Anti mhc class 2 1 a 1 e m5 114

The following antibodies were used for flow cytometry analysis: anti-IFNγ (XMG1.2, 1:50), anti-CD45.1 (A20, 1:100), anti-CD45.2 (104, 1:100), anti-IL1β-APC (NJTEN3, 1:50), anti-CD44 (IM7, 1:100), anti-CD62l (MEL-14, 1:100), anti-F4/80 (BM8, 1:100), anti-CCR7 (4B12, 1:100), anti-CD4 (RM4-5, 1:200), anti-CD8α (53-6.7, 1:200), anti-H-2K^b (AF6-88.5.5.3, 1:200), anti-Ly6G (RB6-8C5, 1:100), anti-MHC class II (I-A/I-E) (M5/114.15.2, 1:100) and anti-CD11c (N418, 1:100) were from eBioscience. Anti-CD103 (2E7, 1:100) was from Biolegend. Annexin V apoptosis Detection kit (556547) was from BD Pharmingen. For intracellular cytokine staining, Cytofix/Cytoperm plus kit (BD, cat. 55508) was used. For Foxp3 staining, Foxp3 staining buffer set (eBioscience, cat. 00-5523-00) was used. Multiple-colour flow cytometric analysis was performed using FACSAria (BD Biosciences; Franklin Lakes, NJ, USA). For FACS sorting, cells stained with FACS
antibodies were sorted on BD FACSAria device. FlowJo software was used for data acquiring and analysis.

The following antibodies to mouse antigens were used: phycoerythrin (PE) anti-CD45.2 (104; eBioscience), phycoerythrin-indotricarbocyanine (PE-Cy7) anti-CD11c (HL3; BD Bioscience); anti-MHC class II (I-A/I-E) (M5/114.15.2; eBioscience); anti mouse-CD86 (clone GL-1; Biolegend), anti-CD80 (16-10A1, Biolegend); FITC anti-CD40 (HM40-3; BD Bioscience) and Alexa-546 anti-CD207 (929F3.01, Dendritics). Isotype-matched control antibodies were obtained from BD Biosciences. Flow count fluorospheres (Beckman Coulter) were used to determine absolute cell counts.