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125iodine

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125Iodine is a radioactive isotope of iodine used in various laboratory applications. It has a half-life of approximately 59.4 days and emits gamma radiation. 125Iodine is commonly used in research and diagnostic procedures where its radioactive properties can be leveraged for analysis and detection purposes.

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Lab products found in correlation

Ova grade 7, by Merck Group (2 mentions) Nap 25 column, by GE Healthcare (2 mentions) Packard cobra gamma counter, by Hewlett-Packard (2 mentions) Recombinant human leptin, by R&D Systems (1 mentions) 1470 wizard, by PerkinElmer (1 mentions) Acetonitrile, by Thermo Fisher Scientific (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Cathepsin b, by Merck Group (1 mentions) Cy5 amine, by Lumiprobe (1 mentions) 2 2 azobis 2 2 imidazolin 2 yl propane dihydrochloride va 044, by Fujifilm (1 mentions) 2 2 azobis 2 4 dimethylvaleronitrile v 65, by Fujifilm (1 mentions)

4 protocols using 125iodine

1

Leptin Biodistribution in Mice

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Recombinant human leptin (R&D Systems) was labelled with 125Iodine (Perkin Elmer) using the Iodogen method (Thermo Scientific). Mice were injected intravenously via the lateral tail vein with 12 ng (14.4 kBq) leptin in a total volume of 100 µL made up with phosphate-buffered solution. Animals were then placed in an individual cage with access to food and water until the specified time when the animal was killed by rapid CO2 asphyxiation with n = 4 for each time point, to observe distribution over a time course. Tissues were dissected and weighed with duplicate samples placed in polypropylene tubes and measured for total γ-radioactivity (1470 Wizard, Perkin Elmer). Background radiation was subtracted from all samples. Due to equipment failure, no data were collected for brain or digestive tract at 120 min.
Blood was collected via cardiac puncture and transferred to vial containing 20 IU heparin and measured in duplicate. Total blood volume was calculated as 84.7 mL/kg body mass as previously reported (34 (link)). The skin was removed apart from that around the snout and ‘cuffs’ around the limbs and weighed intact. Four samples were taken from the left forelimb, right hindlimb, interscapular and dorsal cervical regions. Gut tissues were measured with contents.
Hart R.A., Dobos R.C., Agnew L.L., Smart N.A, & McFarlane J.R. (2016). Leptin pharmacokinetics in male mice. Endocrine Connections, 6(1), 20-26.
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2

Synthesis of Polymeric Prodrugs for Cancer Therapy

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Common solvents methanol, acetonitrile, dimethylformamide (DMF), dichloromethane (DCM) were from Fisher Scientific (Pittsburgh, PA) as HPLC grade and used directly. Diisopropylethylamine (DIPEA), trifluoroacetic acid (TFA), papain (EC 3.4.22.2, from papaya latex) and cathepsin B (EC 3.4.22.1, from bovine spleen) were from Sigma-Aldrich (St. Louis, MO). Epirubicin (EPI) was a kind gift from Prof. Kui Luo (Sichuan University, China). HATU was from AAPPTEC (Louisville, KY). 2,2-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (VA-044) and 2,2-azobis(2,4-dimethyl valeronitrile) (V-65) were obtained from Wako Chemicals (Richmond, VA). 125Iodine was from Perkin-Elmer (Waltham, MA). Cy3-/Cy5-NHS ester and Cy5-amine were purchased from Lumiprobe (Hallandale Beach, FL). Various monomers including N-(2-hydroxypropyl)methacrylamide (HPMA) [29 ], N-methacryloylglycylphenylalanylleucylglycine (MA-GFLG-OH) [30 ], 3-(N-methacryloylglycylphenylalanylleucylglycyl) thiazolidine-2-thione (MA-GFLG-TT) [31 ], N-methacryloyltyrosinamide (MA-Tyr-NH2) [32 (link)], 2-(N-methacryloylglycylphenylalanylleucylglycine)- N′-Boc-ethylenediamine (MA-GFLG-NH-Boc) [16 (link)], and RAFT agents, 4-cyanopentanoic acid dithiobenzoate (CPA) [33 ] and peptide2CTA (Nα,Nε-bis(4-cyano-4-(phenylcarbonothioylthio)pentanoylglycylphenylalanylleucylglycyl)lysine) [15 (link)], were synthesized as previously described.
Yang J., Zhang R., Radford D.C, & Kopeček J. (2015). FRET-trackable biodegradable HPMA copolymer-epirubicin conjugates for ovarian carcinoma therapy. Journal of controlled release : official journal of the Controlled Release Society, 218, 36-44.
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3

