Meso Scale Discovery (MSD) multiplex assay was used to determine CSF levels of interleukin-2 (IL-2), IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-1β, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) (Cat. No. K15049G, MSD, Rockville, MD).
Msd multiplex assay
Manufactured by Mesoscale
Sourced in United States
The MSD multiplex assay is a laboratory equipment product developed by Mesoscale. It is designed to perform multiplexed immunoassays, allowing simultaneous detection and quantification of multiple analytes in a single sample. The core function of the MSD multiplex assay is to provide a platform for sensitive and accurate measurement of various biomolecules, such as proteins, cytokines, or other targets of interest.
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Lab products found in correlation
3 protocols using msd multiplex assay
1
Multiplex Cytokine Profiling in CSF
2
Quantifying Cytokines and Viral Transmission
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Total cellular RNA from cells or homogenized tissues was isolated by RNAzol B (TEL‐TEST, INC) using the manufacturers protocol, and GAPDH was used as a housekeeping gene. The level of pro‐inflammatory cytokines, IL‐1β, IL‐6, IL‐8, and TNF‐α messenger RNA (mRNA), was measured in the NG or control media exposed tissues/cells using RT‐PCR with gene‐specific primers/probes by real‐time RT‐PCR using primers and probes as described as described previously.38 Secreted cytokines in culture supernatant were measured by MSD multiplex assay according to the manufacturers protocol (MESO SCALE DISCOVERY, Rockville, MD 20850 USA). To measure the transmission of virus across tissues, HIV‐Gag38 mRNAs were measured in the HIV‐1‐exposed tissues by RT‐PCR and HIV p24 was measured in the supernatants using a commercially available HIV‐1 p24 ELISA kit according to manufacturers protocol (SAIC‐Fredrick, MD).
3
Multiplex Cytokine Profiling in CSF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Meso Scale Discovery (MSD) multiplex assay was used to determine CSF levels of interleukin-2 (IL-2), IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-1β, tumor necrosis factor α (TNF-α), and interferon γ (IFN-γ) (Cat. No. K15049G, MSD, Rockville, MD).
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