M3z microscope

Three days after cell implantation (EDD11), tumors were treated topically with pPEDF (1.25 μg/ml, corresponding to PEDF concentration in 5% ascites), ascites (5%), “ascites-PEDF” (5% equivalent ascites) or FBS (5%) with 100 μM Ptx. CAMs with untreated implanted cells served as controls. On EDD13 tumor size was monitored using a Wild Heerbrugg M3Z microscope at 10× magnification with a Lumenera INFINITY2-1 CDD camera equipped with Infinity Capture Software.

Three days after the grafting (EDD11), available tumors were treated topically with 100 μM equivalent Ptx. Eggs were returned to the incubator for 2 days (EDD13). Tumor size was monitored using a Wild Heerbrugg M3Z microscope at 10x magnification with a Lumenera INFINITY2-1 CDD camera with Infinity Capture Software.

Fertilized eggs (animal facility of the University of Geneva, Geneva, Switzerland) were incubated at 38° C at 80% relative humidity with periodic rotation. Rotation was stopped on egg development day (EDD) 4 and a hole was drilled at the narrow apex, which was sealed with adhesive tape On EDD8, the hole in the eggshell was enlarged to allow the access to the CAM. After gentle scratching of the CAM with a needle tip, 2 × 10⁶ COV318, SKOV3, CTRL-SKOV3 or PEDF-SKOV3 cells suspended in 30 ul Geltrex^® (Life technologies, Basel, Switzerland) were implanted on the CAM and the hole was sealed with Parafilm^®. On EDD11 tumor growth was monitored using a Wild Heerbrugg M3Z microscope at 10× magnification with a Lumenera INFINITY2-1 CDD camera equipped with Infinity Capture Software.