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Anti gapdh mouse monoclonal antibody

Manufactured by Abcam
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Anti-GAPDH mouse monoclonal antibody is a laboratory reagent. It is designed to detect the presence of the GAPDH protein in biological samples.

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Lab products found in correlation

Enhanced bca protein kit, by Beyotime (3 mentions) Supersignal west femto trial kit, by Thermo Fisher Scientific (2 mentions) Anti ccnd1 mouse monoclonal antibody, by Proteintech (2 mentions) Anti lef1 rabbit polyclonal antibody, by Proteintech (2 mentions) Quantity one software, by Bio-Rad (2 mentions) Ripa lysis buffer, by Beyotime (2 mentions) Polyclonal rabbit anti wt 1 antibody, by Santa Cruz Biotechnology (2 mentions) Monoclonal mouse anti synaptopodin antibody, by Progen Biotechnik (2 mentions) Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions) Pvdf membrane, by Abcam (2 mentions) Rabbit anti mouse secondary antibody, by Abcam (2 mentions) Pvdf membrane, by Thermo Fisher Scientific (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ecl western blotting kit, by Thermo Fisher Scientific (2 mentions) Hrp conjugated rabbit anti mouse igg, by Cell Signaling Technology (2 mentions) Anti beta catenin, by Abcam (1 mentions) Anti gsk3 beta, by Abcam (1 mentions) Anti p84 rabbit monoclonal antibody, by Merck Group (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions)

35 protocols using anti gapdh mouse monoclonal antibody

1

Protein Expression and Interaction Analysis

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Protein extracts were resolved through SDS-PAGE, detected using anti-FABP7, anti-beta-catenin, anti-CK1 alpha and anti-GSK3 beta (Abcam, Cambridge, MA, USA), anti-HA, anti-E-cadherin, anti-N-cadherin, and anti-vimentin (Cell Signaling, Danvers, MA, USA) antibodies, respectively. Blotted membranes were stripped and re-blotted with an anti-p84 rabbit monoclonal antibody (Sigma, St. Louis, MO, USA) or anti-GAPDH mouse monoclonal antibody (Abcam, Cambridge, MA, USA) as a loading control.
Bai Q., Yang X., Li Q., Chen W., Tian H., Lian R., Liu X., Wang S, & Yang Y. (2022). Metastatic Tumor Cell-Specific FABP7 Promotes NSCLC Metastasis via Inhibiting β-Catenin Degradation. Cells, 11(5), 805.
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2

Automated Western Blot Analysis

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Skin and cell lysates were obtained using RIPA lysis buffer (PPLYGEN, Beijing, China). Protein concentrations were quantified using an enhanced BCA protein assay kit (Beyotime, Shanghai, China). A 7.5 μg protein sample in each well was analyzed using a Wes automated Western blotting system purchased from Protein Simple (Harris, 2015 (link)). Analysis was conducted using an anti-GAPDH mouse monoclonal antibody diluted 1:100 (Abcam, Cambridge, UK) and anti-KIT mouse monoclonal antibody (BBI, Beijing, China) diluted 1:100.
Hu S., Chen Y., Zhao B., Yang N., Chen S., Shen J., Bao G, & Wu X. (2020). KIT is involved in melanocyte proliferation, apoptosis and melanogenesis in the Rex Rabbit. PeerJ, 8, e9402.
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3

SDS-PAGE Analysis of Cardiomyocyte Proteins

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Total protein was acquired from control cardiomyocytes or cardiomyocytes after one week of T3 treatment and subjected to SDS-PAGE. The lanes were loaded with equal amount of protein and were checked by Ponceau S staining. After blocking with milk, the membranes were incubated with anti-p21 mouse monoclonal antibody (Cell Signaling Technology) or anti-GAPDH mouse monoclonal antibody (Abcam) overnight while shaking at 4°C. After incubation with anti-mouse horseradish peroxidase-coupled secondary antibody (Santa Cruz Biotechnology), bands were visualized with SuperSignal West Femto Trial Kit (Thermo Scientific) and quantified using the Quantity One software from BioRad.
Yang X., Rodriguez M., Pabon L., Fischer K.A., Reinecke H., Regnier M., Sniadecki N.J., Ruohola-Baker H, & Murry C.E. (2014). Tri-iodo-L-thyronine Promotes the Maturation of Human Cardiomyocytes-Derived from Induced Pluripotent Stem Cells. Journal of molecular and cellular cardiology, 72, 296-304.
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4

