All data were initially analyzed using a 2-way ANOVA with maternal diet and offspring diet as the independent variables. In order to correct for multiple hypothesis testing of gene expression levels, p values were transformed to q values to take account of the false discovery rates using the p.adjust function in
R stats package (R Foundation for Statistical Computing, Vienna, Austria). Data are represented as means ± SEM. Where p values are reported, an alpha level <0.05 was considered statistically significant. All data analysis was conducted using the
R statistical software package version 2.14.1 (R Foundation for Statistical Computing, Vienna, Austria). In all cases, n refers to the number of litters, and n=8 for all groups. Study power was determined based on effect sizes for gene expression differences observed in our previous studies of this model
24 (link).
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