Benzo a pyrene bap

Benzo[b]fluoranthene (BbF, CAS 205-99-2), benzo[k]fluoranthene (BkF, CAS 207-08-9), benzo[a]pyrene (BaP, CAS 50-32-8), indeno[1,2,3-cd]pyrene (IcdP, CAS 193-39-5), nitric acid (HNO₃, CAS 7697-37-2), hydrochloric acid (HCl, CAS 7647-01-0), hydrofluoric acid (HF, CAS 7664-39-3), and perchloric acid (HClO₄, CAS 7601-90-3) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Fluoranthene (FR, CAS 206-44-0) and pyrene (PY, CAS 129-00-0) were obtained from Nacalai Tesque Inc. (Kyoto, Japan). Benz[a]anthracene (BaA, CAS 56-55-3), dibenz[a,h]anthracene (DahA, CAS 53-70-3), 1-nitropyrene (CAS 5522-43-0), and 2-acetylaminofluorene (CAS 53-96-3) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Chrysene (CHR, CAS 218-01-9), benzo[ghi]perylene (BghiP, CAS 191-24-2), phenobarbital (CAS 50-06-6), and β-naphthoflavone (CAS 6051-87-2) were purchased from Sigma-Aldrich Co. LLC (St. Louis, MO, USA). Quartz filters were obtained from Pall Life Sciences (Port Washington, NY, USA).

Pefcalcitol (development code: SMD-502, 2-{[(1S,3R,5Z,7E,20S)-1,3-dihydroxy-9,10-secopregna-5,7,10(19),16-tetraen-20-yl]oxy}-N-(2,2,3,3,3-pentafluoropropyl)acetamide, CAS No. 381212-03-9) was synthesized by Chugai Pharmaceutical Co., Ltd. The purity was >97% when measured with HPLC. Mitomycin C (MMC, CAS No. 50-07-7, Kyowa Hakko Kirin Co., Ltd., Tokyo, Japan) was used as a reference article for a CA test and an MN test. MMC was dissolved in distilled water and diluted with saline for the CA and bone marrow MN tests, or dissolved in a mixture of acetone and olive oil (4:1 by volume) for the skin MN test. Benzo[a]pyrene (B[a]P, CAS No. 50-32-8, Wako Pure Chemical Industries, Ltd., Osaka, Japan), which was used as a reference article in the CA test, was dissolved in dimethyl sulfoxide (DMSO).