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Dfl300 fx camera

Manufactured by Leica
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The Leica DFL300 FX is a high-performance camera designed for laboratory applications. It features a full-frame sensor and advanced imaging capabilities to capture detailed, high-quality images for scientific and research purposes.

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Lab products found in correlation

Dm6000b microscope, by Leica (4 mentions) Application suite advanced fluorescence software, by Leica (4 mentions) Pna fitc, by Vector Laboratories (2 mentions) Igg2c fitc, by Southern Biotech (2 mentions) Superfrost excell slides, by Thermo Fisher Scientific (1 mentions) Application suite x software, by Leica (1 mentions) Oct compound, by Thermo Fisher Scientific (1 mentions) Las x software, by Leica (1 mentions) Cd3 apc, by Thermo Fisher Scientific (1 mentions) B220 pe, by BD (1 mentions)

6 protocols using dfl300 fx camera

1

Immunohistochemical Analysis of Mouse Spleen

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Mouse spleens were embedded in optimal cutting temperature compound and frozen over dry ice. 10-µm sections were cut on a cryostat, mounted on Superfrost Excell slides (Thermo Fisher Scientific), and fixed in −20°C acetone for 20 min. After rehydration in staining buffer (PBS, 1% goat serum, 1% BSA, 0.1% Tween-20), slides were stained with B220-PE (RA3-6B2; BD Biosciences), CD3-APC (500A2; Life Technologies) and PNA-FITC (Vector Laboratories). Images were acquired and analyzed by using a Leica DM6000B microscope at 10× magnification, a Leica DFL300 FX camera, and Leica Application Suite Advanced Fluorescence software. Analysis was performed by using ImageJ software.
Wray-Dutra M.N., Chawla R., Thomas K.R., Seymour B.J., Arkatkar T., Sommer K.M., Khim S., Trapnell C., James R.G, & Rawlings D.J. (2018). Activated CARD11 accelerates germinal center kinetics, promoting mTORC1 and terminal differentiation. The Journal of Experimental Medicine, 215(9), 2445-2461.
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2

Immunofluorescent Staining and Morphometric Analysis of Mouse Kidneys

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Mouse kidneys were frozen in OCT compound (Thermo Fisher Scientific). Five-micrometer sections were fixed in −20°C acetone for 20 min, rehydrated in staining buffer (PBS, 1% goat serum, 1% BSA, and 0.1% Tween 20), and stained with IgG/FITC (Sigma-Aldrich), IgG2c/FITC (SouthernBiotech), or C3/FITC (MP Biomedicals). Images were acquired using a Leica DM6000 B microscope, Leica DFL360 FX camera, and Leica Application Suite X software. For glomerular IC quantification, images were obtained using a constant exposure and scored from 0 to 3 by two independent observers blinded to genotype. Paraffin-embedded sections of kidney were stained with Jones silver methenamine. Images were acquired using a Leica DM6000 B microscope, Leica DFL300 FX camera, and Leica Application Suite X software. Image analyses of glomerular volume and cell counts were conducted using ImageJ.
Du S.W., Jacobs H.M., Arkatkar T., Rawlings D.J, & Jackson S.W. (2018). Integrated B Cell, Toll-like, and BAFF Receptor Signals Promote Autoantibody Production by Transitional B Cells. Journal of immunology (Baltimore, Md. : 1950), 201(11), 3258-3268.
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3

Detecting Glomerular Immune Deposits

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Mouse kidneys were embedded in OCT compound and snap frozen over dry ice. 5μm sections were cut using Cryostat, mounted on slides and fixed in acetone for 20 mins at -200C. After the fixation, the slides were incubated with blocking buffer (PBS, 1% goat serum, 0.1% Tween-20, 1% BSA). The slides were subsequently stained with anti-FITC IgG, IgG2c and C3 in blocking buffer, washed and coverslipped with aqueous mounting media. Images were acquired using Leica microscope, Leica DFL300 FX camera and Leica software LAS X. Location of glomerular immune complex deposits was scored by a pathologist (C.E.A) blinded to genotype.
Arkatkar T., Jacobs H.M., Du S.W., Li Q.Z., Hudkins K.L., Alpers C.E., Rawlings D.J, & Jackson S.W. (2018). TACI deletion protects against progressive murine lupus nephritis induced by BAFF overexpression. Kidney international, 94(4), 728-740.
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4

Immunofluorescence Staining of Splenic Sections

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Immunofluorescence staining of frozen splenic sections was performed, as described [13 (link)]. Images were acquired using a DM6000B microscope, DFL300 FX camera, and Application Suite Advanced Fluorescence software (Leica).
Kolhatkar N.S., Scharping N.E., Sullivan J.M., Jacobs H.M., Schwartz M.A., Khim S., Notarangelo L.D., Thrasher A.J., Rawlings D.J, & Jackson S.W. (2015). B cell-intrinsic TLR7 signals promote depletion of the marginal zone in a murine model of Wiskott-Aldrich syndrome. European journal of immunology, 45(10), 2773-2779.
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5

Immunohistochemistry of Mouse Spleen and Kidney

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Mouse spleens and kidneys were embedded in OCT compound and snap frozen over liquid nitrogen. 10 μm sections were cut on a cryostat, mounted on Superfrost plus slides, and fixed in −20°C acetone for 20 minutes. After rehydration in staining buffer (PBS, 1% goat serum, 1% BSA, 0.1% Tween-20), slides were stained with: B220-PE, CD4-FITC and GL7-APC (spleen); or, IgG-FITC, IgG2c-FITC, or C3-FITC (kidney). Images were acquired using a Leica DM6000B microscope, Leica DFL300 FX camera, and Leica Application Suite Advanced Fluorescence software. For glomerular immune-complex quantification, images were obtained using a constant exposure and scored from 0–3 by two independent observers blinded to genotype.
Jackson S.W., Scharping N.E., Kolhatkar N.S., Khim S., Schwartz M.A., Li Q.Z., Hudkins K.L., Alpers C.E., Liggitt D, & Rawlings D.J. (2014). Opposing impact of B cell intrinsic TLR7 and TLR9 signals on autoantibody repertoire and systemic inflammation. Journal of immunology (Baltimore, Md. : 1950), 192(10), 4525-4532.
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6

Immunofluorescent Analysis of Murine Tissues

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Frozen sections (embedded in OCT) of murine spleen and kidney were fixed in acetone, rehydrated in staining buffer (PBS, 1% goat serum, 1% BSA, 0.1% Tween-20), and stained with: B220-PE (BD Biosciences), CD3-APC (Life Technologies) and PNA-FITC (Vector Labs) (spleen); or IgG2c-FITC (Southern Biotech), or C3-FITC (MP Biomedicals) (kidney). Images were acquired with a Leica DM6000B microscope, Leica DFL300 FX camera, and Leica Application Suite Advanced Fluorescence software. Glomerular immune complexes were quantified by two independent observers blinded to genotype.
Jacobs H.M., Arkatkar T., Du S.W., Scharping N.E., Jonathan W.o.o.d.s., Li Q.Z., Hudkins K.L., Alpers C.E., Rawlings D.J, & Jackson S.W. (2021). TACI haploinsufficiency protects against BAFF-driven humoral autoimmunity in mice. European journal of immunology, 51(9), 2225-2236.
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