Protein Extraction and Western Blot Analysis

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A cell lysis buffer for Western and IP (Beyotime, Shanghai, China) was used for collecting the total protein. The protein loading amount was 30 μg, and the protein was detected using ChemiDoc™ Touch (Bio-Rad, Hercules, CA, USA). The following antibodies were used: 1:20000 anti-GAPDH mouse monoclonal antibody (Abcam, Cambridge, MA, USA), and 1:1000 anti-HOXC13 mouse monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
Yao F., Zhao B., Hu S., Bai S., Jin R., Zhang C., Chen Y, & Wu X. (2022). miR-129-5p Participates in Hair Follicle Growth by Targeting HOXC13 in Rabbit. Genes, 13(4), 679.
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5

Western Blot Protein Detection Protocol

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Western blot was performed as previously reported [40 (link)]. Primary antibodies were anti-ABHD2 rabbit polyclonal antibody (1:250, Abgent AP13083c, San Diego, USA), anti-phospho ERK1/2 rabbit monoclonal antibody (1:1,000, Cell Signal Technology, 197G2, Danver, JAPAN), anti-ERK1/2 rabbit monoclonal antibody (1:1000, Cell Signal Technology, 137F5), anti-phospho p38 MAPK rabbit monoclonal antibody (1:1000, Cell Signal Technology, (D13E1)XP) anti-p38MAPK rabbit monoclonal antibody (1:1000, Cell Signal Technology, (D3F9)XP) and anti-GAPDH mouse monoclonal antibody (1:1000, Abcam, ab8245, Cambridge, UK). After washing in tris-buffered saline (TBS)-T, the blots were incubated with the appropriate peroxidase-coupled secondary antibody (1:2000; Anti-rabbit HRP or anti-mouse HRP, GE Healthcare Life Science). Specific proteins were detected using ECL Plus Western Blotting Reagent (GE Healthcare Life Science). The bands were visualized using Molecular Imager Gel DocTMXR+ and ChemiDocTMXRS+ Systems with Image Lab 2.0 software (Bio-Rad).
Yamanoi K., Matsumura N., Murphy S.K., Baba T., Abiko K., Hamanishi J., Yamaguchi K., Koshiyama M., Konishi I, & Mandai M. (2016). Suppression of ABHD2, identified through a functional genomics screen, causes anoikis resistance, chemoresistance and poor prognosis in ovarian cancer. Oncotarget, 7(30), 47620-47636.
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6

Rabbit Skin Protein Isolation for Wes Analysis

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Total protein from rabbit skin was isolated using cell lysis buffer with 1% phenylmethanesulfonyl fluoride (PMSF) (Beyotime, Shanghai, China) for Wes simple western analysis. The protein concentration of the lysed cells was estimated using the BCA protein assay kit (Beyotime, Shanghai, China). Protein assay was performed using Wes̛s automated western blotting system (ProteinSimple) (Harris, 2015 (link)). Anti-GAPDH mouse monoclonal antibody (Abcam, Cambridge, United Kingdom), anti-MITF mouse monoclonal antibody (SANTA CRUZ, Dallas, TX, United States), USP13 monoclonal antibody (Proteintech, Wuhan, China) in 1: 100 dilution was used.
Hu S., Bai S., Dai Y., Yang N., Li J., Zhang X., Wang F., Zhao B., Bao G., Chen Y, & Wu X. (2021). Deubiquitination of MITF-M Regulates Melanocytes Proliferation and Apoptosis. Frontiers in Molecular Biosciences, 8, 692724.
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7

Fatty Acid-Induced Signaling Pathways

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Total protein was acquired from control cardiomyocytes or cardiomyocytes after 15 and 30 min of fatty acid treatment and subjected to SDS-PAGE. The lanes were loaded with equal amount of protein and were checked by Ponceau S staining. After blocking with milk, the membranes were incubated with anti-phospho-AMPK (2535S), anti-phospho-ACC (3661S), anti-phospho-Akt (4051S), anti-phospho-ERK (9106S), anti-phospho-p38 MAPK antibodies (9211S) (Cell Signaling Technologies), or anti-GAPDH mouse monoclonal antibody (Abcam, ab8245) overnight while shaking at 4°C. After incubation with anti-mouse (for phospho-Akt, phospho-ERK, and GAPDH) or anti-rabbit (for phospho-AMPK, phospho-p38-MAPK, and phospho-ACC) horseradish peroxidase (HRP)-coupled secondary antibody (Santa Cruz Biotechnology, sc-2031 for goat anti-mouse immunoglobulin G [IgG]-HRP and sc-2004 for goat anti-rabbit IgG-HRP), bands were visualized with SuperSignal West Femto Trial Kit (Thermo Scientific).
Yang X., Rodriguez M.L., Leonard A., Sun L., Fischer K.A., Wang Y., Ritterhoff J., Zhao L., Kolwicz SC J.r., Pabon L., Reinecke H., Sniadecki N.J., Tian R., Ruohola-Baker H., Xu H, & Murry C.E. (2019). Fatty Acids Enhance the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells. Stem Cell Reports, 13(4), 657-668.
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8

