Mallinckrodt Pharmaceuticals (Raleigh, NC, USA). Ferrous chloride, Coomassie brilliant blue G-250, n-butanol, and phosphotungstic acid were bought from Avantor Performance Materials (Baker analyzed reagents; Center Valley, PA, USA).
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3

Antioxidant Capacity Assays and Reagents

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The Folin-Ciocalteu phenol reagent (FCR), gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), ferrozine, caffeic acid, quercetin, p-coumaric acid, ferulic acid, rutin, and kaempferol-3-O-rutinoside were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methanol, acetonitrile, sodium carbonate, ferric chloride, ferrous chloride, ferrous sulfate, and potassium persulfate were acquired from Avantor Performance Materials (Gliwice, Poland). The ACL kit was purchased from Analytik Jena (Jena, Germany).
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