Left femora (n 5 per group) were fixed in 4 % paraformaldehyde after killing. A high-resolution micro-computed tomography system (InspeXio SMX-90CT, Shimadzu, Kyoto, Japan) was used to scan the proximal region of the femora. The microcomputed tomography (μCT) machine is equipped with a 90-kV scanning voltage, 10-W power, 109-μA current and 9-μm scan thickness. The femora were placed on a holder between the X-ray source and the flat panel detector (inspeXio SMX--90CT; Shimadzu). The scanning angular rotation was 360°, and the angular increment was 1•6°. After scanning the data, reconstruction of three-dimensional data sets with a voxel size of 9 μm was conducted. Both femoral trabecular and cortical bones were analysed using the commercial software provided with the equipment (TRI3D-BON Analysis software; RATOC).
Inspexio smx 90ct
Manufactured by Shimadzu
Sourced in Japan
The InspeXio SMX-90CT is a compact X-ray computed tomography (CT) system designed for the inspection of small samples. It features a microfocus X-ray source and a high-resolution detector, allowing for detailed 3D imaging of internal structures. The system is capable of generating high-quality CT data for various materials and applications.
Automatically generated - may contain errors
Lab products found in correlation
Tri 3d bon, by Ratoc System Engineering (4 mentions)
Tri 3d bon software, by Ratoc System Engineering (2 mentions)
Nightowl 2 lb983, by Berthold Technologies (1 mentions)
D luciferin, by Promega (1 mentions)
Smx 90ct, by Shimadzu (1 mentions)
Tri bone system, by Ratoc System Engineering (1 mentions)
21 protocols using inspexio smx 90ct
1
Femoral Microstructural Analysis via μCT
2
Micro-CT Evaluation of Cancellous Bone
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The micro-CT technique and image processing approach were based on current guidelines and a previous study (Bouxsein et al., 2010 (link)). All specimens were scanned using a micro focus CT instrument (InspeXio SMX-90CT; Shimadzu, Kyoto, Japan) at a 22 µm horizontal grid spacing and slice interval of 90 kV and tube current of 110 mA. Tissue bone mineral density (tBMD) was calibrated from the gray scale linear value using an imaging reference micro-CT phantom. A review of the literature indicated that filtration does not necessarily require software-based beam hardening corrections for imaging of cancellous bone (Waarsing, Day & Weinans, 2004 (link); Meganck et al., 2009 (link)). In preliminary assessments, a cover of only 0.1 mm Cu as an X-ray filter could reduce the induced beam hardening enough to calculate tBMD and structural indices. To assess the volumetric density and microarchitecture of cancellous bone, Bone J software can be used to calculate a number of structural indices (Doube et al., 2010 (link)). The structural indices determined in this study included BV/TV, degree of anisotropy (DA), trabecular thickness (Tb.Th.), trabecular space (Tb.Sp.), structure model index (SMI), and number of connections (connectivity).
3
Olfactory Organ Visualization via diceCT
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The diffusible iodine-based contrast-enhanced computed tomography (diceCT) procedure followed a previous study [31 (link)]. The olfactory organ was fixed in 4% paraformaldehyde in 0.1 M PB (pH 7.4) and stained with an aqueous solution of Lugol’s iodine (I2KI), 1% I2 and 2% KI in deionized water, for several days at room temperature (RT). Specimens were scanned using a microfocus X-ray CT system, inspeXio SMX-90CT (Shimadzu Corporation, Kyoto, Japan). The diceCT data were analyzed and visualized using VGStudio MAX software (System Create, Osaka, Japan).
4
Quantifying Fracture Repair Dynamics in Mice
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Femurs and the surrounding muscle were excised from sacrificed mice at 2, 4, and 6 weeks after fracture generation and treatment and were then stored in 4% paraformaldehyde for 48 h at 4°C. Micro-CT images of whole femurs in PBS were obtained using a microfocus X-ray CT system (inspeXio SMX-90CT; Shimadzu Co., Ltd., Tokyo, Japan) and the following settings: acceleration voltage, 90 kV; current, 110 mA; voxel size, 20 μm/pixel; and matrix size, 1,024 × 1,024. From the obtained images, 10-mm regions of interest (500 slices) in the midfemur were defined. Three-dimensional (3D) image analysis software (Tri-3D-Bon; Ratoc System Engineering Co., Ltd, Tokyo, Japan) was used to measure new bone volume and bone mineral content in the defined regions, as previously described [21 (link)]. For assessing new bone volume and bone mineral content, a hydroxyapatite (HA) calibration curve was generated from the data obtained from phantom images prepared with 200, 300, 400, 500, 600, 700, and 800 mg HA/cm3. The bone mineral content in each sample was determined by comparing the measured densities in the micro-CT images to the HA calibration curve, and new bone was defined using a threshold value of ≥300 mg/cm3.
5
Zebrafish Vertebral Bone Morphology
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Vertebral bone morphology of adult zebrafish was analyzed by micro-computed tomography scans with inspeXio SMX-90CT (SHIMADZU). Three-dimensional reconstruction and videos were generated with ImageJ software.
