Cobas mira

Manufactured by Roche

Sourced in Switzerland, United States, Germany, Italy, United Kingdom, Australia, France, Brazil

The Cobas Mira is a clinical chemistry analyzer designed for routine laboratory testing. It is capable of performing a wide range of immunoassay and chemistry tests to aid in the diagnosis and monitoring of various medical conditions.

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Close spelling variants of this product

Cobas Mira Plus (115 citations) COBAS MIRA Plus Chemistry Analyzer (23 citations) Cobas Mira autoanalyzer (18 citations) Cobas Mira Plus analyzer (14 citations) Cobas Mira Plus Autoanalyzer (12 citations) COBAS MIRA S analyzer (7 citations) COBAS Mira System (7 citations) Cobas-Mira Chemistry Analyzer (6 citations) Cobas Mira analyser (6 citations) Cobas Mira Chemistry System (6 citations)

115 protocols using cobas mira

Antioxidant Biomarker Quantification Protocol

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Plasma levels of superoxide dismutase (SOD) were obtained using SOD Assay Kit-WST (Cayman Chemical, Ann Arbor, MI, USA). Samples were mixed with WST working solution and enzyme working solution. The samples were then incubated for 20 min at 37℃. The absorbencies of the incubated samples were acquired at 450 nm by a plate reader. SOD activity was determined by a Cobas Mira chemistry analyzer (Roche, Basel, Switzerland).
Plasma levels of glutathione peroxidase (GPx) were acquired by a GPx Assay Kit (Cayman Chemical, Ann Arbor, MI, USA). Samples were incubated for 20 min at 37℃. The absorbances of the incubated samples were acquired at 450 nm by a plate reader. The GPx activity was determined using a Cobas Mira chemistry analyzer (Roche, Basel, Switzerland). The plasma samples were incubated with reagent diluent for 5 min at room temperature. The incubated samples were stimulated by Ran-Cell total antioxidant control (Randox, Crumlin, County Antrim, UK), and then analyzed at 340 nm by a plate reader.
Plasma malondialdehyde (MDA) was measured by a BIOXTECHLPO-586 kit (OXIS Health Products, Inc, OR, USA). The plasma samples were incubated with reagent diluent for 5 min at room temperature. The incubated samples were stimulated by Ran-Cell total antioxidant control (Randox, Crumlin, County Antrim, UK), and then analyzed at 340 nm by a plate reader.

Lee S.H., Pekas E.J., Lee S., Headid RJ I.I.I, & Park S.Y. (2020). The Impact of Aspirin Intake on Lactate Dehydrogenase, Arterial Stiffness, and Oxidative Stress During High‐Intensity Exercise: A Pilot Study. Journal of Human Kinetics, 72, 101-113.

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Plasma Clinical Chemistries and Anemia Characterization

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Plasma clinical chemistries were analyzed as previously described [3 ]. Iron, TIBC, and ferritin were analyzed at the Kansas State Veterinary Diagnostic Laboratory by colorimetric analysis on the Roche Cobas Mira (Roche Diagnostics, Indianapolis, Indiana 46250) per the manufacturer’s protocol. Plasma clinical chemistries were directly measured using the Roche Cobas 8000 system (Roche Diagnostics, Indianapolis, Indiana 46250) per the manufacturers’ protocol. Total insulin was analyzed at ARUP Laboratories by ultrafiltration/quantitative chemiluminescent immunoassay on the Siemens ADVIA Centaur Immunoassay system (Siemens Medical Solutions USA, Inc., Malvern, Pennsylvania 19355). Erythrocyte sedimentation rate (ESR) was measured through a technique correlating directly with the Westergren method using the Fisher Healthcare^™ Dispette^™ 2 and reservoirs pre-filled with 0.25mL of 0.9% saline (Thermo Fisher Scientific). Low hemoglobin, the standard definition of anemia, was defined in our study population as animals with hemoglobin at or below the 25^th percentile (< 12.5 g/dl) among all animals at baseline. Low-normal hemoglobin was defined as animals greater than the 25^th and equal to or lower than the 50^th percentile (12.6–13.5 g/dl). These values are consistent with definitions for anemia in humans (< 12–14 g/dl) [19 ].

Venn-Watson S., Baird M., Novick B., Parry C, & Jensen E.D. (2020). Modified fish diet shifted serum metabolome and alleviated chronic anemia in bottlenose dolphins (Tursiops truncatus): Potential role of odd-chain saturated fatty acids. PLoS ONE, 15(4), e0230769.

