The chromogenic substrate S-2238 was purchased from Chromogenix (Milano, Italy). Normal control human plasma “Plasma Control N” was obtained from Siemens Healthcare (Malvern, PA). Phosphatidyl-L-serine was purchased from MP Biomedicals. 4 nm silica nanoparticles dispersion was obtained from Alfa Aesar (Ward Hill, MA). Silica nanoparticle dispersion of 7 nm (Ludox SM), 12 nm (Ludox HS), and 22 nm (Ludox TM) were purchased from Sigma-Aldrich. 50 nm (NexSil 85-40) and 85 nm (NexSil 125-40) silica nanoparticle dispersions were purchased from Nyacol Nano Technologies, Inc. (Ashland, MA). MPC polymer (poly(2-methacryloyloxyethyl phosphorylcholine) (Lipidure-CM5206) and MPC polymer coated 96 well plate “Lipidure-Coat A-F96” was purchased from NOF Corporation (Tokyo, Japan). FXII was purchased from Haematologic Technologies Inc. (Essex Junction, VA) and used as received.
Ludox hs
Manufactured by Merck Group
Sourced in United Kingdom
Ludox HS is a colloidal silica product manufactured by Merck Group. It is a stable, aqueous dispersion of spherical silica particles. The core function of Ludox HS is to serve as a precursor for the synthesis of various silica-based materials and coatings.
Automatically generated - may contain errors
Lab products found in correlation
Lipidure cm5206, by NOF corporation (3 mentions)
Ludox sm, by Merck Group (1 mentions)
Ludox tm, by Merck Group (1 mentions)
S 2238, by Werfen (1 mentions)
Hydrogen peroxide solution, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Toluene, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
3 protocols using ludox hs
1
Silica Nanoparticle-Mediated FXII Activation Assay
2
Synthesis of Silica Nanoparticles
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Acetone, ammonia, hexamethyldisilzane (HMDS), HCl, 30% hydrogen peroxide solution, KOH, methanol, NaCl, and toluene were all purchased from Sigma-Aldrich Chemical Co. Silica nanoparticles prepared using LUDOX-HS (40% wt SiO2) were generously gifted by the Thermo Fisher research and development team.
3
Stabilizing Oil-in-Water Emulsions
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All oil-in-water (O/W) emulsions were prepared by using de-ionised water obtained from a reverse osmosis filtration system as the continuous phase. Commercial sunflower oil (viscosity = 50 cP) was purchased by a local retailer and used as the dispersed phase. Both Polysorbate20, i.e. Tween20, (Hydrophilic Lipophilic Balance, HLB =16.70 and molecular weight=1227.54 g/mol, critical micelle concentration = 0.06 mM) and a 30 wt.% suspension of silica in Water (Ludox HS, surface area 220 m 2 /g and density 1.21 g/mL) were supplied by Sigma-Aldrich Company (UK) and used as emulsifiers.
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