Dxc800
Manufactured by Beckman Coulter
Sourced in United States
The DXC800 is a fully automated clinical chemistry analyzer designed for high-throughput testing in clinical laboratories. It offers fast, precise, and reliable analysis of a wide range of clinical chemistry parameters.
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68 protocols using dxc800
1
Serum Cholesterol and Triglycerides Analysis
2
Evaluating Kidney Injury Biomarkers in SYNERGY
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SYNERGY will include kidney damage as a secondary outcome using 24 hr urine samples (gold standard) for measuring proteinuria and albuminuria [45 (link)]. Participants will be provided with bottles for collection and educated on correct collection procedures in accordance with standard protocol. Urinary total protein (pyrogallol red) and urinary albumin (turbidimetric method based on antibody-antigen complexes) will be measured in timed-samples (mg/24 hrs) and cross-checked against creatinine ratios (protein and albumin, respectively) on Beckman DxC800 general chemistry analyser (Beckman Coulter, Brea, CA, USA) [46 ].
Kidney function using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula will also be measured [22 ].
In addition, urinary Kim-1, a marker of kidney tubule damage, will be measured from midstream urine collections before and after each intervention [47 (link)]. Samples will be stored at −80°C, followed by analysis using a commercially available sandwich ELISA according to the manufacturer’s instructions (USCN Life Sciences, Wuhan, PRC). Significant changes in Kim-1 have been achieved in a 6 week dietary intervention study indicating the feasibility of Kim-1 as a sensitive marker in SYNERGY [48 (link)].
Kidney function using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula will also be measured [22 ].
In addition, urinary Kim-1, a marker of kidney tubule damage, will be measured from midstream urine collections before and after each intervention [47 (link)]. Samples will be stored at −80°C, followed by analysis using a commercially available sandwich ELISA according to the manufacturer’s instructions (USCN Life Sciences, Wuhan, PRC). Significant changes in Kim-1 have been achieved in a 6 week dietary intervention study indicating the feasibility of Kim-1 as a sensitive marker in SYNERGY [48 (link)].
3
Birth Cohort and ALT Levels for Anti-HCV Testing
4
Serum Extraction and Biochemical Analysis
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Blood samples were transferred to 1.5 mL tubes and allowed to clot. Then, serum was separated by centrifugation at 4000 rpm for 15 min. SCr and BUN were measured using a Beckman Coulter Dxc800 biochemical analyzer (Beckman Coulter).
5
Maternal and Fetal Biochemical Profiling
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Classical biochemistry (triglycerides, cholesterol, glycaemia, urea, creatinine, ASAT, ALAT) was performed on maternal (n = 3 C and 9 E) and fetal EDTA-plasma (n = 12 CC and 12 EC) using a classical automated biochemistry analyser (DXc800 Beckman Coulter equipment). Plasma insulin was assayed with chemiluminescent immunoassay using paramagnetic particles on an automaton analyser (DXi Beckman Coulter equipment).
6
Serum Biomarkers in Inflammatory Bowel Disease
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A total of 5 ml serum was collected without an anticoagulant from the patients with IBD and the health donors. The blood samples were centrifuged at 2750 rpm for 12 minutes at room temperature (RT). The serum was collected and stored at −80°C until use. All the hematological indexes were measured by an automatic biochemical analyzer. The levels of TNFα, IL-6, and IL-10 in the plasma were detected using a chemiluminescence immune detection system (Immulite1000, Siemens, Germany). The estradiol and testosterone levels were detected using an automatic chemiluminescence immunoanalyzer (DX1801, Beckman, USA). White blood cells (WBC), red blood cells (RBC), platelets (PLT), hemoglobin (Hgb), C reactive protein (CRP), and the erythrocyte sedimentation rate (ESR) were detected by an automatic biochemical analyzer (XE2100, Sysmex, Japan). Albumin levels were recorded by an automatic biochemical analyzer (DXC800, Beckman, USA).
