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Denp20

Manufactured by R&D Systems
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DENP20 is a recombinant human protein produced in E. coli. It is a 20 kDa protein that functions as a dendritic cell nuclear protein.

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Lab products found in correlation

Rab1756, by Merck Group (2 mentions) Prism 9, by GraphPad (2 mentions) Microplate reader, by Tecan (2 mentions) Dplg70, by R&D Systems (1 mentions) Rantes ccl5, by R&D Systems (1 mentions)

4 protocols using denp20

1

Quantification of CSF Protein Biomarkers

Cited 3 times
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The following commercially available ELISAs were used to determine CSF protein levels of AGT, ENPP-2 and TRX-1: [AGT (JP27412; TECAN, IBL International GmbH, Europe), ENPP-2 (DENP20; R&D Systems, Europe), TRX-1 (RAB1756; Sigma-Aldrich, Merck KGaA, Germany)]. All ELISAs were performed according to the manufacturers’ protocols with samples diluted 1:200 for AGT, 1:40 for ENPP-2 and 1:2 for TRX-1. NG and NFL were determined by ELISA, as previously described.5 (link),26 (link) TRX-80 was quantified by an in-house ELISA, as previously published.10 (link),27 (link) Samples were tested in duplicate, and the average was considered for the statistical analyses. The calculated intra- and inter-assay coefficients of variation (CV%) were <6 and <15%, respectively, for the overall ELISAs. Samples with duplicate CV above 15% were remeasured or excluded from the analyses. Absorbance was measured with a microplate reader (Tecan Life Sciences, Männedorf, Switzerland). Concentrations were calculated by interpolation from the standard curves using GraphPad Prism 9 software, through a 4PL curve fit.
Daniilidou M., Eroli F., Alanko V., Goikolea J., Latorre-Leal M., Rodriguez-Rodriguez P., Griffiths W.J., Wang Y., Pacciarini M., Brinkmalm A., Zetterberg H., Blennow K., Rosenberg A., Bogdanovic N., Winblad B., Kivipelto M., Ibghi D., Cedazo-Minguez A., Maioli S, & Matton A. (2023). Alzheimer’s disease biomarker profiling in a memory clinic cohort without common comorbidities. Brain Communications, 5(5), fcad228.
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2

Quantification of CSF Protein Biomarkers

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following commercially available ELISAs were used to determine CSF protein levels of AGT, ENPP-2 and TRX-1: [AGT (JP27412; TECAN, IBL International GmbH, Europe), ENPP-2 (DENP20; R&D Systems, Europe), TRX-1 (RAB1756; Sigma-Aldrich, Merck KGaA, Germany)]. All ELISAs were performed according to the manufacturers’ protocols with samples diluted 1:200 for AGT, 1:40 for ENPP-2 and 1:2 for TRX-1. NG and NFL were determined by ELISA, as previously described.5 (link),26 (link) TRX-80 was quantified by an in-house ELISA, as previously published.10 (link),27 (link) Samples were tested in duplicate, and the average was considered for the statistical analyses. The calculated intra- and inter-assay coefficients of variation (CV%) were <6 and <15%, respectively, for the overall ELISAs. Samples with duplicate CV above 15% were remeasured or excluded from the analyses. Absorbance was measured with a microplate reader (Tecan Life Sciences, Männedorf, Switzerland). Concentrations were calculated by interpolation from the standard curves using GraphPad Prism 9 software, through a 4PL curve fit.
Daniilidou M., Eroli F., Alanko V., Goikolea J., Latorre-Leal M., Rodriguez-Rodriguez P., Griffiths W.J., Wang Y., Pacciarini M., Brinkmalm A., Zetterberg H., Blennow K., Rosenberg A., Bogdanovic N., Winblad B., Kivipelto M., Ibghi D., Cedazo-Minguez A., Maioli S, & Matton A. (2023). Alzheimer’s disease biomarker profiling in a memory clinic cohort without common comorbidities. Brain Communications, 5(5), fcad228.
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3

Plasma Biomarker Profiling in Research

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Plasma legumain concentrations were determined by enzyme immunoassay (EIA) with antibodies from R&D Systems (Duoset DY4769), Stillwater, MN. Serum levels of LDL-associated phospholipase A2 (Lp-PLA2) and autotaxin were determined by EIA from R&D systems (DPLG70 and DENP20, respectively). Plasma levels of tumor necrosis factor (TNF) was determined by a custom human cytokine kit, V-plex (cat # K151A0H-1), from Mesoscale Discovery, Rockville, MD. Plasma levels of platelet-factor (PF)4 (Cat# DY795), neutrophil-activating protein (NAP2/CXCL7) (Cat#DY393), regulated on activation, normal T cell expressed and secreted (RANTES/CCL5) (Cat#DY278) and soluble CD40 ligand (CD40L) (Cat#DY617) were measured by EIAs obtained from R&D Systems. The intra-and inter-assay coefficients of variations were <10% for all EIAs. Routine biochemical analysis was performed in clinical routine laboratory at Oslo University Hospital.
Gregersen I., Narverud I., Christensen J.J., Hovland A., Øyri L.K.L., Ueland T., Retterstøl K., Bogsrud M.P., Aukrust P., Halvorsen B, & Holven K.B. (2024). Plasma legumain in familial hypercholesterolemia: associations with statin use and cardiovascular risk markers. Scandinavian journal of clinical and laboratory investigation, 84(1).
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4

HBV Replication Inhibition by ENPP2 Targeting

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HBV replication-competent clone pSM2-HBV harboring a head-to-tail tandem dimer of the HBV genome (GenBank accession number: V01460) was provided by Dr. Hans Will (Heinrich-Pette-Institute, Germany). Small interfering RNAs (siRNAs) targeting ENPP2 were synthesized by Genepharma, Shanghai (sequences are listed in Supplementary Table S1). Secreted HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) from cell culture media were detected by Roche Diagnostics (36461400, 33448500, respectively). Cultrex pathclear basement membrane extract (3432-010-01), an ELISA kit for detection of secreted ENPP2 (DENP20), and ENPP2 inhibitor PF-8380 (4078) were purchased from R&D system, United States. Cell counting kit-8 (CCK-8, 40203ES80) was purchased from Yeasen, China.
Deng W., Chen F., Zhou Z., Huang Y., Lin J., Zhang F., Xiao G., Liu C., Liu C, & Xu L. (2021). Hepatitis B Virus Promotes Hepatocellular Carcinoma Progression Synergistically With Hepatic Stellate Cells via Facilitating the Expression and Secretion of ENPP2. Frontiers in Molecular Biosciences, 8, 745990.
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