Colo829

Manufactured by American Type Culture Collection

Sourced in United States

The COLO829 is a cell line derived from a human skin melanoma. It is a well-established model for the study of melanoma biology and drug development. The cell line is maintained and distributed by the American Type Culture Collection (ATCC) for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

27 protocols using colo829

Culturing Metastatic Melanoma and EBV B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen vials of the metastatic melanoma cell-line COLO829 (CRL-1974) and EBV transformed B lymphoblast cells from the same individual (COLO829BL, CRL-1980) were purchased from ATCC via Cedarlane (Burlington, Canada). COLO829BL cells were cultured to passage #3 at 37 °C in RPMI-1640 media supplemented with Fetal Bovine Serum (10%). COLO829 cells were cultured to passage #14 at 37 °C in RPMI-1640 media supplemented with Fetal Bovine Serum (10%).

Craig D.W., Nasser S., Corbett R., Chan S.K., Murray L., Legendre C., Tembe W., Adkins J., Kim N., Wong S., Baker A., Enriquez D., Pond S., Pleasance E., Mungall A.J., Moore R.A., McDaniel T., Ma Y., Jones S.J., Marra M.A., Carpten J.D, & Liang W.S. (2016). A somatic reference standard for cancer genome sequencing. Scientific Reports, 6, 24607.

+ Open protocol

+ Expand

Cytotoxicity of Novel Compounds on Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Compounds 7–14 and doxorubicin were tested for cytotoxic activity in vitro against panel of human cancer cell lines: melanoma (Colo-829, ATCC, Rockville, MD, USA), ovarian cancer (SK-OV-3, ATCC, Rockville, MD, USA), breast cancer (T47D, MCF-7, and MDA-MB-231, ATCC, Rockville, MD, USA) and lung cancer (A549, ATCC, Rockville, MD, USA). The cultured cells were kept at 37 °C and 5% CO₂. The cells were seeded (5 × 10⁴ cells/well/100 mL DMEM supplemented with 10% FCS and streptomycin/penicillin) using 96-well plates (Nunc Thermo Fisher Scientific, Waltham, MA, USA). The tested compounds 7–14 with the concentration of 1–100 µg/mL DMSO were inducted with the cancer cells for 72 h. The WST-1-formazan (proliferation reagent WST-1 assay, Roche Diagnostics, Mannheim, Germany) was detected using a microplate reader at 450 nm. Results were expressed as a mean value of at least three independent experiments performed in triplicate.

Kadela-Tomanek M., Jastrzębska M., Chrobak E., Bębenek E, & Latocha M. (2022). Hybrids of 1,4-Quinone with Quinoline Derivatives: Synthesis, Biological Activity, and Molecular Docking with DT-Diaphorase (NQO1). Molecules, 27(19), 6206.

+ Open protocol

+ Expand

Characterization of FR-resistant cancer cell lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human melanoma cell line COLO829, human breast cancer cell line HCC38, and murine colon tumor cell line CT26 were purchased from ATCC (Manassas, VA, USA). Human CRC cell lines Caco2, DLD1, HCT116, LoVo, HT29, and RKO were gifts from Dr. Hirofumi Yamamoto (Osaka University Graduate School of Medicine, Japan). All human cell lines were authenticated by ATCC using DNA profiling. Cells were maintained in appropriate media as follows: DMEM (DLD1 and HCT116), RPMI (HT29, RKO, COLO829, CT26, and HCC38), and Hanks-F12K (LoVo) supplemented with 10% fetal bovine serum, 10,000 units penicillin, 10 mg/ml streptomycin, and 25 μg/ml amphotericin B. Culture media and fetal bovine serum were obtained from Life Technologies Japan (Tokyo, Japan). All cells were grown at 37°C in a humidified incubator with 5% CO₂. To obtain FR-resistant cells, cells were cultured in the presence of high concentrations of FR (20 ng/ml for DLD1 and 10 ng/ml for all other cell lines).

