PMN-MDSC isolated from decidual tissues of normal pregnancy were cultured at 37°C in humidified air with 5% CO2 in RPMI 1640 (HyClone, USA) supplemented with 10% heat inactivated fetal bovine serum (FBS) (Gibco, USA), 1% penicillin/streptomycin (Gibco, USA) and 5 ng/mL recombinant GM-CSF (R&D Systems, USA). Decidual PMN-MDSC were treated with FasL (10, 100 ng/mL, BioLegend, USA), TRAIL (10, 100 ng/mL, Gibco, USA) or DR5 agonist Bioymifi (10, 50 μM, Selleck, USA) for 24 h and then were collected. In some experiments, PMN-MDSC were preincubated with anti-human DcR2 Ab (10 μg/mL, R&D Systems, USA) for 1 h before exposed to TRAIL. Apoptosis of PMN-MDSC was tested using activated Caspase-3-PE (BD Bioscience, USA) staining or Annexin V Apoptosis Detection Kit (BD Bioscience, USA) according to the manufacturer's instructions.
Activated caspase 3 pe
Manufactured by BD
Sourced in United States
Activated Caspase-3-PE is a fluorescent-labeled antibody that binds to the activated form of the caspase-3 enzyme. Caspase-3 is a key mediator of apoptosis, or programmed cell death. The PE (phycoerythrin) fluorescent label allows for the detection and quantification of activated caspase-3 in cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Bioymifi, by Selleck Chemicals (1 mentions)
Recombinant gm csf, by R&D Systems (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Annexin 5 apoptosis detection kit, by BD (1 mentions)
Streptavidin bv421, by BioLegend (1 mentions)
Cd45r pe, by BioLegend (1 mentions)
Apc cy7, by BioLegend (1 mentions)
Ccr7 pe, by BioLegend (1 mentions)
Gfp fitc, by Rockland Immunochemicals (1 mentions)
Cd4 bv421, by BioLegend (1 mentions)
Icos apc, by BioLegend (1 mentions)
Cxcr4 pe, by BioLegend (1 mentions)
Cxcr5 pe, by BD (1 mentions)
Cd62l apc cy7, by BioLegend (1 mentions)
2 protocols using activated caspase 3 pe
1
Induction of Decidual PMN-MDSC Apoptosis
2
Multicolor Flow Cytometry Antibody Panel
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