Ethidium bromide treatment, to induce mtDNA depletion, was performed according to Rahn and colleagues6 (link). Exposure to CLO (Clofilium tosylate, C2365, Sigma–Aldrich) was performed by incubating zebrafish larvae for 7 days (from 5 dpf to 12 dpf) in fish water-diluted drug, without methylene blue.
Clofilium tosylate
Manufactured by Merck Group
Sourced in Japan
Clofilium tosylate is a laboratory reagent used in research settings. It is a chemical compound with the molecular formula C₁₅H₁₆ClNO₃S. This product is designed for use in scientific experimentation and analysis, but its specific applications and intended uses are not provided in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Benzalkonium chloride, by Merck Group (1 mentions)
Filipin complex, by Merck Group (1 mentions)
Paxilline, by Merck Group (1 mentions)
Quinidine, by Merck Group (1 mentions)
Tea chloride, by Merck Group (1 mentions)
Dq red bsa, by Thermo Fisher Scientific (1 mentions)
Ns1619, by Merck Group (1 mentions)
Fura 2 am, by Thermo Fisher Scientific (1 mentions)
Dibac4 3, by Dojindo Laboratories (1 mentions)
2 apb, by Bio-Techne (1 mentions)
4 protocols using clofilium tosylate
1
Ethidium Bromide-Induced mtDNA Depletion
2
Bacterial Culture Conditions and Genetic Manipulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacteria were cultured in lysogeny broth (LB) broth at 37°C unless otherwise stated. Clostridium difficile was grown with brain heart infusion medium with yeast extract (BHIS) and reinforced clostridial medium (RCM). Carbenicillin (75 µg/ml), kanamycin (25 µg/ml), gentamicin (10 µg/ml), chloramphenicol (10 µg/ml), and tetracycline (10 µg/ml) were used for selection. Benzalkonium chloride was from Sigma and was a mixture of the C12 and C14 alkyl chain lengths. Clofilium tosylate and otilonium bromide (OB) were purchased from Sigma. Gene deletion and complementation were conducted as previously described (23 (link)).
3
Membrane Protein Purification and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents were dissolved and stored in DMSO or water. DQ-BSA-red was from Life Technologies; anti-SLO-1 antibodies were purchased from NeuroMab; vacuolin-1 was from Calbiochem; ML-SA1 was from Princeton BioMolecular Research Inc.; quinidine, paxilline, clofilium tosylate, NS1619, IBTX, TEA chloride, and filipin complex were purchased from Sigma-Aldrich.
4
Pharmacological Agents Procurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sources of pharmacological agents were as follows: clofilium tosylate (Sigma-Aldrich, Tokyo, Japan), DiBAC4(3), (Dojindo, Kumamoto, Japan), Fura 2-AM (Invitrogen), and 2-APB (Tocris Bioscience, Ellisville, MO, USA). All others were obtained from Sigma-Aldrich or Wako Pure Chemical Industries.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!