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Application suite version 2.7.1 r1

Manufactured by Leica
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Application Suite version 2.7.1 R1 is a software package designed for Leica lab equipment. The core function of this software is to provide a comprehensive interface for the control and management of Leica's analytical instruments and associated data processing.

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Lab products found in correlation

Bx40 microscope, by Olympus (2 mentions) Dfc420 camera, by Leica (1 mentions) Mp 7714, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Application Suite – Version 1.6.0 Leica Application Suite, version 2.8.1 Leica Application Suite 1.0 software Leica Suite version 2.8.1 Leica Applications Suite Leica Application Suite Leica Application

2 protocols using application suite version 2.7.1 r1

1

Immunohistochemical Analysis of Breast Tissues

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Human tissue arrays were obtained from BioChain (Newark, CA, USA) and contained 18 cases of normal, premalignant, and malignant breast tissues (#Z7020010). DCIS slides were provided by Dr. Rohit Bhargava (Department of Pathology, UPMC, Pittsburgh). Slides were deparaffinized by baking overnight at 59°C. Endogenous peroxidase activity was eliminated by treatment with 30% H2O2 for 15 min at room temperature. Antigen retrieval was performed by microwave heating in 0.1% citrate buffer for 10 min. Non-specific binding sites were blocked with 1% BSA. Reaction with anti-CD68 (1:100), anti-CEACAM1 (1:50) and anti-Annexin A1 (1:100) was performed for 1 hour at room temperature. Secondary antibodies were added at 1:100 dilution for 30 min. Positive signals were visualized by a DAB Substrate Kit (cat. #550880, BD Pharmingen) according to the manufacturer’s protocol. Histology sections were viewed on an Olympus BX40 microscope. Images were acquired using Leica DFC420 camera and Leica Application Suite version 2.7.1 R1.
Jacqueline C., Dracz M., Boothman S., Minden J.S., Gottschalk R.A, & Finn O.J. (2021). Identification of Cell Surface Molecules That Determine the Macrophage Activation Threshold Associated With an Early Stage of Malignant Transformation. Frontiers in Immunology, 12, 749597.
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2

Immunohistochemical Analysis of Inflammation Markers

Cited 1 time
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Slides were deparaffinized by baking overnight at 59°C. Endogenous peroxidase activity was eliminated by treatment with 30% H2O2 for 15 min at room temperature. Antigen retrieval was performed by microwave heating in 0.1% citrate buffer for 10 min at 850V. Nonspecific binding sites were blocked with 2% BSA. Reaction with anti-CD163, anti-CD68, anti-CD86, anti-sTn, anti-ST6GALNAC1 (listed above) was for 16h at 4C. Staining was performed by the avidin-biotin-peroxidase complex method with a commercial kit (PK4000, Vectastain ABC HRP kit; Vector Laboratories) according to the manufacturer’s protocol. Positive signals were visualized by a DAB Substrate Kit (cat. #550880, BD Pharmingen) according to the manufacturer’s protocol. The total inflammation score for each sample was determined as previously described [8 (link)]. For double staining IHC, ImmPRESS duet staining HRP/AP polymer kits, anti-rabbit IgG-brown and anti-mouse IgG red was used (MP-7714, Vector Laboratories) according the manufacturer’s protocol. Histology sections were observed using an Olympus BX40 microscope. Images were acquired using a Leica DFC420 camera and Leica Application Suite version 2.7.1 R1.
Kvorjak M., Ahmed Y., Miller M.L., Sriram R., Coronello C., Hashash J.G., Hartman D.J., Telmer C.A., Miskov-Zivanov N., Finn O.J, & Cascio S. (2019). Crosstalk between colon cells and macrophages increases ST6GALNAC1 and MUC1-sTn expression in ulcerative colitis and colitis–associated colon cancer. Cancer immunology research, 8(2), 167-178.
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