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Dylight 405 donkey anti rabbit

Manufactured by Jackson ImmunoResearch

DyLight™ 405 donkey anti-rabbit is a secondary antibody conjugate produced in donkey. It is designed to detect and bind to rabbit primary antibodies. The DyLight™ 405 fluorophore is used for labeling.

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3 protocols using dylight 405 donkey anti rabbit

1

Senescence Evaluation in Isolated Lung Cells

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Isolated human lung cells were stained for SA-β-Gal using the Cell Event™ Senescence Green Flow Cytometry Assay Kit (Invitrogen™, Thermo Fisher Scientific, C10840), following manufacturer’s instructions. Briefly, after dissociation, lung cells were fixed in 2% PFA (Thermo Scientific, 28908) for 10 min at room temperature. Cells were washed, centrifuged, and resuspended in freshly prepared working solution and incubated for 1–2 h at 37 °C in a dry incubator, protected from the light. After incubation, cell suspensions were washed twice with 1% BSA in D-PBS and permeabilized with 0.1% Tween20® (Sigma, St. Quentin Fallavier, France, P1379) in D-PBS for 15 min on ice. After washing, cells were incubated with anti-LGR6 antibody (1:100; Abcam, Waltham, MA, USA, ab126747) and with APC-conjugated anti-human CD45 and CD31 antibodies (1:1000 and 1:1500; BioLegend, San Diego, CA, USA, 304012 and 303116) for 60 min on ice. Secondary DyLight™ 405 donkey anti-rabbit (1:500; Jackson ImmunoResearch Europe Ltd., 711-475-152) was used to detect LGR6 expression. Samples were run on BD Scientific Canto II Flow Cytometer (BD Bioscience, San Diego, CA, USA, RRID: SCR_018056) and flow cytometry data were analysed with FCS Express™ (version 7, DeNovo Software, Glendale, CA, USA, RRID: SCR_016431).
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2

Multicolor Immunofluorescence Labeling

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Primary antisera used were rabbit anti-Vasa (R. Lehmann laboratory), mouse monoclonal anti-Hts (1B1, DSHB)39 , mouse monoclonal anti-Orb (4H8, DSHB)40 , mouse monoclonal anti-HA (abcam ab130275), chicken anti-GFP (Aves Labs, GFP-1020), mouse monoclonal anti-ATP5A [15H4C4] (abcam ab14748), mouse anti-PDH E1 alpha (Abcam, ab110334) and rabbit anti-phoshpo-PDH E1alpha (S293) (Millipore, AP1062). Secondary antibodies were DyLight™ 405 donkey anti-rabbit, DyLight™ 405 donkey anti-mouse, Cy3 donkey anti-mouse, all from Jackson ImmunoResearch, and Alexa fluor 488 goat anti-chicken from ThermoFisher Scientific.
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3

Multicolor Immunofluorescence Labeling

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Primary antisera used were rabbit anti-Vasa (R. Lehmann laboratory), mouse monoclonal anti-Hts (1B1, DSHB)39 , mouse monoclonal anti-Orb (4H8, DSHB)40 , mouse monoclonal anti-HA (abcam ab130275), chicken anti-GFP (Aves Labs, GFP-1020), mouse monoclonal anti-ATP5A [15H4C4] (abcam ab14748), mouse anti-PDH E1 alpha (Abcam, ab110334) and rabbit anti-phoshpo-PDH E1alpha (S293) (Millipore, AP1062). Secondary antibodies were DyLight™ 405 donkey anti-rabbit, DyLight™ 405 donkey anti-mouse, Cy3 donkey anti-mouse, all from Jackson ImmunoResearch, and Alexa fluor 488 goat anti-chicken from ThermoFisher Scientific.
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