6
Micro-CT Analysis of Bioengineered Tooth Transplants
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An X-ray analysis was performed on the upper jaws of the mice that received a transplanted bioengineered tooth using a micro-CT device (inspeXio SMX-90CT, Shimadzu, Kyoto, Japan) with exposures at 90 kV and 110 mA.
7
Bone Regeneration Assessment via X-ray CT
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At two important time points of bone regeneration (6 and 12 weeks after transplantation), 15 animals (3 animals of each group) were selected randomly and were sacrificed to get examined by the X-ray CT system (InspeXio SMX-90CT; Shimadzu, Kyoto, Japan; resolution, 105 lm; section-to-section distance, 105 lm) using an InspeXio scanner. Samples were imaged in an arbitrary manner (10 images/each sample) and were analyzed by ImageJ software. Equivalent sites in samples were measured for standardization of analysis.30 (link)
8
Athymic Nude Mouse Xenograft Tumor Model
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Animal use and procedures were approved by Hiroshima University Research Facilities of Laboratory Animal Science. All methods were performed in accordance with the approved guidelines. Five-week-old male athymic nude mice (CLEA Japan, Shizuoka, Japan) were used. Under intraperitoneal anesthesia with 10% pentobarbital sodium (Somnopentyl®: 0.6 mL/kg, Schering-Plough Animal Health, USA), the anesthetized animals were injected with 1.5 × 106 cells into the right hind leg on day 0. For in vivo imaging, the mice were intraperitoneally injected with D -luciferin (150 mg/kg; Promega). Ten minutes later, photon levels from luciferase was counted using NightOWL II LB 983 (Berthold technologies) according to the manufacturer’s instructions. The data were analyzed using IndiGO2 (Berthold Technology). Bone erosion caused by the primary tumor was visualized using inspeXio SMX-90CT (Shimadzu). At the end of the experiment, the primary tumors and lungs were analyzed in vivo by bioluminescent imaging and also checked histologically.
9
Micro-CT Analysis of Femur Bone Union
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Following the sacrifice of mice eight weeks postsurgery, femurs were extracted and fixed in 4% paraformaldehyde for 48 hours at 4°C. The tissue was subsequently moved to PBS and imaged on a microfocus X-ray CT system (inspeXio SMX-90CT; Shimadzu, Tokyo, Japan). Tube voltage, tube current, and voxel size were 90 kV, 100 μA, and 30 × 30 × 30 μm, respectively. 3D imaging software (TRI/3D BON; Ratoc System Engineering Co., Ltd., Tokyo, Japan) was used to generate 3D reconstructed images at a threshold determined based on discriminant analysis. We evaluated bone union at both the bone defect and docking sites. Bone union was defined as the continuity of cortical bone over three of four images in the sagittal and coronal plane at the center of the bone defect site and docking site, respectively. We also calculated the bone volume (BV) and bone mineral content (BMC) of regenerative new bone at the bone defect site in all samples. All parameters were measured in a rectangular region of interest (ROI) that consisted of a 1500 μm length of bone mass indicative of regenerative new bone between the proximal femoral segment and transport segment.
10
Micro-CT Analysis of Bone Neoformation
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The samples were evaluated by a blind and calibrated researcher using high-resolution X-ray micro-CT (SMX-90 CT, ShimadzuCorp., Kyoto, Japan). Measurements were performed using imaging software (ImageJ v.1.54b, National Institutes of Health, Bethesda, MD, USA).
A standard area in the cortical portion of the bone was used to select a region of interest (ROI), and a color threshold was applied to segment different gray values in the images. This sample was used as a model to calculate the gray intensity of the materials in the analysis herein. Based on these, the threshold was set at 150 to 180. Bone area, bone volume, and the percentage of bone in the defects were measured on the stack of images obtained in the analysis.
The 48 femur samples were prepared and scanned via micro-CT (SMX-90 CT, ShimadzuCorp., Kyoto, Japan), with 360° images, and reconstructed on the inspeXioSMX-90CT (Shimadzu Corp., Kyoto, Japan), totaling 300 images per sample. Quantification and evaluation measurements of bone neoformation were performed using the ImageJ software (ImageJ; National Institutes of Health, Bethesda, MD, USA). An intraclass correlation coefficient test was performed on all specimens before analysis.
A standard area in the cortical portion of the bone was used to select a region of interest (ROI), and a color threshold was applied to segment different gray values in the images. This sample was used as a model to calculate the gray intensity of the materials in the analysis herein. Based on these, the threshold was set at 150 to 180. Bone area, bone volume, and the percentage of bone in the defects were measured on the stack of images obtained in the analysis.
The 48 femur samples were prepared and scanned via micro-CT (SMX-90 CT, ShimadzuCorp., Kyoto, Japan), with 360° images, and reconstructed on the inspeXioSMX-90CT (Shimadzu Corp., Kyoto, Japan), totaling 300 images per sample. Quantification and evaluation measurements of bone neoformation were performed using the ImageJ software (ImageJ; National Institutes of Health, Bethesda, MD, USA). An intraclass correlation coefficient test was performed on all specimens before analysis.
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