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Genotyping and Blood Biomarker Analysis Protocol

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VACUETTE^® K3EDTA tubes (2 mL) (Greiner Bio-One International GmbH, Kremsmünster, Austria) were used for LCT C/T_-13910 genotyping. Genomic DNA was extracted from 200 μL EDTA blood with the Nucleic Acid Isolation Kit I on the MagNA Pure Compact Instrument (Roche Diagnostics, Vienna, Austria) according to the manufacturer’s instructions. Genotyping for the LCT C/T_-13910 polymorphism was performed by real-time PCR and melting curve analysis (LCT T-13910C ToolSet^™ [Roche Diagnostics]) on a LightCycler 2.0 Instrument (Roche Diagnostics) [21 (link)].
Fasting blood samples were drawn into VACUETTE^® LH lithium tubes (4 mL) and centrifuged at 2000 x g for 10 minutes. HCY (normal range: 3.2–10.7 μmol/L), FA (normal range 3.1–17.5 ng/mL) and vitamin B12 (normal range: 182–625 pg/mL) measurements were performed by competitive chemiluminescent immunoassays on a Dimension Vista^® 1500 System (Siemens Healthcare GmbH; Vienna, Austria). Creatinine (male: 0.7–1.3 mg/dL; female: 0.55–1.02 mg/dL) was measured using an enzymatic method applied on a Roche Cobas Mira (Roche Diagnostics, Vienna, Austria). The eGFR (normal: >70 mL/min/1.73m2) was calculated applying the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [22 (link)].

Enko D., Meinitzer A., Brandmayr W., Halwachs-Baumann G., Schnedl W.J, & Kriegshäuser G. (2018). Association between increased plasma levels of homocysteine and depression observed in individuals with primary lactose malabsorption. PLoS ONE, 13(8), e0202567.

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Kidney Injury Biomarker Measurement Protocol

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Measurements of plasma creatinine, urea, and magnesium were performed using the Roche/Hitachi modular P800 autoanalyzer (Roche Diagnostics, Mannheim, Germany). Evaluation of plasma potassium was performed using the Roche/Hitachi modular ISE 900 autoanalyzer (Roche Diagnostics, Mannheim, Germany). Acute kidney injury was defined as a doubling of creatinine levels in the plasma, and acute kidney failure was defined as a tripling of creatinine levels in the plasma [9 (link), 10 (link)]. Cystatin C was evaluated by the Roche Cobas Mira instrument (Hoffman-La Roche Ltd., Basel, Switzerland) using a commercial kit, Dako Cytomation cystatin C Immunoparticles Set (DAKO, Hamburg, Germany) based on particle-enhanced immunoturbidimetry.

Berkovitch M., Tsadik R., Kozer E, & Abu-Kishk I. (2014). The Effect of Hyperbaric Oxygen Therapy on Kidneys in a Rat Model. The Scientific World Journal, 2014, 105069.

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Cytokine and CRP Assessment Protocol

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EDTA tubes were used to collect 5 ml of plasma for the cytokines, while 5 ml blood was collected in serum separation tubes for the analysis of serum C-reactive protein concentration. Samples were transported directly to the Centre for Phytochemistry & Pharmacology at Southern Cross University, where the plasma was centrifuged for 10 min at 4°C at 2,500 rpm. Serum was similarly centrifuged after it had been separated for 10 min at room temperature. The plasma and serum were divided into 500 ml aliquots, stored in Eppendorf tubes and frozen at −80°C. At the end of data collection, samples were transferred in one batch on dry ice to the University of Queensland. Plasma samples were analyzed in single using ELISA kits for IL-1β, IL-6, and TNF-α (Quantikine HS®, RnD Systems, Minneapolis, MN) and IL-10 (Quantikine®, RnD Systems) according to the manufacturer's instructions. Serum samples were analyzed in single for high-sensitivity C-reactive protein using a commercial kit (Kamiya Biomedical, Seattle, WA) according to the manufacturer's instructions and an automated spectrophotometer (Cobas MIRA, Roche Diagnostics, Basel, Switzerland).

Bradbury J., Myers S.P., Meyer B., Brooks L., Peake J., Sinclair A.J, & Stough C. (2017). Chronic Psychological Stress Was Not Ameliorated by Omega-3 Eicosapentaenoic Acid (EPA). Frontiers in Pharmacology, 8, 551.

