PDA (Potato Dextrose Agar), lactophenol-cotton-blue, 1% sodium hypochlorite, Glycerol 15% was obtained from Difco. From Qiagen Purification kit for DNA was obtained, DNA MiniPrep™ kit (Sigma), HiPurA™ PCR product purification kit (HiMedia Laboratories), Penicillin G, dimethyl sulfoxide (DMSO), streptomycin, trypsin-EDTA, 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Propidium iodide (PI), 2′,7′-dichlorofluorescein diacetate, DNase-free RNase, paraformaldehyde, annexin V-FITC Kit, crystal violet, N-acetyl cysteine, doxorubicin and paclitaxel were purchased from Sigma Chemicals Co. (St. Louis, MO). DAPI mounting medium was purchased from Cell signaling technology (CST). Fetal bovine serum (10%) was purchased from Gibco. Antibodies were purchased from different commercial sources: Anti cytochrome c, anti p21, anti bax and anti bcl-2 were procured from Santa Cruz biotechnology incorporation and anti α-tubulin was procured from Sigma Chemical, St. Louis, MO. Secondary antibodies were obtained from CST. Other reagents were of analytical grade and were purchased from local sources.
Dapi mounting medium
Manufactured by Cell Signaling Technology
Sourced in Japan
DAPI mounting medium is a fluorescent dye used for staining and visualizing DNA in biological samples. It binds to the adenine-thymine (A-T) rich regions of DNA, emitting a blue fluorescence when excited by ultraviolet (UV) light. The medium is designed to preserve the fluorescence signal and protect the sample from photobleaching.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Dnase free rnase, by Merck Group (1 mentions)
Dna miniprep kit, by Merck Group (1 mentions)
Hipura pcr product purification kit, by HiMedia (1 mentions)
Annexin 5 fitc kit, by Merck Group (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Trypsin edta, by Merck Group (1 mentions)
Penicillin g, by HiMedia (1 mentions)
Fluorescence microscope, by Olympus (1 mentions)
2 protocols using dapi mounting medium
1
Cytotoxicity Assays and Apoptosis Analysis
2
Immunofluorescence Staining of PC12 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PC12 cells grew on coverslips for 24 h and were then divided into groups for different treatment. The slips covered with treated cells were fixed by 4% paraformaldehyde, followed by supplement of 1% Triton X-100 for permeabilization and 5% serum for blocking. Subsequently, overnight incubation of the slips and primary antibody (IRF4, 1 : 300) at 4°C was carried out. The next day, they were rewarmed at room temperature for 15 min and incubated with fluorescent secondary antibody (KPL 072-03-15-06) for 1.5 h at room temperature. The coverslips were finally mounted utilizing DAPI mounting medium (Cell Signalling #8961) and observed with a fluorescence microscope (OLYMPUS, Japan) in the dark.
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