The collected serum samples underwent laboratory analyses, which included collection of the following: complete metabolic panel (CMP), complete blood count (CBC), inflammatory markers (c reactive protein [CRP], immunoglobulins A [IgA], G [IgG], and M [IgM]), and cytokine assays. ELISA was used to assess plasma levels of the cytokine IL-22 (BMS2047, Invitrogen) and high-sensitivity ELISA was used to measure plasma IL-17 (BMS2017HS, Invitrogen) per manufacturer’s instructions; plasma was diluted 1:2 for both respective assays. Results were read on a Spectra Max Plus 384 plate reader and analyzed using SoftMax Pro software (Molecular Devices, San Jose, CA). Other cytokines mentioned were measured using Luminex platforms.
Bms2017hs
Manufactured by Thermo Fisher Scientific
The BMS2017HS is a laboratory equipment product designed for precise and efficient sample preparation. It features a high-speed centrifugation function to separate samples based on density differences. The product's core function is to facilitate the separation and concentration of various substances within a sample.
Automatically generated - may contain errors
2 protocols using bms2017hs
1
Evaluating Plasma Cytokine Profiles
2
Quantifying Inflammatory Markers in Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of IL-17, IL-6; LIGHT; TNF-α and 27-OHC were quantified with human-specific enzyme-linked immunosorbent assay kits according to the manufacturers’ instructions (Cat# BMS2017HS [for IL-17]; Cat# BMS213HS [for IL-6]; Cat# BMS2218 [for LIGHT]; Cat# BMS223HS [for TNF-α] from Invitrogen and LS-F40084 [for 27-OHC] from LSBio).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!