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Crl 1780

Manufactured by American Type Culture Collection
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The CRL-1780 is a laboratory equipment product offered by American Type Culture Collection. It is a cell line derived from human epithelial cells. The core function of the CRL-1780 is to serve as a standardized and characterized cell culture model for research and experimentation purposes.

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Lab products found in correlation

Ccl 240, by American Type Culture Collection (4 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) D glucose, by Thermo Fisher Scientific (2 mentions) Hepes, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Ccl 81, by American Type Culture Collection (1 mentions) Crl 2019, by American Type Culture Collection (1 mentions) Tib 202, by American Type Culture Collection (1 mentions) Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions) Ccl 1, by American Type Culture Collection (1 mentions) Minimal essential medium nonessential amino acids, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Gemini Bio (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by ScienCell (1 mentions) Arpe 19, by American Type Culture Collection (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions)

6 protocols using crl 1780

1

Culturing Host Cells for Anaplasma, Coxiella, and Chlamydia Infections

Cited 4 times
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Uninfected and A. phagocytophilum NCH-1 strain–infected human promyelocytic HL-60 cells (CCL-240; American Type Culture Collections [ATCC]) and RF/6A rhesus monkey choroidal endothelial cells (CRL-1780; ATCC) were cultured as previously described (Huang et al, 2010a (link)). HeLa human cervical epithelial cells (CCL-2; ATCC) were maintained as described (Justis et al, 2017 (link)). C. burnetii Nine Mile Phase II (NMII; clone 4, RSA439) and mCherry-C. burnetii NMII were purified from Vero cells (African green monkey kidney epithelial cells; CCL-81; ATCC) or acidified citrate cysteine medium-2 (ACCM-2) and stored as described (Cockrell et al, 2008 (link); Beare et al, 2009 (link)). Mouse alveolar macrophages (MH-S; CRL-2019; ATCC) and THP-1 human monocytic cells (TIB-202; ATCC) were maintained as described (Mulye et al, 2018 (link)). THP-1 cells were differentiated into macrophage-like cells by overnight treatment with 200 nM phorbol 12-myrisate 13-acetate (MilliporeSigma). C. trachomatis serovar L2 (LGV 434) was maintained in HeLa cells at 37°C as described (Rucks et al, 2017 (link)). C. pneumoniae AR39 was maintained in HeLa cells at 35°C as described (Ouellette et al, 2016 (link)). Mammalian cell cultures were low passage and confirmed to be mycoplasma free using the Universal Mycoplasma Detection kit (ATCC) or Mycoplasma PCR Detection kit (MilliporeSigma).
Cockburn C.L., Green R.S., Damle S.R., Martin R.K., Ghahrai N.N., Colonne P.M., Fullerton M.S., Conrad D.H., Chalfant C.E., Voth D.E., Rucks E.A., Gilk S.D, & Carlyon J.A. (2019). Functional inhibition of acid sphingomyelinase disrupts infection by intracellular bacterial pathogens. Life Science Alliance, 2(2), e201800292.
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2

Culturing Human Cells for A. phagocytophilum Study

Cited 1 time
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Uninfected and A. phagocytophilum (NCH-1 strain)-infected human promyelocytic HL-60 cells [CCL-240; American Type Culture Collections (ATCC), Manassas, VA] and RF/6A rhesus monkey choroidal endothelial cells (CRL-1780; ATCC) were cultured as described previously (Huang et al., 2010b (link)). HeLa 229 epithelial cells (CCL-1.2; ATCC) were grown in Roswell Park Memorial Institute 1640 (Invitrogen, Carlsbad, CA) supplemented with 10% foetal bovine serum (FBS; Gemini Bio-Products, Sacramento, CA) at 37°C with 5% CO2. HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium with l-glutamine, 4.5 g l−1 of d-glucose and 100 mg l−1 of sodium pyruvate (Invitrogen) supplemented with 10% FBS, 1× minimal essential medium non-essential amino acids (Invitrogen) and 15 mM of HEPES (Affymetrix, Cleveland, OH) at 37°C with 5% CO2. Human neutrophils were isolated from peripheral blood from healthy donors using Polymorphprep (Axis-Shield; Greiner Bio-One, Frickenhausen, Germany) as described previously (Carlyon et al., 2004 (link)). The protocol (HM11407) for obtaining donor blood for the purpose of isolating neutrophils has been reviewed and approved by the Virginia Commonwealth University Institutional Review Board with respect to scientific content and compliance with applicable research and human subjects regulations.
Truchan H.K., VieBrock L., Cockburn C.L., Ojogun N., Griffin B.P., Wijesinghe D.S., Chalfant C.E, & Carlyon J.A. (2015). Anaplasma phagocytophilum Rab10-dependent parasitism of the trans-Golgi network is critical for completion of the infection cycle. Cellular microbiology, 18(2), 260-281.
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3

