Rabbit antibody against cleaved caspase 3

These methods were similar to those described previously (Sykora et al., 2015). Briefly, sections of olfactory bulb were immunostained with a mouse monoclonal antibody against phosphorylated γH2AX (Ser139) conjugated to Alexa Fluor^® 488 (Cell Signaling Technology, MA) to evaluate DNA double‐strand breaks, and with a rabbit antibody against cleaved caspase 3 (Cell Signaling Technology, MA) to identify apoptotic cells. Immunostained sections were mounted in Vectashield hard set with DAPI and analyzed under an Axiovert 200M Zeiss microscope using 40X and 60X objective lenses. Nuclear foci of γH2AX and cellular cleaved caspase 3 immunoreactivity were quantified in three random areas on at least three sections of OB per mouse, and data are presented as fold of increase compared to WT.

The rabbit antibody against HSPB5 was bought from Abcam (Cambridge, USA). The rabbit antibody against cleaved caspase 3 was from Cell Signaling Technology (Danvers, USA). The mouse antibodies against GFP and β-actin were from Proteintech (Wuhan, China). Phalloidin-iFluor 594 was from Abcam. Alexa Fluor 594 goat anti-rabbit IgG was from Thermo Fisher Scientific (Waltham, USA). Citrate synthase, DAPI and 302 serum-free medium were from Sigma (St. Louis, USA). PNGase F was from NEB (Beverly, USA). Caspase-Glo 3/7 assay kit was from Promega (Madison, USA). pEGFP-Htt^ex1-Q74 plasmid was from Addgene (Cambridge, USA). HiTrap Protein A HP column was from GE Healthcare (Little Chalfont, UK).