Axiovert 40cfl

Manufactured by Olympus

Sourced in Germany

The Axiovert 40CFL is an inverted microscope designed for routine laboratory work. It features a compact and sturdy construction, providing a stable platform for various microscopy techniques. The microscope is equipped with a CFL (Compact Fluorescent Lamp) illumination system, offering bright and even illumination for both transmitted light and fluorescence applications.

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Lab products found in correlation

Versamax, by Molecular Devices (1 mentions) Oil red o, by Merck Group (1 mentions) Oil red o, by Molecular Devices (1 mentions) Enzyme free cell dissociation buffer, by Thermo Fisher Scientific (1 mentions) Easysep magnet, by STEMCELL (1 mentions) Macs separator, by Miltenyi Biotec (1 mentions) Pe positive selection kit, by STEMCELL (1 mentions) Anti pe microbeads, by Miltenyi Biotec (1 mentions)

3 protocols using axiovert 40cfl

Visualizing Adipocyte Lipid Accumulation

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Lipid accumulation of differentiated adipocytes was visualized and determined by Oil Red O staining kit (ECM950, Millipore). Briefly, differentiated adipocytes were washed with PBS and fixed in 3.7% formaldehyde for 15 minutes, followed by staining with Oil Red O solution (3 g/L) for 15 minutes. After staining, cells were washed twice with water and photographed using a microscope (Axiovert 40CFL, Olympus).

Hu J., Zhou A., Cheung P.C., Zheng B., Zeng S, & Lin S. (2018). Expression of GPR43 in Brown Adipogenesis Is Enhanced by Rosiglitazone and Controlled by PPARγ/RXR Heterodimerization. PPAR Research, 2018, 1051074.

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Quantifying Adipocyte Lipid Content

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Fully differentiated 3T3-L1 cells were washed twice with PBS and fixed in 4% paraformaldehyde for 1 h. Cells were stained with 3 g/L of Oil Red O (Sigma Chemical, St. Louis, MO) in 60% isopropanol at room temperature for 10 min and washed extensively with distilled water. Pictures were taken using a microscope (Axiovert 40CFL; Olympus, Germany). In addition, stained Oil Red O dye was extracted with isopropanol and collected, and the absorbance (O.D. 500 nm) was measured by microplate reader (Versamax; Molecular Devices Corporation, CA, USA).

Park J.Y., Kim Y., Im J.A., You S, & Lee H. (2014). Inhibition of Adipogenesis by Oligonol through Akt-mTOR Inhibition in 3T3-L1 Adipocytes. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 895272.

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Antigen-Presenting Cell Cytokine Assay

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APCs expressing desired HLA, encoded peptides, and surface-bound anti-cytokine antibodies were seeded in 384-well, 96-well, or 10-cm plates, and cultured for 2–8 days. T cells were then added at a ratio between 2:1 and 16:1 along with 25–80 ng/mL PMA. After incubation at 37°C for 16–24 hours (or for time intervals indicated in text), cells were washed with phosphate buffered saline (PBS). Cells were dissociated with 0.25% trypsin-EDTA or enzyme-free cell dissociation buffer (Thermo Fisher Scientific), pooled, and stained with fluorescently-labeled anti-IL2 antibody; alternatively, cells were stained in the plate prior to dissociation. Stained cells were washed with PBS. A subset was imaged (Zeiss Axiovert 40 CFL, Olympus CK40, or Olympus IX73) to assess fluorescence, or analyzed by flow cytometry (BD LSRFortessa). For pulldowns, PE⁺ cells were labeled using Anti-PE MicroBeads (Miltenyi) or PE Positive Selection Kit (StemCell Technologies), and separated from unlabeled cells using a MACS Separator (Miltenyi) or EasySep Magnet (StemCell Technologies).

Lee M.N, & Meyerson M. (2021). Antigen identification for HLA class I- and II-restricted T cell receptors using cytokine-capturing antigen-presenting cells. Science immunology, 6(55), eabf4001.

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