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2 protocols using plastic backedsilica gel tlc plates z122777 25ea

1

Synthesis and Characterization of Pyridyl Porphyrins

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meso-Tetrakis(2-N-pyridyl)porphyrin (H2T-2-PyP) and meso-tetrakis(3-N-pyridyl)porphyrin (H2T-3-PyP) were purchased from Frontier
Scientific. Ethyl p-toluenesulfonate (98%) was from
Sigma-Aldrich. The n-octyl p-toluenesulfonate
and methyl-tri-n-octylammonium chloride (>95%) were from TCI America.
MnCl2·4H2O (99.7%) was supplied by J. T.
Baker, FeCl2 (98%) was from Sigma-Aldrich, and NH4PF6 (99.99%) was from GFS chemicals. Anhydrous diethyl
ether and acetone were from EMD chemicals, while dichloromethane,
chloroform, acetonitrile, EDTA, and KNO3 were purchased
from Mallinckrodt. Anhydrous N,N-dimethylformamide
(DMF) of 99.8% purity (kept over 4-Å molecular sieves) and plastic-backed
silica gel TLC plates (Z122777-25EA) were from Sigma-Aldrich. Xanthine,
equine ferricytochrome c (lot 7752), and (+)-sodium l-ascorbate (>98%) were from Sigma, whereas xanthine oxidase
was prepared by R. Wiley.1 (link) Triethylamine
(Et3N) of >99.5% purity was obtained from Thermo Scientific
Pierce. All chemicals were used as received without further purification.
The 1H NMR spectra were recorded on a spectrometer “Mercury
Varian 300” with deuterated chloroform as solvent.
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2

Synthesis and Purification of Manganese Porphyrins

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Porphyrin ligands, H2T-2-PyP, H2T-3-PyP and H2T-4-PyP, were purchased from Frontier Scientific. Reagents needed for synthesis and purification of MnPs were purchased from the sources detailed in [42 (link), 50 (link)]. Plastic-backed silica gel TLC plates (Z122777-25EA) and (+)-sodium L-ascorbate (>98 %) were from Sigma-Aldrich. Acetonitrile (CH3CN) and KNO3 were purchased from Mallinckrodt. All chemicals were used as received without prior purification. All chemicals used to measure cellular thiol/disulfide redox states including reduced and oxidized glutathione, cysteine, cysteine, dansylchloride, iodoacetic acid, acetic acid, perchloric acid and methanol were purchased from Sigma-Aldrich.
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