Yamano T., Kubo S., Yano A., Kominato T., Tanaka S., Ikeda M, & Tomita N. (2019). Splicing modulator FR901464 is a potential agent for colorectal cancer in combination therapy. Oncotarget, 10(3), 352-367.

+ Open protocol

+ Expand

Karyotyping Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cancer cell lines (COLO-829 ATCC CRL-1974, COLO-829BL ATCC CRL-1980, HCC-1143 ATCC CRL-2321, HCC-1143 BL ATCC CRL-2362, HCC-1187 ATCC CRL-2322 and HCC-1187BL ATCC CRL-2323) were obtained from ATCC¹⁸. The cell lines were cultured using the recommendations from ATCC. Cultured cells were split into two aliquots for metaphase chromosome preparation and karyotype analysis. Representative images and karyotypes are reported in Supplemental Fig. S1. The number of passages is indicated in Supplemental Table 1.

Arora K., Shah M., Johnson M., Sanghvi R., Shelton J., Nagulapalli K., Oschwald D.M., Zody M.C., Germer S., Jobanputra V., Carter J, & Robine N. (2019). Deep whole-genome sequencing of 3 cancer cell lines on 2 sequencing platforms. Scientific Reports, 9, 19123.

+ Open protocol

+ Expand

Culturing Melanocyte and Melanoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human skin melanotic melanoma cell line, COLO 829, was acquired from ATCC (CRL-1974™, USA). The cells were cultured in RPMI 1640 medium with the addition of inactivated fetal bovine serum to a final concentration of 10%, penicillin (100 U/mL), neomycin (10 µg/mL) and amphotericin B (0.25 µg/mL). Human epidermal melanocytes, neonatal, lightly (HEMn-LP) and darkly pigmented (HEMn-DP) were purchased from Cascade Biologics, UK. The experiments were performed on melanocytes from passages 6 to 10. The growth medium was supplemented with a human melanocyte growth supplement-2 (HMGS-2) as well as antibiotics: penicillin (100 U/mL), neomycin (10 μg/mL) and amphotericin B (0.25 μg/mL). Cells were grown in a 5% CO₂ incubator CB 160 (BINDER, Tuttlingen, Germany) at 37 °C with 5% relative humidity.

Rok J., Rzepka Z., Beberok A., Pawlik J, & Wrześniok D. (2020). Cellular and Molecular Aspects of Anti-Melanoma Effect of Minocycline—A Study of Cytotoxicity and Apoptosis on Human Melanotic Melanoma Cells. International Journal of Molecular Sciences, 21(18), 6917.

+ Open protocol

+ Expand

Generating BRAF Inhibitor Resistant Melanoma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

General reagents were purchased from Sigma (St. Louis, MO, USA), except PLX4720, which was from 3way Pharm (Shanghai, China). A375, Colo829, SKMEL5 and G361 cells were purchased from the ATCC and maintained in RPMI or DMEM, supplemented with 10% FBS, 1 mM sodium pyruvate, 2 mM glutamine and 1% penicillin/streptomycin. To generate BRAF inhibitor resistant clones cells were cultured in increasing concentrations of PLX4720 (0.1–1 μM) and the resistant clones were maintained in 1 μM PLX4720 thereafter.

Baenke F., Chaneton B., Smith M., Van Den Broek N., Hogan K., Tang H., Viros A., Martin M., Galbraith L., Girotti M.R., Dhomen N., Gottlieb E, & Marais R. (2015). Resistance to BRAF inhibitors induces glutamine dependency in melanoma cells. Molecular Oncology, 10(1), 73-84.

+ Open protocol

+ Expand

Cell Line Treatments for Protein and Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lines were obtained from A. Houghton (Memorial Sloan-Kettering Cancer Center), except for Colo829 and A2058 that were purchased from ATCC. All cell lines were maintained in RPMI 1640 (Invitrogen 21870-092), supplemented with 2 mM glutamine, 50 units/mL penicillin, 50 units/mL streptomycin, and 10% FBS (Omega Scientific), and incubated at 37 °C in 5% CO2. Samples for protein and gene expression analysis were plated at 60–80% confluency and incubated for 20–24h. Then treated with PD325901 (50nM), Interferon alpha (20000U/mL, R&D 11100) or Interferon beta (1000U/mL, R&D 11415). Control samples were collected untreated at time of treatment.