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Quantifying Cell Death by LDH Release

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As a measure of cell death, the content of released LDH was determined at days 4, 6 and 10 in 90 µl medium samples. To each sample was added 10 µl of Tris/NaCl buffer (0.813 M Tris, 2.033 M NaCl; pH 7.2) and samples were stored at −20°C until analysis. The rate of conversion of NADH to NAD⁺ as an indicator of LDH activity was measured by a fully automatic spectrophotometer (Cobas Mira, Hoffmann LaRoche). Twenty µl media samples were added 20 µl pyruvate and 240 µl NADH (both Sigma). The absorbance at 340 nm (37°C) was used as an index of NADH concentration. Before each set of measurements, changes in absorbance of LDH standard solutions (Boehringer Mannheim) were measured for calculation of a standard curve [44] (link).

Krabbe C., Bak S.T., Jensen P., von Linstow C., Martínez Serrano A., Hansen C, & Meyer M. (2014). Influence of Oxygen Tension on Dopaminergic Differentiation of Human Fetal Stem Cells of Midbrain and Forebrain Origin. PLoS ONE, 9(5), e96465.

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Biochemical Analysis of RBC Samples

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RBC samples were undergone centrifugation at 1000 ×g for 10 min at 4 °C, and the supernatant was analyzed to determine biochemical parameters (lactate and glucose) and hemolysis index. We applied a benchtop automated analyzer (Roche’s Cobas Mira chemistry analyzer, Germany) to enzymatically measure lactate (Audit Diagnostics, Glenbio Ireland) and glucose (Darman Faraz Kave, Iran). Before analysis, the instrument was verified using serum controls (Darman Faraz Kave, Iran). The hemolysis amounts were assessed by the absorption spectrum of free hemoglobin at 415, 450, and 700 nm by a spectrophotometer (UNICO UV-2100, USA). The percentage of hemolysis was then calculated according to the other studies.^{18 (link),19 (link)}

Roshanzamir F., Amini-Kafiabad S., Zarif M.N., Arabkhazaeli A, & Mohammadipour M. (2022). The potential effect of leukocyte filtration methods on erythrocyte-derived microvesicles: One step forward. European Journal of Translational Myology, 32(3), 10708.

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Measuring Lung Injury Cytotoxicity

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The level of LDH activity in the BALF of all treatment groups was measured at each time point after exposure to evaluate cytotoxicity as a measure of lung injury. Measurement of LDH activity in the acellular fluid was obtained using a Cobas Mira chemistry analyzer (Roche Diagnostic Systems; Montclair, IN). LDH activity was quantified by detection of the oxidation of lactate coupled to the reduction of nicotinamide adenine dinucleotide at a spectrophotometric setting of 340 nm.

Farris B.Y., Antonini J.M., Fedan J.S., Mercer R.R., Roach K.A., Chen B.T., Schwegler-Berry D., Kashon M.L., Barger M.W, & Roberts J.R. (2017). Pulmonary toxicity following acute coexposures to diesel particulate matter and α-quartz crystalline silica in the Sprague-Dawley rat. Inhalation toxicology, 29(7), 322-339.

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Hepatocyte Viability and LDH Assays

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Hepatocyte viability was investigated following incubation of cells with 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide (XTT) which is metabolically reduced in viable cells by mitochondrial dehydrogenase to a water-soluble formazan product detectable by absorbance readings [16 (link), 17 (link)]. LDH determination from cell culture medium supernatant was performed according to the optimized standard method of the German Society of Clinical Chemistry (Deutsche Gesellschaft für Klinische Chemie, DGKC). Pyruvate was employed as a substrate and NADH decrease was determined photometrically (Cobas Mira, Roche) [18 (link)].

Sauer M., Doß S., Ehler J., Mencke T, & Wagner N.M. (2017). Procalcitonin Impairs Liver Cell Viability and Function In Vitro: A Potential New Mechanism of Liver Dysfunction and Failure during Sepsis?. BioMed Research International, 2017, 6130725.

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Adiponectin Evaluation in Ovarian Oxidative Stress

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Human recombinant adiponectin was from Sigma-Aldrich, USA (SRP4901) and administrated intraperitoneally. Nitrogen oxide (NOx) levels, ferric reducing ability of plasma (FRAP), and the products of advanced protein oxidation (AOPP) were evaluated in whole homogenized ovaries using the Cobas Mira^® automated analyzer (Roche Diagnostics, Basel, Switzerland) as previously described [22 (link)–24 (link)].

Comim F.V., Gutierrez K., Bridi A., Bochi G., Chemeris R., Rigo M.L., Dau A.M., Cezar A.S., Moresco R.N, & Gonçalves P.B. (2016). Effects of Adiponectin Including Reduction of Androstenedione Secretion and Ovarian Oxidative Stress Parameters In Vivo. PLoS ONE, 11(5), e0154453.

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