Erk1 2 c 9

Manufactured by Santa Cruz Biotechnology

ERK1/2 (C-9) is a monoclonal antibody that recognizes the extracellular signal-regulated kinase 1/2 (ERK1/2) proteins. ERK1/2 are serine/threonine protein kinases that are involved in the regulation of various cellular processes.

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Lab products found in correlation

Mouse monoclonal anti human prb, by BD (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Tyrosine hydroxylase, by Abcam (1 mentions) Irdye 800cw goat anti rabbit, by LI COR (1 mentions) Irdye 680rd goat anti mouse, by LI COR (1 mentions) Odyssey infrared scanner, by LI COR (1 mentions) Odyssey version 3, by LI COR (1 mentions) P erk thr202 tyr204, by Cell Signaling Technology (1 mentions) Connexin 43, by Santa Cruz Biotechnology (1 mentions) α tubulin dm1a, by Cell Signaling Technology (1 mentions)

2 protocols using erk1 2 c 9

Western Blot Analysis of Cellular Proteins

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Total cell extracts were obtained by lysing the cells directly using 2× SDS-PAGE sample buffer. Western blotting and processing were then performed as described previously [35 (link)]. The following antibodies were used: mouse monoclonal anti-human pRB (BD Pharmingen); rabbit polyclonal anti-MMP2 and MMP9 (Abcam); rabbit monoclonal p21 (12D1), rabbit polyclonal phospho-Akt (Ser473), rabbit monoclonal pan Akt (Cell Signaling), mouse monoclonal anti-p53 (DO-1), mouse monoclonal anti-β-actin, mouse monoclonal anti-SAP97 (2D11) (Dlg), rabbit polyclonal p130 (C-20) and p107 (C-18), mouse monoclonal p-ERK1/2 (12D4), and ERK1/2 (C-9) were from Santa Cruz.
Immunoblots were developed using Clarity™ Western ECL Substrate (Bio-Rad) and images were captured using the ChemiDoc™ Imaging System (Bio-Rad). Protein band intensities were quantified using ImageJ and normalized with the levels of β-actin, which serves as a loading control.

Boon S.S., Chen Z., Li J., Lee K.Y., Cai L., Zhong R, & Chan P.K. (2019). Human papillomavirus type 18 oncoproteins exert their oncogenicity in esophageal and tongue squamous cell carcinoma cell lines distinctly. BMC Cancer, 19, 1211.

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Quantitative Protein Profiling in Cells

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Protein extractions were performed as previously described²⁸ (link)^,²⁹ (link). Primary antibodies p-ERK (Thr202/Tyr204, Cell Signaling, #4370S; 1:1000 dilution), ERK1/2 (C-9, Santa Cruz Biotechnology, SC-514302; 1:200 dilution), tyrosine hydroxylase (Abcam, ab75875; 1:1000 dilution), adiponectin (rabbit polyclonal, home-made), Connexin43 (Santa Cruz Biotechnology, SC-6560-R) and α-tubulin (DM1A, Cell Signaling, #3873S) were used. Protein abundance was detected using one of the following secondary antibodies: goat anti-mouse IRDye 680RD (LI-COR Biosciences, 926-68070), goat anti-rabbit IRDye 800CW (LI-COR Biosciences, 925-32211) at 1:10,000 dilutions. Antibody decorated membranes were then visualized on a LI-COR Odyssey infrared scanner (LI-COR Biosciences). The scanned data were analyzed using Odyssey Version 3.0 software (LI-COR Biosciences).

Zhu Y., Li N., Huang M., Chen X., An Y.A., Li J., Zhao S., Funcke J.B., Cao J., He Z., Zhu Q., Zhang Z., Wang Z.V., Xu L., Williams K.W., Li C., Grove K, & Scherer P.E. (2022). Activating Connexin43 gap junctions primes adipose tissue for therapeutic intervention. Acta Pharmaceutica Sinica. B, 12(7), 3063-3072.

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