The following antibodies were purchased from BD Pharmingen, unless otherwise noted: BV421-conjugated anti-CD3 (#562426); BV421-conjugated anti-CD4 (BioLegend, #300532); BV421-conjugated anti-CD56 (BD Horizon, #562751); BV421-conjugated anti-CD86 (#562432); BV421-conjugated anti-HLA-DR (BD Horizon, #562804); BV421-conjugated IgG1, κ Isotype Control (BioLegend, #400158); BV421-conjugated IgG2a, κ Isotype Control (BD Horizon, #562439); BV421-conjugated IgG2b, κ Isotype Control (BioLegend, #400342); FITC-conjugated anti-CD4 (#555346); FITC-conjugated anti-CD14 (#555397); FITC-conjugated anti-CD15 (#562370); FITC-conjugated anti-CD19 (#555412); FITC-conjugated anti-CD25 (#555431); FITC-conjugated anti-CD69 (#555530); FITC-conjugated IgG1, κ Isotype Control (#555748); FITC-conjugated IgG2a, κ Isotype Control (#555573); PE-conjugated anti-CD8 (#555367); PE-conjugated anti-CD11b (BioLegend, #301406); PE-conjugated anti-Cd11c (#555392); PE-conjugated IgG1, κ Isotype Control (#555749); APC-conjugated anti-CD3 (#555335); APC-conjugated anti-CD11a (R&D Systems, #FAB3595A); APC-conjugated anti-HLA-DR (#559866); APC-conjugated IgG1, κ Isotype Control (#555751); and APC-conjugated IgG2a, κ Isotype Control (BioLegend, #400220).
Fitc conjugated anti cd25
Manufactured by BioLegend
Sourced in United States
FITC-conjugated anti-CD25 is a monoclonal antibody that binds to the CD25 (IL-2Rα) antigen on the surface of cells. The antibody is conjugated to the fluorescent dye FITC, which allows for the detection and analysis of CD25-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Bv421 conjugated anti cd4, by BioLegend (1 mentions)
Fitc conjugated anti cd14, by BioLegend (1 mentions)
Fitc conjugated anti cd19, by BioLegend (1 mentions)
Fitc conjugated anti cd69, by BioLegend (1 mentions)
Pe conjugated anti cd8, by BioLegend (1 mentions)
Pe conjugated anti cd11b, by BioLegend (1 mentions)
Apc conjugated anti hla dr, by BioLegend (1 mentions)
Fitc conjugated anti cd4, by BioLegend (1 mentions)
Bv421 conjugated anti cd3, by BioLegend (1 mentions)
Anti human foxp3 staining set, by Thermo Fisher Scientific (1 mentions)
Epics xl, by Beckman Coulter (1 mentions)
Fluorescein isothiocyanate fitc conjugated anti cd4, by BioLegend (1 mentions)
Pe conjugated anti cd83, by BioLegend (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Percp cy5.5 conjugated anti cd4, by BioLegend (1 mentions)
Fitc conjugated anti ifn γ, by BioLegend (1 mentions)
Percp cy5.5 conjugated anti cd8, by BioLegend (1 mentions)
Apc conjugated anti il 17, by BioLegend (1 mentions)
3 protocols using fitc conjugated anti cd25
1
Multiparameter Flow Cytometry Panel
2
Phenotypic Characterization of Activated T Cells
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Non-adherent lymphocytes or purified CD4+ T cells were collected at 0, 1, 2 and 3 days following stimulation, and the cells were stained with phycoerythrin (PE)-conjugated anti-CD83, fluorescein isothiocyanate (FITC)-conjugated anti-CD4 or FITC-conjugated anti-CD25 (All from BioLegend, San Diego, CA, USA) and incubated on ice for 30 min in the dark. The controls were prepared using FITC- or PE-conjugated isotype controls. The intracellular staining of TGF-β-treated CD4+ T cells with anti-Foxp3 mAb was performed using an anti-human Foxp3 staining set (eBioscience, San Diego, CA, USA) according to the manufacturer's instructions. The cells were subsequently analyzed using a flow cytometer (Beckman Coulter Epics XL; Beckman Coulter, Miami, FL, USA) by using a two-color acquisition method and the data were analyzed by using FlowJo 7.6.1 software (Treestar, Inc., Ashland, OR, USA).
3
Profiling T Cell Phenotypes and Cytokines
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We isolated mononuclear cells from fresh LN samples, as previously described [28 (link)]. Phenotypic & cytokine analysis of T cells was performed using FACSCalibur flow cytometer (BD biosciences, USA). The following mouse anti-human monoclonal antibodies (mAbs) were used: PerCp-Cy5.5 conjugated anti-CD4 (Clone: RPA-T4), PerCp-Cy5.5 conjugated anti-CD8 (Clone: SK1), Fluorescein isothiocyanate (FITC)-conjugated anti-CD25 (Clone: M-A251), Allophycocyanin (APC)-conjugated anti-CD127 (Clone: A019D5), FITC-conjugated anti-IFN-γ (Clone: 4S.B3), PE anti-human TGF-β (Clone: TW4-9E7), APC-conjugated anti-IL-17 (Clone: BL168), APC-conjugated anti-IL-4 (Clone: 8D4-8), APC-conjugated anti-IL-10 (Clone: JES3-19F1), and their respective isotype controls were purchased from Biolegend, USA. Phycoerythrin (PE)-conjugated anti-Foxp3 (Clone: 259 D/C7), PE-conjugated anti-tumor necrosis factor-α (TNF-α) (Clone: MAB11) and their isotype controls were obtained from BD biosciences.
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