Plan apochromat 40 1.4 objective

Representative images were acquired with a Zeiss LSM700 confocal microscope using a Plan-Apochromat 40×/1.4 objective. Worms were immobilized using 1.5% 1-phenoxy-2-propanol (TCI America, Portland, OR) in M9 buffer and mounted on 5% agar slides. 3D reconstructions were done using Zeiss Zen software. Images of GABA neuron synapses show the dorsal cord at the middle part of animals. A Zeiss Axio Imager 2 microscope equipped with Chroma HQ filters was used to score AMsh migration defects and take images to measure Migration Index. For GABA neuron synapse number quantification, a Zeiss Axio Imager 2 microscope equipped with Chroma HQ filters was used to quantify the total number of SNB-1::GFP puncta at the dorsal cord. Each condition represented 3 experiments of at least 50 Day 1 adult animals each unless otherwise noted.