Exosome Protein Extraction and Analysis

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Exosome protein was obtained using the RIPA Lysis Buffer (Beyotime, China). The protein concentrations were determined with the Enhanced BCA Protein Kit (Beyotime) [51 (link)]. After SDS polyacrylamide gel electrophoresis and electroblotting, the membranes were incubated with the following primary antibodies: anti-GAPDH mouse monoclonal antibody (Abcam, UK), anti-calnexin monoclonal antibody (Cat#: 66903-1-Ig, Proteintech), anti-Alix monoclonal antibody (Cat#: 12422-1-AP, Proteintech), and anti-TSG101, CD9 rabbit polyclonal antibody (Cat#: 67381-1-Ig, Cat#: 20597-1-AP, Proteintech). HRP-conjugated Affinipure Goat Anti-Rabbit IgG (H+L) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H+L) (Cat#: SA00001-2, Cat#: SA00001-1, Proteintech) were used. Blots were developed using the Tanon ABL X5 Series Intravital Imaging Systems (Tianneng Technology, China) and quantified with ImageJ software.
Li J., Zhao B., Dai Y., Zhang X., Chen Y, & Wu X. (2022). Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022, 9042345.
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9

SARS-CoV-2 Spike Protein Detection

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Chemical and biological reagents were purchased from Sigma-Aldrich/Merck (Milan, Italy), if not-otherwise specified. pcDNA3.1_ spike_del19 was a gift from Raffaele De Francesco (Addgene plasmid # 155297; http://n2t.net/addgene:155297 (accessed on 5 February 2024); RRID:Addgene_155297). The pcDNA3-EGFP was a gift from Doug Golenbock (Addgene plasmid #13031; http://n2t.net/addgene:13031 (accessed on 5 February 2024); RRID:Addgene_13031).
The following antibodies were used throughout this work:

Anti-NC rabbit polyclonal antibody (GTX135357, GeneTex, Irvine, CA, USA), dilution: 1:1000.

Anti-GAPDH mouse monoclonal antibody (ab8245, Abcam, Cambridge, UK), dilution: 1:10,000.

Anti-mouse and anti-rabbit HRP-conjugated antibody (#170-6516 and #170-6515, BioRad, Hercules, CA, USA), dilution: 1:3000.

Anti-S IgG rabbit monoclonal antibody (40592-V05H, Sino Biological, Beijing, China), dilution: 1:200.

Anti-N IgG mouse monoclonal antibody (#33717, Cell Signaling, Danvers, MA, USA), dilution: 1:6400.

Anti-ACE2 IgG rabbit monoclonal antibody (ab15348, Abcam), dilution: 1:200.

αr488: donkey anti-rabbit monoclonal IgG conjugated to AlexaFluor488 (a21206, Thermo Fisher, Milan, Italy). Immunolabeling dilution: 1/400.

Quaranta P., Scabia G., Storti B., Dattilo A., Quintino L., Perrera P., Di Primio C., Costa M., Pistello M., Bizzarri R, & Maffei M. (2024). SARS-CoV-2 Infection Alters the Phenotype and Gene Expression of Adipocytes. International Journal of Molecular Sciences, 25(4), 2086.
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10

Protein Expression Analysis in Skin and Cells

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The skin and cell samples were collected for detecting the protein expression level. Protein lysates were obtained using the RIPA lysis buffer (PPLYGEN, Beijing, China). The protein concentration was measured using the Enhanced BCA Protein Kit (Beyotime, Shanghai, China). The protein samples were diluted to 0.5 μg/μL for detecting the protein level. The protein (1.5 ng) was analyzed using the automated Western blotting system (Protein Simple Wes) [29 ], according to the manufacturer’s instructions. The following antibodies were used: 1:2000 anti-GAPDH mouse monoclonal antibody (Abcam), anti-WNT2 rabbit polyclonal antibody (Bioss, Beijing, China), and 1:100 anti-CCND1 mouse monoclonal antibody, anti-LEF1 rabbit polyclonal antibody, anti-KRTAP11-1 mouse polyclonal antibody, and anti-STAT1 mouse monoclonal antibody (Proteintech, Wuhan, China).
Zhao B., Li J., Liu M., Hu S., Yang N., Liang S., Zhang X., Dai Y., Bao Z., Chen Y, & Wu X. (2022). lncRNA2919 Suppresses Rabbit Dermal Papilla Cell Proliferation via trans-Regulatory Actions. Cells, 11(15), 2443.
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