7
Comprehensive Clinical Data Collection for PCI Patients
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Basic data were collected, including demographics, medical history, use of medications, number of diseased vessels, the contrast volume used and left ventricular ejection fraction (LVEF) as measured by echocardiography, as well as laboratory findings including Scr levels prior to, as well as 24 and 48 h after PCI, as measured using an automated biochemistry analyzer (DXC800; Beckmann Coulter, Inc.) at the hospital's clinical laboratory. The eGFR was calculated using the Chronic Kidney Disease (CKD) Epidemiology Collaboration formula (16 (link)).
8
Gestational Diabetes Mellitus Screening
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Pre-pregnancy BMI (kg/m2) was calculated on the basis of self-reported weight measurements before the pregnancy.
Venous blood samples were drawn after a minimum of a 10-h overnight fast during 24-28th gestational weeks. Serum biochemical parameters were measured using a Beckman DXC800 automatic biochemical analyser. Haemoglobin A1C (HbA1c) levels were measured using high-performance liquid chromatography (TOSOH HLC-723 G7, Japan), and insulin levels were determined using an insulin detection kit and its reagent (Roche Diagnostics, Germany).
One-step 75 g OGTTs were performed during 24-28th gestational weeks. Fasting and postload blood glucose and insulin levels were measured at 0, 60, and 120 min. GDM was diagnosed according to the 2013 World Health Organization diagnostic criteria: fasting blood glucose ≥5.1 mmol/L,1-h OGTT blood glucose ≥10.0 mmol/L, or 2-h OGTT blood glucose ≥8.5 mmol/L.
All participants were inpatients before delivery. Weight gain during pregnancy was collected, and primary adverse outcomes were confirmed by experienced obstetricians. The natural rupture of foetal membranes before the first stage of labour was diagnosed as PROM. All subjects had delivered and finished follow-up by December 2019.
Venous blood samples were drawn after a minimum of a 10-h overnight fast during 24-28th gestational weeks. Serum biochemical parameters were measured using a Beckman DXC800 automatic biochemical analyser. Haemoglobin A1C (HbA1c) levels were measured using high-performance liquid chromatography (TOSOH HLC-723 G7, Japan), and insulin levels were determined using an insulin detection kit and its reagent (Roche Diagnostics, Germany).
One-step 75 g OGTTs were performed during 24-28th gestational weeks. Fasting and postload blood glucose and insulin levels were measured at 0, 60, and 120 min. GDM was diagnosed according to the 2013 World Health Organization diagnostic criteria: fasting blood glucose ≥5.1 mmol/L,1-h OGTT blood glucose ≥10.0 mmol/L, or 2-h OGTT blood glucose ≥8.5 mmol/L.
All participants were inpatients before delivery. Weight gain during pregnancy was collected, and primary adverse outcomes were confirmed by experienced obstetricians. The natural rupture of foetal membranes before the first stage of labour was diagnosed as PROM. All subjects had delivered and finished follow-up by December 2019.
9
Fasting Biomarker Quantification in Serum
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Venous blood was collected in the morning after approximately 12 h overnight fasting and left to clot for 30 min at room temperature. The serum samples were obtained by centrifugation at 3000 rpm for 10 min at 4 °C and stored at −20 °C until analysis.
Serum ghrelin and PYY levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits (Jianglaibio, Shanghai, China). Serum triglycerides, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and insulin levels were measured with a fully automatic immunoanalyzer (Beckman DXC 800, Fullerton, CA, USA). Fasting plasma glucose level (FPG) was measured by dry chemical method.
Serum ghrelin and PYY levels were quantified using enzyme-linked immunosorbent assay (ELISA) kits (Jianglaibio, Shanghai, China). Serum triglycerides, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, and insulin levels were measured with a fully automatic immunoanalyzer (Beckman DXC 800, Fullerton, CA, USA). Fasting plasma glucose level (FPG) was measured by dry chemical method.
10
Blood Biomarker Quantification Protocol
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Forty-eight hours after the final administration, the eyeballs were removed to collect 0.8–1 ml blood, indoor solidification for 30 min, and the supernatant was collected after centrifugation (5,000 × g at 20°C for 5 min). The ELISA kit was used to determine IL-1, IL-6, TNF-α and other indexes, and a fully automatic biochemical analyzer DXC800 (Beckman Coulter, Inc., Brea, CA, USA) was used to determine all the indexes (14 –17 (link)) of blood biochemistry.
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