Cultivation of Choroid-Retinal Endothelial Cells

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RF/6A (Choroid-retinal endothelial cells; CRL-1780™, ATCC, United States) cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco BRL, United States) supplemented with 10% fetal bovine serum (FBS, ScienCell, United States) and antibiotics (100 U/ml penicillin and 100 μg/ml streptomycin; Gibco, United States). All cells were maintained at 37°C in an incubator containing 5% CO2 and 95% air.
Shi L., Han X., Liu C., Li X., Lu S., Jiang Q, & Yao J. (2022). Long Non-Coding RNA PNKY Modulates the Development of Choroidal Neovascularization. Frontiers in Cell and Developmental Biology, 10, 836031.
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4

Evaluating Cell Line Responses to Apoptosis Signaling

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Monkey endothelial cells of choroid-retina (CRL-1780; ATCC™, Manassas, Virginia), porcine aortic endothelial (PAE) cell lines expressing KDR (PAE/KDR) and, PDGFβ-R (PAE/β-R) (27 (link)) and human retinal pigment epithelial cells (ARPE-19, American Type Culture Collection) were cultured in Ham's F-12/DMEM medium supplemented with 10% fetal calf serum (FCS) (Cambrex, East Rutherford, NJ). The following antibodies were used in this study; monoclonal anti TIMP3 antibody from Chemicon International, Inc (Temecula, CA), anti-phosphotyrosine mAb, clone 4G10 and anti-paxillin mAb, clone 5H11 from Upstate Biotechnology Inc (Lake Placid, NY), anti-FAK mAb, clone 77 from Transduction Laboratories (Lexington, KY), anti-Bax (P-19), anti-Bcl2 rabbit polyclonal antibodies and anti-actin (C-11) goat polyclonal antibody from Santa Cruz Biotechnology (Dallas, TX). Growth factor reduced matrigel was purchased from BD Biosciences (Bedford, MA). Z-VAD-FMK (pan-caspase inhibitor), Z-DEVD-FMK(caspase-3 inhibitor II) and Z-IETD-FMK(caspase inhibitor II) were purchased from Calbiochem (Darmstadt, Germany).
Qi J.H, & Anand-Apte B. (2015). Tissue Inhibitor of Metalloproteinase-3 (TIMP3) Promotes Endothelial Apoptosis via a Caspase-Independent Mechanism. Apoptosis : an international journal on programmed cell death, 20(4), 523-534.
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5

Cultivation of Uninfected and A. phagocytophilum-Infected Human and Macaque Cells

Cited 1 time
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Uninfected and A. phagocytophilum NCH-1 strain infected human promyelocytic HL-60 cells (ATCC CCL-240; American Type Culture Collection [ATCC]) were cultured as previously described (30 (link)). Uninfected and infected RF/6A Macaca mulatta choroidal endothelial cells (CRL-1780; ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with l-glutamine, 4.5 g d-glucose and 100 mg sodium pyruvate (Gibco) supplemented with 10% (vol/vol) heat-inactivated fetal bovine serum (FBS) (Gemini Bioproducts), 1X minimal essential medium containing non-essential amino acids (Gibco) and 15 mM HEPES (Gibco) in a humidified incubator at 37°C with 5% atmospheric CO2.
Read C.B., Lind M.C., Chiarelli T.J., Izac J.R., Adcox H.E., Marconi R.T, & Carlyon J.A. (2022). The Obligate Intracellular Bacterial Pathogen Anaplasma phagocytophilum Exploits Host Cell Multivesicular Body Biogenesis for Proliferation and Dissemination. mBio, 13(6), e02961-22.
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6

Culturing Immune and Endothelial Cells

Cited 2 times
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Uninfected and A. phagocytophilum (NCH-1 strain) infected human promyelocytic HL-60 cells, CCL-240; American Type Culture Collections (ATCC, Manassas, VA, USA), RF/6A rhesus monkey choroidal endothelial cells (CRL-1780; ATCC) and HEK-293T cells were cultured as described previously [15 (link),33 (link)].
Truchan H.K., Cockburn C.L., May L.J., VieBrock L, & Carlyon J.A. (2016). Anaplasma phagocytophilum-Occupied Vacuole Interactions with the Host Cell Cytoskeleton. Veterinary Sciences, 3(3), 25.
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