Hy 10231

For HIF‐1α inhibition, mice were administered PX‐478 (5 mg/kg, HY‐10231, MedChemExpress) in phosphate‐buffered saline (PBS) solution by oral gavage every other day, and the control mice received an equivalent amount of PBS. For NLRP3 inhibition, mice were gavaged with MCC950 (20 mg/kg, HY‐12815, MedChemExpress) or vehicle (.9% NaCl) every day for 5 days/week. For Drp1 inhibition, mice were given mitochondrial division inhibitor 1 (Mdivi‐1) (20 mg/kg, dissolved in dimethyl sulfoxide (DMSO), HY‐15886, MedChemExpress) intraperitoneally, and the control mice were injected with the same volume of DMSO. For ROS scavenging, mice were intraperitoneally injected with mito‐TEMPO (MT, dissolved in PBS, 10 mg/kg, HY‐112879, MedChemExpress) every other day, and the mice in the control group were given an equal volume of PBS intraperitoneally.

We obtained 293 T cells from our lab, and we purchased A549 and H460 human NSCLC cell lines from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS, Gibco), penicillin (100 U/mL), and streptomycin (100 U/mL) at 37°C in 5% CO2.
We cocultured A549 and H460 cells with Cycloheximide (400 μM, HY-12320, MedChemExpress, USA) for 2 hours or PX-478 (10 μM, HY-10231, MedChemExpress, USA) for 24 hours, or α-KG (1 mM, 75890, Sigma, USA) for 24 hours before subsequent examinations.