Recombinant murine granulocyte macrophage colony stimulating factor rmgm csf

Male C57BL/6 mice (4-8-wk-old) were purchased from the Korean Institute of Chemistry Technology (Daejeon, Korea). C57BL/6 OT-I T cell receptor (TCR) transgenic mice, C57BL/6 OT-II TCR transgenic mice, C57BL/6J TLR-2^-/- (B6.129- Tlr2tm1Kir/J) mice, and C57BL/10 TLR-4^-/- (C57BL/10ScNJ) mice were purchased at 6-8 wk of age from Jackson Laboratory (Bar Harbor, ME). All mice were housed in a specific pathogen-free environment in accordance with the institutional guidelines for animal care. Recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and rmIL-4 were purchased from R&D Systems (Minneapolis, MN). Cytokine enzyme-linked immunosorbent assay (ELISA) kits for murine IL-12p70, tumor necrosis factor-alpha (TNF-α), IL-10, IL-1β, and IL-6 were purchased from R&D Systems. Fluorescein isothiocyanate (FITC)- or phycoerythrin (PE)-conjugated monoclonal antibodies (mAbs) were used to detect the expression of CD11c (HL3), CD80 (16-10A1), CD86 (GL1), I-A^b β-chain (AF-120.1), and H-2K^b (AF6-88.5) by flow cytometry. Isotype-matched control mAbs and the biotinylated anti-CD11c (N418) mAb were purchased from R&D Systems. Anti-phospho-ERK, anti-phospho-p38, anti-phospho-JNK, and anti-tubulin Abs were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). SB203580, SP600135, and U0126 were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Recombinant murine granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and rmIL-4 were purchased from R&D Systems (Minneapolis, MN). Cytokine enzyme-linked immunosorbent assay (ELISA) kits for murine IL-12p70, TNF-α, IL-10, IL-1β, and IL-6 were purchased from R&D Systems. Fluorescein isothiocyanate (FITC)- or phycoerythrin (PE)-conjugated monoclonal antibodies (mAbs) used to detect the expression of CD11c (HL3), CD80 (16-10A1), CD86 (GL1), IAb β-chain (AF-120.1), and H2Kb (AF6-88.5) by flow cytometry, as well as isotype-matched control mAbs and the biotinylated anti-CD11c (N418) mAb, were purchased from R&D Systems. Antibodies against phospho-ERK, ERK, phospho-p38, phospho-JNK, and tubulin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).