The human intestinal epithelial cell line, Caco-2 (ATCC, Manassas, VA), was grown in DMEM/F12 and 5% fetal bovine serum (FBS). Cells were cultured on 24-well, 6.5-mm Transwells (0.4 μm polycarbonate) (Corning, Sigma-Aldrich, St. Louis, MO, USA) until transepithelial membrane resistance reached 250 ohms/cm2.
Transwells 0.4 μm polycarbonate
Manufactured by Corning
Sourced in United States
Transwells (0.4 μm polycarbonate) are membrane inserts designed for cell culture applications. The inserts feature a polycarbonate membrane with a pore size of 0.4 μm, allowing for the study of cell monolayer formation and permeability.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using transwells 0.4 μm polycarbonate
1
Caco-2 Transepithelial Resistance Assay
2
Caco-2 Transepithelial Resistance Assay
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The human intestinal epithelial cell line, Caco-2 (ATCC, Manassas, VA), was grown in DMEM/F12 and 5% fetal bovine serum (FBS). Cells were cultured on 24-well, 6.5-mm Transwells (0.4 μm polycarbonate) (Corning, Sigma-Aldrich, St. Louis, MO, USA) until transepithelial membrane resistance reached 250 ohms/cm2.
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