Labeling and Tracing Ovalbumin in Mice

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OVA grade VII (Sigma, A7641) was labeled with 125Iodine (Perkin-Elmer) under a fume hood. Briefly, 250 μCi Na125I in 135.5 μl 0.1 N NaOH was prepared and immediately mixed with an equal volume of 0.1 N HCl containing 6.67 nmol ICl. The iodine mixture was immediately added to 1 ml of 100 μg OVA in 1M glycine, pH 9.5, 100 mM NaCl (2.26 μM OVA, equivalent to an Iodine: OVA molar ratio of 3:1), and the solution incubated at RT for 10 min. Unincorporated iodine was removed by purifying the sample on two consecutive NAP25 columns (GE Healthcare). 125I-OVA was eluted in H2O and kept at 4 °C in 1× PBS by appropriate addition of 10× PBS. Amount of labeling was determined on a Packard Cobra gamma counter and an estimated to be 95000 CPM/μg OVA. Protein purity and integrity were confirmed on a Coomassie gel and specific labeling of OVA by exposing the gel to autoradiography film. To determine OVA uptake and clearance, mice were fasted for 3 h and then gavaged at 12 pm with 50 mg OVA grade III in 200 μl PBS 300,000 CPM 25I-OVA (about 3 μg OVA). Submandibular blood and urine were collected in 5 μl aliquots; whole organs were taken at indicated time points and weighed. Radioactivity was measured using on a Packard Cobra gamma counter.
Esterházy D., Loschko J., London M., Jove V., Oliveira T.Y, & Mucida D. (2016). Classical dendritic cells are required for dietary antigen-mediated peripheral regulatory T cell and tolerance induction. Nature immunology, 17(5), 545-555.
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4

Labeling and Tracing Ovalbumin in Mice

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OVA grade VII (Sigma, A7641) was labeled with 125Iodine (Perkin-Elmer) under a fume hood. Briefly, 250 μCi Na125I in 135.5 μl 0.1 N NaOH was prepared and immediately mixed with an equal volume of 0.1 N HCl containing 6.67 nmol ICl. The iodine mixture was immediately added to 1 ml of 100 μg OVA in 1M glycine, pH 9.5, 100 mM NaCl (2.26 μM OVA, equivalent to an Iodine: OVA molar ratio of 3:1), and the solution incubated at RT for 10 min. Unincorporated iodine was removed by purifying the sample on two consecutive NAP25 columns (GE Healthcare). 125I-OVA was eluted in H2O and kept at 4 °C in 1× PBS by appropriate addition of 10× PBS. Amount of labeling was determined on a Packard Cobra gamma counter and an estimated to be 95000 CPM/μg OVA. Protein purity and integrity were confirmed on a Coomassie gel and specific labeling of OVA by exposing the gel to autoradiography film. To determine OVA uptake and clearance, mice were fasted for 3 h and then gavaged at 12 pm with 50 mg OVA grade III in 200 μl PBS 300,000 CPM 25I-OVA (about 3 μg OVA). Submandibular blood and urine were collected in 5 μl aliquots; whole organs were taken at indicated time points and weighed. Radioactivity was measured using on a Packard Cobra gamma counter.
Esterházy D., Loschko J., London M., Jove V., Oliveira T.Y, & Mucida D. (2016). Classical dendritic cells are required for dietary antigen-mediated peripheral regulatory T cell and tolerance induction. Nature immunology, 17(5), 545-555.
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