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Bms2017hs

Manufactured by Thermo Fisher Scientific

The BMS2017HS is a laboratory equipment product designed for precise and efficient sample preparation. It features a high-speed centrifugation function to separate samples based on density differences. The product's core function is to facilitate the separation and concentration of various substances within a sample.

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2 protocols using bms2017hs

1

Evaluating Plasma Cytokine Profiles

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The collected serum samples underwent laboratory analyses, which included collection of the following: complete metabolic panel (CMP), complete blood count (CBC), inflammatory markers (c reactive protein [CRP], immunoglobulins A [IgA], G [IgG], and M [IgM]), and cytokine assays. ELISA was used to assess plasma levels of the cytokine IL-22 (BMS2047, Invitrogen) and high-sensitivity ELISA was used to measure plasma IL-17 (BMS2017HS, Invitrogen) per manufacturer’s instructions; plasma was diluted 1:2 for both respective assays. Results were read on a Spectra Max Plus 384 plate reader and analyzed using SoftMax Pro software (Molecular Devices, San Jose, CA). Other cytokines mentioned were measured using Luminex platforms.
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2

Quantifying Inflammatory Markers in Samples

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Levels of IL-17, IL-6; LIGHT; TNF-α and 27-OHC were quantified with human-specific enzyme-linked immunosorbent assay kits according to the manufacturers’ instructions (Cat# BMS2017HS [for IL-17]; Cat# BMS213HS [for IL-6]; Cat# BMS2218 [for LIGHT]; Cat# BMS223HS [for TNF-α] from Invitrogen and LS-F40084 [for 27-OHC] from LSBio).
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