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Antibodies against nrf2

Manufactured by Proteintech
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Sourced in United States

Proteintech offers high-quality antibodies against Nrf2, a transcription factor that plays a crucial role in the cellular response to oxidative stress. These antibodies are designed for use in various research applications, such as Western blotting, immunohistochemistry, and immunofluorescence, to study the expression and localization of Nrf2 in biological samples.

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Lab products found in correlation

Streptozotocin (stz), by Merck Group (2 mentions) Iron assay kit, by BioAssay Systems (1 mentions) Erastin, by Apexbio (1 mentions) P nrf2, by Proteintech (1 mentions) Ml385, by Apexbio (1 mentions) β actin, by Proteintech (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Metformin hydrochloride tablets, by Bristol-Myers Squibb (1 mentions) Sirt1, by Bioworld Technology (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Lamin b1, by Abcam (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Icam 1, by Santa Cruz Biotechnology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Flag keap1, by Addgene (1 mentions) Quickchange xl mutagenesis kit, by Agilent Technologies (1 mentions) Antibodyagainst flag tag, by Merck Group (1 mentions) Gfp ub, by Addgene (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) High dmem, by Thermo Fisher Scientific (1 mentions)

4 protocols using antibodies against nrf2

1

Ferroptosis Modulators in Oxidative Stress

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Art (purity ≥ 98%), erastin, and ML385 were purchased from APExBIO Technology LLC (Houston, USA). Streptozotocin (STZ) was purchased from Sigma‒Aldrich (USA). Antibodies against Nrf2, p-Nrf2, β-actin, heme oxygenase 1 (HO-1), and GPX4 were from Proteintech Group, Inc. (USA). The Iron Assay Kit was purchased from BioAssay Systems (Hayward, CA, USA). ROS, MDA, and GSH assay kits were from Nanjing Jiancheng Biotechnology Co., Ltd. (Nanjing, China).
Wang B., Zhu S., Guo M., Ma R.D., Tang Y.L., Nie Y.X, & Gu H.F. (2024). Artemisinin ameliorates cognitive decline by inhibiting hippocampal neuronal ferroptosis via Nrf2 activation in T2DM mice. Molecular Medicine, 30, 35.
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2

Antioxidant Mechanisms in Diabetic Nephropathy

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DMEM and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY, United States). PA used for cell treatment was purchased from National Institutes for Food and Drug Control (Guangzhou, China). PA used in animal trial was from Zelang (purity > 98.0%, HPLC; Nanjing, China). Metformin Hydrochloride Tablets used in animal experiments were purchased from Bristol-Myers Squibb Company (Shanghai, China). DMSO and STZ were purchased from Sigma-Aldrich (St. Louis, MO, United States). Primary antibodies against FN (catalog: sc-18825) and ICAM-1 (catalog: sc-1511) were from Santa Cruz Biotechnology (Dallas, TX, United States); Antibodies against Nrf2 (catalog: 16396-1-AP) and SOD-1 (catalog: 10269-1-AP) were purchased from Proteintech Group (Wuhan, China); Sirt1 (catalog: BS6494) was from Bioworld Technology (St. Paul, MN, United States); HO-1 (catalog: A1346) was from Abclonal Technology (Baltimore Avenue, United States); Lamin B1 (catalog: ab-133741) was from Abcam (Cambridge, MA, United States); Anti-rabbit and anti-goat secondary antibodies were purchased from Beyotime (Haimen, China); Alexa Fluor® 488 goat anti-rabbit IgG (catalog: A-11008) was purchased from Molecular Probes (Eugene, OR, United States).
Zhang L., Chen Z., Gong W., Zou Y., Xu F., Chen L, & Huang H. (2018). Paeonol Ameliorates Diabetic Renal Fibrosis Through Promoting the Activation of the Nrf2/ARE Pathway via Up-Regulating Sirt1. Frontiers in Pharmacology, 9, 512.
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3

Characterization of KEAP1 Mutants

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wt KEAP1 is cloned in N-terminally HA- and Strep-Tactin tagged pcDNA5 plasmid (gift from Dr. Markku Varjosalo, University of Helsinki). Site-directed mutagenesis to generate R320Q and R470C mutants from wild type (wt) KEAP1 was performed using Agilent Quick change XL mutagenesis kit. 3xMyc-TRAF2 (#44104), GFP-Ub (#11928), GFP-P65 reporter (#127172) and Flag-KEAP1 (#28023) plasmids were from Addgene. NL3.2.NF-ΚB-RE (#N1111) was from Promega. Antibodies against KEAP1, TRAF2, GFP, MYC and HA were from Proteintech. Antibody against FLAG tag was from Sigma, and antibodies against NRF2 (Proteintech) and ACTIN were from Santa Cruz. Antibodies against S11-pTRAF2, PARP and cleaved PARP were from Cell Signaling. Secondary antibodies with AlexaFluor −680, −555 and −488 labels were from Thermo Scientific.
Deen A.J., Adinolfi S., Härkönen J., Patinen T., Liu X., Laitinen T., Takabe P., Kainulainen K., Pasonen-Seppänen S., Gawriyski L.M., Arasu U.T., Selvarajan I., Mäkinen P., Laitinen H., Kansanen E., Kaikkonen M.U., Poso A., Varjosalo M, & Levonen A.L. (2024). Oncogenic KEAP1 mutations activate TRAF2-NFκB signaling to prevent apoptosis in lung cancer cells. Redox Biology, 69, 103031.
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4

Artemisinin Modulates Mesangial Cell Responses

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Mouse mesangial cells (SV40 MES13) were purchased from the cell bank of Chinese Academy of Sciences (Shanghai, China). High DMEM (GIBCO, USA) and F12 (GIBCO, USA) were mixed at a ratio of 3:1, and then add 5% Fetal bovine serum (FBS) (BI, USA). The cells were maintained at 37°C in 5% CO2. Artemisinin was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). BSA was purchased from Beijing Solarbio Science & Technology Co., Ltd. (China). Lipopolysaccharides (LPS) were purchased from Sigma-Aldrich (USA); Primary antibodies against AKT, p-AKT and Tubulin were acquired from Abmart Shanghai Co., Ltd. (China); Primary antibodies against Lamin B were purchased from Wanleibio Co., Ltd. (China); Antibodies against Nrf2 were purchased from Proteintech (China)and IgA-FITC was purchased from Abcam (Cambridge, UK). Urine protein kits were obtained from Cloud-Clone Corp (Shanghai, China). Malondialdehyde (MDA) assay kits were obtained from Beyotime Co., Ltd. (China). Levels of blood urea nitrogen (BUN), serum creatinine (SCr) were determined using assay kits, Nanjing Jiancheng Bioengineering Institute (China).
Yang W.G., Sun A., Zhu R., Liu N., He W.J, & Liu L.L. (2023). Exploration of Artemisinin Against IgA Nephropathy via AKT/Nrf2 Pathway by Bioinformatics and Experimental Validation. Drug Design, Development and Therapy, 17, 1679-1697.
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