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5 protocols using tributyrin

1

Purification and Characterization of Lipases

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Ampicillin was obtained from Wako Pure Chemical Industries Ltd (Osaka, Japan). Anhydrotetracycline and Strep-Tactin-Sepharose were purchased from IBA. Triton X-100, ethylenediaminetetraacetic acid (EDTA), urea, l-arginine hydrochloride, l-cystine dihydrochloride, l-cysteine, bovine serum albumin (BSA), Tween 20 and 5, 5′-dithiobis-2-nitrobenzoic acid (DTNB) were obtained from Wako Pure Chemical Industries Ltd. Desthiobiotin and Orlistat were purchased from Sigma-Aldrich (Saint Louis, MO). Phenylmethylsulfonyl fluoride (PMSF) was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Rabbit anti-HPL polyclonal IgGs and anti-Strep-tag II (ST II) monoclonal IgG were obtained from Abcam (Cambridge, UK) and IBA GmbH (Gottingen, Germany), respectively. Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG and anti-mouse IgG antibodies were from Kirkegaard and Perry Laboratories (Washington, DC, USA). Tributyrin was purchased from Wako Pure Chemical Industries Ltd (Osaka, Japan). Porcine colipase was from AbD Serotec (Kidlington, UK). Gel-filtraion FPLC columns, HiPrep 16/60 Sephacryl S-200 HR were purchased from GE Healthcare.
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2

Silver Paste Composition Preparation

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Example 4

As the silver particles, AgC239 (FUKUDA METAL FOIL & POWDER Co., Ltd.) was used. As the solvent, tributyrin (boiling point 305° C., Wako Pure Chemical Industries, Ltd.), butyl stearate (boiling point 343° C., Wako Pure Chemical Industries, Ltd.), and stearic acid (boiling point 376° C., Wako Pure Chemical Industries, Ltd.) were used. The silver particles and the solvent were kneaded for 15 minutes with a stone mill at the blending ratio shown in Table 1 to manufacture a silver paste composition. The properties of this silver paste composition were measured to obtain the result shown in Table 1.

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3

Silver Paste Composition Formulation

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Example 5

As the silver particles, K-0082P (METALOR) was used. As the solvent, tributyrin (boiling point 305° C., Wako Pure Chemical Industries, Ltd.), butyl stearate (boiling point 343° C., Wako Pure Chemical Industries, Ltd.), and stearic acid (boiling point 376° C., Wako Pure Chemical Industries, Ltd.) were used. The silver particles and the solvent were kneaded for 15 minutes with a stone mill at the blending ratio shown in Table 1 to manufacture a silver paste composition. The properties of this silver paste composition were measured to obtain the result shown in Table 1.

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4

Silver Paste Composition Preparation

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Example 3

As the silver particles, AgC239 (FUKUDA METAL FOIL & POWDER Co., Ltd.) and K-0082P (METALOR) were used. As the solvent, tributyrin (boiling point 305° C., Wako Pure Chemical Industries, Ltd.), butyl stearate (boiling point 343° C., Wako Pure Chemical Industries, Ltd.), and stearic acid (boiling point 376° C., Wako Pure Chemical Industries, Ltd.) were used. The silver particles and the solvent were kneaded for 15 minutes with a stone mill at the blending ratio shown in Table 1 to manufacture a silver paste composition. The properties of this silver paste composition were measured to obtain the result shown in Table 1.

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5

Silver Paste Composition Preparation

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Example 2

As the silver particles, AgC239 (FUKUDA METAL FOIL & POWDER Co., Ltd.) and K-0082P (METALOR) were used. As the solvent, tributyrin (boiling point 305° C., Wako Pure Chemical Industries, Ltd.) and stearic acid (boiling point 376° C., Wako Pure Chemical Industries, Ltd.) were used. The silver particles and the solvent were kneaded for 15 minutes with a stone mill at the blending ratio shown in Table 1 to manufacture a silver paste composition. The properties of this silver paste composition were measured to obtain the result shown in Table 1.

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