Litvin O., Schwartz S., Wan Z., Schild T., Rocco M., Oh N.L., Chen B.J., Goddard N., Pratilas C, & Pe’er D. (2015). Interferon α/β enhances the cytotoxic response of MEK inhibition in melanoma. Molecular cell, 57(5), 784-796.

+ Open protocol

+ Expand

Culturing Human Melanoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human melanoma lines COLO829 and A375 were purchased from ATCC (LGC-Standards). Cultures were performed in RPMI1640-Glutamax (Invitrogen) supplemented with 1% non- essential amino-acids, 1 mM sodium pyruvate (Sigma), 100 μg/ml gentamycin and 10% fetal calf serum (FCS) (Invitrogen).

Girard P., Ponsard B., Charles J., Chaperot L, & Aspord C. (2020). Potent Bidirectional Cross-Talk Between Plasmacytoid Dendritic Cells and γδT Cells Through BTN3A, Type I/II IFNs and Immune Checkpoints. Frontiers in Immunology, 11, 861.

+ Open protocol

+ Expand

Verification of melanoma and HEK-293T cell lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Melanoma cell lines Mewo, WM-3246, WM-3622, WM-3629, WM-3670 and WM-3918 were purchased from Rockland (Rockland Immunochemicals Inc, Limerick, PA), and maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, l-glutamine, and penicillin/streptomycin. Melanoma cell lines COLO829 and C32 were purchased from ATCC (ATCC, Manassas, VA) and maintained according to the provided Culture Methods. HEK-293T cells were purchased from ATCC, and maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, l-glutamine, and penicillin/streptomycin. Jurkart cells were maintained in RPMI-1640 medium supplemented with 10% FBS, l-glutamine, and penicillin/streptomycin. The identity of cell lines used in this study was verified by short tandem repeat analysis using the PowerPlex 1.2 system (Promega). The cell lines were tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kits (Lonza).

He S., Zimmerman M.W., Layden H.M., Berezovskaya A., Etchin J., Martel M.W., Thurston G., Jing C.B., van Rooijen E., Kaufman C.K., Rodig S.J., Zon L.I., Patton E.E., Mansour M.R, & Look A.T. (2021). Synergistic melanoma cell death mediated by inhibition of both MCL1 and BCL2 in high-risk tumors driven by NF1/PTEN loss. Oncogene, 40(38), 5718-5729.

+ Open protocol

+ Expand

Culturing Human Melanoma Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human melanoma lines COLO829 (ATCC Cat# CRL‐1974, RRID:CVCL_1137) and A375 (ATCC Cat# CRL‐7904, RRID:CVCL_0132) were purchased from ATCC (LGC‐Standards).
Cultures were performed in RPMI1640‐Glutamax (Invitrogen) supplemented with 1% non‐essential amino acids, 1 mm sodium pyruvate (Sigma), 100 µg mL⁻¹ gentamycin and 10% foetal calf serum (FCS) (Invitrogen). All cell lines were obtained from ATCC and tested negative for mycoplasma contamination. Cell lines were authenticated by phenotypic analysis and tumor antigen expression by RT‐PCR and flow cytometry.

Girard P., Sosa Cuevas E., Ponsard B., Mouret S., Gil H., Col E., De Fraipont F., Sturm N., Charles J., Manches O., Chaperot L, & Aspord C. (2021). Dysfunctional BTN3A together with deregulated immune checkpoints and type I/II IFN dictate defective interplay between pDCs and γδ T cells in melanoma patients, which impacts clinical outcomes. Clinical & Translational Immunology, 10(11), e1329.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!