Nup153

The following primary antibodies were used for immunocytochemistry and Western blot analysis: rabbit polyclonal antibodies against lamin B1, Tpr, importin α7 (Abcam, Cambridge, MA), pHIST3 (serine 10), MAP-2 (Merck Millipore, Darmstadt, Germany), actin, βIII-tubulin (Sigma-Aldrich, Milano, Italy), pERK, pCREB (Cell Signaling, Beverly, MA), lamin A/C (Santa Cruz Biotechnology, Dallas, TX), synaptophysin (SySy, Gottingen, Germany), and drebrin (Enzo Life Sciences, Farmingdale, NY); and mouse monoclonal antibodies against Nup153, lamin B2, CREB (Abcam), nuclear pore complex (mAb414; Covance, Princeton, NJ), Lap2β (BD Biosciences, East Rutherford, NJ), Tau (Tau-1; Merck Millipore), ERK (Cell Signaling), importin β (Sigma-Aldrich), and Alexa 488–conjugated Tuj1 (Covance). For Western blot analysis, horseradish peroxidase (HRP)–conjugated secondary antibodies (Bio-Rad, Hercules, CA) were used for detection. For immunofluorescence analyses, Alexa fluorophore–conjugated anti-mouse, anti-rabbit, and anti-rat antibodies from Invitrogen (Thermo Fisher Scientific, Waltham, MA) were used. Unless otherwise specified, general reagents and chemicals were from Sigma-Aldrich, and reagents for cell cultures were from Invitrogen.

ATR, p-CHK1 (S345), RPA70, and p-histone H3 (Ser10) antibodies were obtained from Cell Signaling; ATRIP, NUP153, and RPA32 were obtained from Abcam; TPR, γH2AX (S139), and lamin A Abs were obtained from Sigma and Millipore; and Chk1 was obtained from Leica. EdU (click-IT), RPA70-, TopBP1-, and RAD17-small hairpin RNAs (shRNAs) were obtained from Invitrogen and Origene Technologies, respectively. ATR-specific inhibitor and ATR shRNA were from Dr. Oscar Capetillo (Centro Nacional de Oncologia [CNIO]) (Toledo et al., 2011 (link)); the GFP-ATR plasmid was from Dr. Randal Tibbetts (Tibbetts et al., 2000 (link)); RFP-Lamin was from Prof. Howard J. Worman (Columbia University) (Ostlund et al., 2006 (link)); and RFP-Nucleophosmin was a gift by Dr. Michelle Hill (University of Queensland). GFP-H2B and m-cherry-H2B were from IFOM. Colchicine, α-tubulin Ab, Leptomycin B, Hydroxy Urea, sorbitol, Aphidicholine, and R3306 CDK1-specific inhibitor were obtained from Sigma.