Adult male Long-Evans (Envigo, Indianapolis, IN; n=32), Sprague Dawley (Envigo, Frederick, MD; n=16), and Wistar (Charles River, Raleigh, NC; n=32) rats were P60 upon arrival and allowed to acclimate to the vivarium for at least one week before experiments began. All rats were singly housed in standard plexiglass cages in a temperature-controlled room on a reverse light/dark cycle (lights on at 22:00). Teklad 7912 standard chow (Envigo) and water were provided ad libitum unless otherwise indicated. Figure 1 depicts the experimental procedures described below. All experiments were approved by the University of Illinois at Chicago Institutional Animal Care and Use Committee and adhered to the NIH Guidelines for the Care and Use of Laboratory Animals.
Sprague dawley
Manufactured by Inotiv
Sourced in United States
Sprague-Dawley is a widely used outbred albino rat strain. It is commonly used in laboratory research, particularly in the fields of toxicology and pharmacology.
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8 protocols using sprague dawley
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Acclimation and Housing of Rodent Models
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Rodent Behavioral Neuroscience Protocols
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Sprague Dawley Rat Housing and Handling
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Adult male and female Sprague Dawley (9–10 weeks, 20 males weighing ~300 and 24 females ~200 g) were obtained from Envigo (Indianapolis, IN, USA). Upon arrival rats were individually housed in a temperature-controlled vivarium (22 °C) with ad libitum access to water and standard rat chow (LabDiet 5053, LabDiet, St. Louis, MO, USA) and acclimated to a 12 h:12 h reverse light/dark cycle for one week. Male and female rats were housed in separate rooms and scrubs, coats, and gloves were changed between handling males and females. Rats were handled and weighed daily beginning during the acclimation period and throughout the experiment. All procedures were approved by the Institute of Animal Care and Use Committee at the University of Georgia (Athens, GA, USA), the protocol numbers were A2020 06-022-Y2-A4 and A2020 06-012-Y2-A16.
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Sprague-Dawley Rat Acclimation Protocol
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The Armed Forces Radiobiology Research Institute (AFRRI) Institutional Animal Care and Use Committee (IACUC) approved all animal use prior to initiation under protocol number 2016-05-006. All procedures were conducted in compliance with the Guide for the Care and Use of Laboratory Animals [22 ] in an Association for Assessment and Accreditation of Laboratory Animal Care- (AAALAC-) accredited facility. Two hundred and eighty-eight male Sprague-Dawley (Rattus norvegicus) rats, approximately 30 days old and weighing 75-100 g, were obtained from Envigo (Barrier 208A, Frederick, MD, USA). After arriving at the vivarium, animals were allowed to acclimate for at least two weeks. Rats were pair-housed throughout the study in plastic microisolator cages with filter tops. Teklab Sani-Chips (Envigo) were used as bedding and changed 2-3 times per week. Vivarium rooms were maintained at 21 ± 2°C with 30-70% humidity. A 12 : 12 h light : dark cycle was maintained with lights on at 0600. Rats were fed a standard rodent chow (Teklad Global Rodent Diet 8604, Envigo) with water available ad libitum.
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Sprague-Dawley Rat Experiment Protocols
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Male rats (Sprague-Dawley (200–230 g, Envigo, Milan, Italy)) were used throughout. The University of Messina Review Board for animal care (OPBA) approved the study. All animal experiments agree with the new Italian regulations (D. Lgs 2014/26), EU regulations (EU Directive 2010/63), and the ARRIVE guidelines.
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Sprague-Dawley Rat Model Study
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Male rats (Sprague-Dawley (200–230 g, Envigo, Milan, Italy)) were used throughout this study. The University of Messina Review Board for animal care (OPBA) approved the study. All the animal experiments were in agreement with the new Italian regulations (D. Lgs 2014/26) and the EU regulations (EU Directive 2010/63).
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Sprague-Dawley Rat Care and Experiments
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All animal care and experimental procedures were carried out in accordance with European Council directives (609/86 and 63/2010) and in compliance with the animal policies issued by the Italian Ministry of Health and the Ethical Committee for Animal Experiments (CESA, University of Cagliari). We made all efforts to minimise pain and suffering and to reduce the number of animals used. Animal studies are reported in compliance with the ARRIVE guidelines (Percie du Sert et al., 2020 (link)) and with the recommendations made by the British Journal of Pharmacology (Lilley et al., 2020 (link)). The total number of animals as well as their suffering was minimised, according to the 3Rs principles. Adult male Sprague–Dawley (SD, 275–300 g) rats (Envigo, Italy) were housed in groups of six at temperature of 22°C ± 2°C and 60% humidity, under a 12 h light/dark cycle (lights on at 7.00 am ) and with ad libitum access to water and food (Mucedola, Italy).
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Rodent Housing and Maintenance for Metabolic Study
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Animals. Adult, male (N=12, 275-300 g) Sprague-Dawley (Rattus norvegicus domesticus) rats were obtained from Envigo (Indianapolis, IN). Animals were housed one per cage (cage size: 21.0 × 41.9 × 20.3 cm) and maintained on a 12:12 h light/dark schedule. To maximize the thermogenic response, animals were maintained at an ambient temperature of 25°C to 28°C and fed a minimum 10% fat diet [20, 21] . Animals received food in the form of a ground powder in glass container in order to habituate them for fecal microbial transplant methods. Animal maintenance and research were conducted in accordance with the eighth edition of the Guide for the Care and Use of Laboratory Animals; as adopted and promulgated by the National Institutes of Health, with protocols approved by the Bowling Green State University Animal Care and Use Committee.
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