Imaging was conducted on an Olympus FV1200MPE BASIC (BX-61WI) microscope equipped with a 25×, 1.05 NA water-immersion objective (XLPL25XWMP, Olympus) and an ultrafast pulsed laser (Mai Tai DeepSee HP, Spectra-Physics) tuned to 910 nm. Epifluorescence emission was separated into “green” and “red” channels with a 570nm dichroic mirror and a 525/50 bandpass filter (FF03-525/50-32, Semrock, green channel) and 575-630nm bandpass filter (BA575-630, Olympus, red channel), respectively. The microscope system was controlled by FluoView software (FV10-ASW v.4.1). Images of 256 × 128 pixels representing 256 × 128 μm on the retina were acquired at 15 Hz (zoom setting of 2).
Ff03 525 50 32
Manufactured by IDEX Corporation
The FF03-525/50-32 is a narrow bandpass filter designed for use in laboratory and research applications. It has a center wavelength of 525 nanometers and a bandwidth of 50 nanometers. The filter features a 32-millimeter diameter aperture.
Automatically generated - may contain errors
2 protocols using ff03 525 50 32
1
Two-Photon Imaging of Retinal Specimens
2
Two-Photon Imaging of Retinal Specimens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imaging was conducted on an Olympus FV1200MPE BASIC (BX-61WI) microscope equipped with a 25×, 1.05 NA water-immersion objective (XLPL25XWMP, Olympus) and an ultrafast pulsed laser (Mai Tai DeepSee HP, Spectra-Physics) tuned to 910 nm. Epifluorescence emission was separated into “green” and “red” channels with a 570nm dichroic mirror and a 525/50 bandpass filter (FF03-525/50-32, Semrock, green channel) and 575-630nm bandpass filter (BA575-630, Olympus, red channel), respectively. The microscope system was controlled by FluoView software (FV10-ASW v.4.1). Images of 256 × 128 pixels representing 256 × 128 μm on the retina were acquired at 15 Hz (zoom setting of 2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!