Log In Sign up
The largest database of trusted experimental protocols

Nqo 1

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

NQO-1 is a lab equipment product designed for the detection and measurement of the enzyme NAD(P)H:quinone oxidoreductase 1 (NQO1). NQO1 is an enzyme involved in cellular redox processes and plays a role in detoxification. The NQO-1 product provides a tool for researchers to study the activity and expression of this enzyme in various biological samples and experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Hmox1, by Thermo Fisher Scientific (1 mentions) Taqman gene expression assay, by Thermo Fisher Scientific (1 mentions) Omniscript rt kit, by Qiagen (1 mentions) Rneasy fibrous tissue kit, by Qiagen (1 mentions) Prism 7500 sequence detection system, by Thermo Fisher Scientific (1 mentions) β actin, by Thermo Fisher Scientific (1 mentions) Mmp 9, by Thermo Fisher Scientific (1 mentions) Keap1, by Thermo Fisher Scientific (1 mentions) Vcam 1, by Thermo Fisher Scientific (1 mentions) Icam 1, by Thermo Fisher Scientific (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions)

5 protocols using nqo 1

1

Quantifying NQO-1, Nrf2 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Real-time PCR were performed as previously described [9 (link)] using primers for NQO-1, Nrf2, and actin (Life Technologies, Grand Island, NY).
Kong L., Wang Y., Luo M., Tan Y., Cui W, & Miao L. (2017). Prevention of Streptozotocin-Induced Diabetic Nephropathy by MG132: Possible Roles of Nrf2 and IκB. Oxidative Medicine and Cellular Longevity, 2017, 3671751.
+ Open protocol
+ Expand
2

Quantifying Transcriptional Regulators in Skin

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primers and probes (TaqMan® Gene Expression Assays) used to measure the mRNA levels for Keap1, Nrf1, Nrf3, NQO1, GSTP, GCLC, HMOX-1, IL-6, IL-1β, and COX2 were from Life Technologies. Total RNA was extracted from mouse skin using RNeasy Fibrous Tissue Kit (Qiagen Ltd.). Omniscript RT Kit (Qiagen Ltd.) was then used to reverse-transcribe 500 ng of total RNA into cDNA. Real-time PCR was carried out on Perkin Elmer/Applied Biosystems Prism Model 7700 Sequence Detector instrument. The TaqMan data for the mRNA species were normalized using β-actin (mouse ACTB, 4352933E) as an internal control.
Knatko E.V., Ibbotson S.H., Zhang Y., Higgins M., Fahey J.W., Talalay P., Dawe R.S., Ferguson J., Huang J.T., Clarke R., Zheng S., Saito A., Kalra S., Benedict A.L., Honda T., Proby C.M, & Dinkova-Kostova A.T. (2015). Nrf2 activation protects against solar-simulated ultraviolet radiation in mice and humans. Cancer prevention research (Philadelphia, Pa.), 8(6), 475-486.
+ Open protocol
+ Expand
3

qPCR for Ho1 and Nqo1 Gene Expression

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
qPCR was performed as described in our previous studies [39 (link), 40 (link)]. Primers for Ho1 and Nqo1 were purchased from Life Technologies (Grand Island, NY, USA).
Wang Y., Wu H., Xin Y., Bai Y., Kong L., Tan Y., Liu F, & Cai L. (2017). Sulforaphane Prevents Angiotensin II-Induced Testicular Cell Death via Activation of NRF2. Oxidative Medicine and Cellular Longevity, 2017, 5374897.
+ Open protocol
+ Expand
4

Comprehensive Gene Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from tissues and cells using the Trizol method. The cDNA was synthesized using a high-capacity cDNA reverse transcription kit. qRT-PCR was performed on the ABI PRISM 7,500 Sequence Detection System. Primers for NNMT, TNF-α, IL-6, IL-1, TGF-β, Nrf2, heme oxygenase-1 (HO-1), glutamate-cysteine ligase (GCLC), and NADPH quinineoxidoreductase-1 (NQO-1), type 1 collagen, connective tissue growth factor (CTGF), matrix metallopeptidase 9 (MMP-9), α-myosin heavy chain (α-MyHC), brain natriuretic peptide (BNP), and β-actin were purchased from Life Technologies. The mouse housekeeping gene β-actin was used as an internal control. Relative mRNA expression levels were normalized to β-actin. The primer sequences are provided in Supplementary Table 1.
Song Z., Zhong X., Li M., Gao P., Ning Z., Sun Z, & Song X. (2021). 1-MNA Ameliorates High Fat Diet-Induced Heart Injury by Upregulating Nrf2 Expression and Inhibiting NF-κB in vivo and in vitro. Frontiers in Cardiovascular Medicine, 8, 721814.
+ Open protocol
+ Expand
5

Quantitative Real-Time PCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Quantitative real-time PCR was performed as previously described (Wang et al. 2009 (link), Wu et al. 2014) (link). Primers for collagen 4 (Col4), Fn, Gapdh, Nqo1, intercellular adhesion molecule 1 (Icam-1), Ho1, iNos, Keap1, Nrf2 and Vcam-1 were all purchased from Life Technologies.
Zhang H., Liu X., Zhou S., Jia Y., Li Y., Song Y., Wang J, & Wu H. (2018). SP600125 suppresses Keap1 expression and results in NRF2-mediated prevention of diabetic nephropathy. Journal of molecular endocrinology, 60(2).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!