Phenformin p7045

Manufactured by Merck Group

Phenformin (P7045) is a laboratory reagent supplied by Merck Group. It is a compound used for research purposes. No further details on its core function or intended use can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Anti ha antibody mms 101r, by Fortrea (1 mentions) Cycloheximide c4859, by Merck Group (1 mentions) Aicar a9978, by Merck Group (1 mentions) Anti lamin a c sc 6215, by Santa Cruz Biotechnology (1 mentions) Rapamycin r 5000, by LC Laboratories (1 mentions) Mg132 m 1157, by AG Scientific (1 mentions) Brefeldin a, by Cayman Chemical (1 mentions) Prt4165, by Abcam (1 mentions) Bay 876, by Merck Group (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Thapsigargin, by Santa Cruz Biotechnology (1 mentions) Isrib, by Apexbio (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Sb203580, by Selleck Chemicals (1 mentions) A 769662, by LC Laboratories (1 mentions) Torin 1 s2827, by Selleck Chemicals (1 mentions) Cycloheximide c7698, by Merck Group (1 mentions) Rapamycin r8781, by Merck Group (1 mentions)

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Phenformin (71 citations) P7045 (2 citations)

4 protocols using phenformin p7045

Analysis of AMPK and autophagy regulation

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The following commercially available antibodies were used: anti-AMPKα1 (ab110036), anti-AMPKα2 (ab3760), anti-ATG14 (ab173943), anti-FOXO3a (ab12162), anti-histone H3 (ab1791), anti-H3R17me2 (ab8284), anti-H3K4me3 (ab8580), anti-H3K9me3 (ab8898), anti-H3K36me3 (ab9050), anti-PI3K class 3 (ab124905), and anti-TFEB (ab2636) antibodies were purchased from Abcam. Anti-AMPK (2532), anti-ATG12 (4180), anti-CARM1 (3379 for immunblotting, 12495 for immunoprecipitation and ChIP), anti-LC3 (2775), anti-phospho-AMPKαT172 (2535), anti-phospho-FOXO3a S413 (8174), anti-SQSTM1/p62 (5114), and anti-TFE3 (14779) antibodies were from Cell Signaling Technology. Anti-SKP2 (sc-7164), anti-CUL1 (sc-17775), anti-tubulin (sc-8035), and anti-Lamin A/C (sc-6215) were from Santa Cruz Biotechnology. Anti-Flag (F3165), anti-ULK1 (A7481) and anti-β-actin (A1978) antibodies were from Sigma, anti-HA antibody (MMS-101R) from Covance, and anti-tubulin antibody (LF-PA0146A) from Abfrontier. The following chemicals were used in this study: rapamycin (R-5000) was purchased from LC laboratories, cycloheximide (C4859), AICAR (A9978) and phenformin (P7045) from Sigma, bafilomycin A1 (11038) and ellagic acid from Cayman (10569), compound C from Calbiochem (171260), and MG132 (M-1157) from A.G. Scientific.

Shin H.J., Kim H., Oh S., Lee J.G., Kee M., Ko H.J., Kweon M.N., Won K.J, & Baek S.H. (2016). AMPK–SKP2–CARM1 signalling cascade in transcriptional regulation of autophagy. Nature, 534(7608), 553-557.

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CRISPR/Cas9-Mediated Knockout of AMPK and ATF4

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Knockout of AMPK α1/α2 and ATF4 in UMRC6 cell line was done by using sgRNAs and CRISPR/Cas9 technology as follows. The sgRNAs were cloned into the lentiviral lentiCRISPR v2 vector. A LentiCRISPR v2 vector containing sgRNAs against ATF4 was described in our previous publication (30 (link)). All constructs were confirmed by DNA sequencing. pGIPZ-shRNAs against BMI1 were obtained from the Functional Genomics Core at The University of Texas MD Anderson Cancer Center. Other reagents were purchased as follows: 2-deoxy-D-glucose (2DG; D6134), metformin (PHR1084), phenformin (P7045), tunicamycin (T7765), and N-acetyl-cysteine (A7250) from Sigma; A-769662 (LC laboratories, A-1803); 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR; ThermoFisher Scientific, A611700); ISRIB (Apexbio, B3699); brefeldin A (Cayman Chemical, #11861); thapsigargin (Santa Cruz, sc-24017A); PRT-4165 (Abcam, ab146003); BAY-876 (Sigma, SML1774); SP600125 (Selleckchem, S1460); and SB203580 (Selleckchem, S1076).

Zhang Y., Shi J., Liu X., Xiao Z., Lei G., Lee H., Koppula P., Cheng W., Mao C., Zhuang L., Ma L., Li W, & Gan B. (2020). H2A monoubiquitination links glucose availability to epigenetic regulation of the endoplasmic reticulum stress response and cancer cell death. Cancer research, 80(11), 2243-2256.

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Bacterial Expression of Truncated CaMKK2.1

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For bacterial CaMMK2.1 expression, the human DNA sequence for truncated CaMKK2.1 (156–588), encompassing the kinase domain, was generated with a NH₂-terminal GST-tag (PreScission cleavable) and COOH-terminal 6xHis-tag and cloned into pGEX-6P-1 using NdeI/BamHI restriction sites by Gene Universal (Newark, Delaware, United States). Other plasmids used in this study have been described previously [14 (link),41 (link)]. pMT2-HA-γ1-R299G mutant construct was generated from pMT2-HA-γ1 [41 (link)] by site-directed mutagenesis using QuikChange site-directed mutagenesis kits (Stratagene) and sequence verified. MK-8722 (AOB33226) and PF-739 (AOB33584) were from AOBIOUS. AMP (A1752), ADP (A2754), ATP (A2383) and phenformin (P7045) were from Sigma–Aldrich.

Ovens A.J., Gee Y.S., Ling N.X., Yu D., Hardee J.P., Chung J.D., Ngoei K.R., Waters N.J., Hoffman N.J., Scott J.W., Loh K., Spengler K., Heller R., Parker M.W., Lynch G.S., Huang F., Galic S., Kemp B.E., Baell J.B., Oakhill J.S, & Langendorf C.G. (2022). Structure-function analysis of the AMPK activator SC4 and identification of a potent pan AMPK activator. Biochemical Journal, 479(11), 1181-1204.

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Validated Pharmacological Modulators

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Rapamycin (R8781), AMP (A1752), ATP (A2383), phenformin (P7045), 2-deoxy-D-glucose (2DG; D6134), H2O2 (18304) and cycloheximide (C7698) were from Sigma-Aldrich. Torin1 (S2827) was from Selleckchem and A769662 (ab120335) was from Abcam.

Smiles W.J., Ovens A.J., Yu D., Ling N.X., Morrison K.R., Hoque A., Scott J.W., Galić S., Langendorf C.G., Kemp B.E., Peterson J., & Oakhill J.S. (2021). Novel torin1-sensitive phosphorylation sites on the metabolic regulator AMPK revealed by label-free mass spectrometry.

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